CN-122005417-A - Method for skin photoaging by combining exosomes with yellow light care and application

Abstract

The invention relates to the technical field of skin care, and provides a method for nursing photoaged skin by combining exosomes with yellow light, which comprises the following steps of S1, preparing exosomes, separating exosomes from a mesenchymal stem cell supernatant; S2, skin pretreatment, namely creating a skin micro-channel by using a medical microneedle after aseptic cleaning and local anesthesia are carried out on an photoaged skin area, S3, delivering an exosome, namely smearing the exosome prepared in the step S1 on the pretreated photoaged skin area, standing until the skin is completely absorbed, and S4, irradiating the photoaged skin area by using yellow light with the wavelength of 590-640nm for 5 minutes. The method and the application of the exosome combined with yellow light to care photo-aged skin can remarkably improve photo-aged skin and avoid adverse reactions in the nursing process.

Inventors

• ZHU YIZHUN
• LI ZHAOYI
• CHEN XIN
• SUN WEN
• LUO YUN

Assignees

• 成都拜美森生物科技有限公司
• 澳门科技大学

Dates

Publication Date

20260512

Application Date

20260228

Claims (6)

1. 1. A method for nursing photoaged skin by combining exosomes with yellow light is characterized by comprising the following steps: s1, preparing exosomes, namely separating the exosomes from the supernatant of the mesenchymal stem cells; S2, skin pretreatment, namely creating a skin micro-channel by adopting a medical micro-needle after aseptic cleaning and local anesthesia are carried out on an photoaged skin area; s3, exosome delivery, namely smearing the exosome prepared in the step S1 on the pretreated photoaged skin area, and standing until the exosome is completely absorbed; and S4, yellow light irradiation, namely irradiating the photoaged skin area by adopting yellow light with the wavelength of 590-640nm, wherein the irradiation time is 5 minutes.
2. 2. The method for the combined yellow light care of photoaged skin according to claim 1, wherein in step S1, the mesenchymal stem cells are umbilical cord mesenchymal stem cells.
3. 3. A method for the combined yellow light care of photoaged skin of an exosome according to claim 1, wherein in step S1, the identity of the exosome is confirmed by morphological evaluation by transmission electron microscopy, particle size distribution analysis by nanoparticle tracking analysis, and detection of a characteristic marker by Western blotting.
4. 4. A method of treating photoaged skin with exosome combination according to claim 3, wherein the characteristic markers comprise CD63, CD81, and TSG101.
5. 5. Use of the method according to any one of claims 1-4 for the preparation of a photoaged skin care product.
6. 6. The method of claim 1 to 4, wherein the device integrates an exosome delivery module with a yellow light irradiation module, and the wavelength of the yellow light irradiation module can be precisely regulated and controlled between 590 nm and 640nm.

Description

Method for skin photoaging by combining exosomes with yellow light care and application Technical Field The invention relates to the technical field of skin care, in particular to a method for nursing photoaged skin by combining exosomes with yellow light and application of the method. Background Ultraviolet (UV) radiation is a major environmental driver of skin photoaging, and is manifested clinically as wrinkles, sagging, dryness and increased skin fragility. UVA (315-400 nm) and UVB (280-315 nm) are the most bioactive wavelengths in the ultraviolet spectrum. After uv penetration through the skin, direct deoxyribonucleic acid (DNA) damage and indirect oxidative stress are initiated, mainly by the overproduction of Reactive Oxygen Species (ROS) in keratinocytes and dermal fibroblasts, thus initiating a complex molecular cascade. ROS proliferation initiates inflammatory cascades including pro-inflammatory cytokine upregulation, cyclooxygenase 2 (COX 2) induction, and inflammatory cell infiltration, as well as immunosuppression and extracellular matrix (ECM) degradation. In particular, inflammation is generally caused by a combination of up-regulation of pro-inflammatory cytokine expression, induction of COX2 and infiltration of inflammatory cells. At the same time, reactive Oxygen Species (ROS) stimulate the expression of Matrix Metalloproteinases (MMPs), degrading collagen and other extracellular matrix (ECM) proteins, while inhibiting transforming growth factor-beta (TGF-beta) signaling that maintains the integrity of dermal connective tissue. This imbalance can lead to vicious circle of inflammation and tissue degradation, ultimately accelerating the photoaging process. Current photo-aging skin care means including topical retinoic acid, chemical skin change, laser care, etc., can generally improve significantly in a short period of time, but also have limitations such as irritation, redness, and dryness. It is difficult to maintain the care effect for a long period of time due to the persistence of photoaging and the potential reverse effect of ultraviolet re-irradiation. The reactions during care are varied and common adverse reactions include mild irritation, blisters and changes in pigmentation. Long-term care often requires cumbersome care steps and continuous follow-up, which places a heavy burden on the patient. Therefore, finding a care method that can significantly improve photoaged skin while avoiding adverse reactions occurring during the care process is a current urgent problem to be solved. Disclosure of Invention Aiming at the defects in the prior art, the invention aims to provide a method for nursing photoaged skin by combining exosomes with yellow light and application thereof, which can obviously improve the photoaged skin and avoid adverse reactions in the nursing process. The invention provides a method for nursing photoaged skin by combining exosomes with yellow light, which comprises the following steps: s1, preparing exosomes, namely separating the exosomes from the supernatant of the mesenchymal stem cells; S2, skin pretreatment, namely creating a skin micro-channel by adopting a medical micro-needle after aseptic cleaning and local anesthesia are carried out on an photoaged skin area; s3, exosome delivery, namely smearing the exosome prepared in the step S1 on the pretreated photoaged skin area, and standing until the exosome is completely absorbed; and S4, yellow light irradiation, namely irradiating the photoaged skin area by adopting yellow light with the wavelength of 590-640nm, wherein the irradiation time is 5 minutes. Further, in the step S1, the exosome separation step is that the supernatant of the mesenchymal stem cells is centrifuged by a centrifugal force of 300 Xg for 10 minutes, a centrifugal force of 2000 Xg for 20 minutes, a centrifugal force of 10000 Xg for 30 minutes to remove cell fragments, and then centrifuged by a centrifugal force of 100000 Xg for 90 minutes to obtain an exosome precipitate, and the exosome precipitate is filtered by a membrane of 0.22 μm and stored at-80 ℃. Further, in step S1, the mesenchymal stem cells are umbilical cord mesenchymal stem cells. Further, in step S1, morphological evaluation is performed by transmission electron microscopy, particle size distribution analysis is performed by nanoparticle tracking analysis, and characteristic markers are detected by western blotting to confirm the identity of the exosomes. Further, the characteristic markers include CD63, CD81, and TSG101. The invention provides application of a method for nursing photoaged skin by combining exosomes with yellow light in preparing a photoaged skin care product. The invention provides application of a method for nursing photo-aged skin by combining exosomes with yellow light, which is used for preparing skin photo-aging nursing equipment, wherein the equipment integrates an exosome delivery module and a yellow light irradiation module, and the wavelength of