CN-122005418-A - Gel loaded with exosome composition and application thereof

Abstract

The invention belongs to the technical field of exosome application, and discloses gel loaded with an exosome composition and application thereof. The invention discovers that the exosome composition has obvious promotion effect on proliferation and migration of human immortalized keratinocytes through specific combination of three exosomes, and then discovers that the exosome composition has obvious promotion effect on FLG expression of cells and obvious reduction effect on pro-inflammatory factors of the cells through later detection, so that the exosome composition can be used for repairing damaged barriers of the cells. In the application aspect, the exosome composition and the freeze-drying protective agent are mixed to prepare the exosome composition into freeze-dried powder, the freeze-dried powder is prepared into a gel preparation convenient for skin use in use, and the application effect of the gel preparation is verified through a constructed mouse model, so that the gel preparation containing the exosome composition prepared by the method has the effect of obviously repairing damaged skin barrier after photodamage, and is expected to be widely applied.

Inventors

• WEI XIN
• DENG GUANGWEN
• WU SHUXIA
• LI YUXIA
• SUN QI

Assignees

• 艾一生命科技(广东)有限公司

Dates

Publication Date

20260512

Application Date

20260311

Claims (9)

1. 1. The exosome composition is characterized by comprising umbilical mesenchymal stem cell exosomes, honeysuckle exosomes and green tea exosomes in a weight ratio of 2-3:0.5-0.8:1-2.
2. 2. Use of the exosome composition of claim 1 for the development of a product for alleviating and/or treating an impaired skin barrier.
3. 3. The use according to claim 2, wherein the exosome composition has at least one of the following effects: (1) Promote proliferation and/or migration of human immortalized keratinocytes; (2) Promoting human immortalized keratinocyte FLG expression; (3) Inhibiting expression of human immortalized keratinocyte inflammatory factor.
4. 4. The lyoprotectant of the exosome composition of claim 1, wherein the lyoprotectant consists of 0.3-0.5% v/v ceramide, lysine and 1.8-1.5% v/v glycerol, wherein the final concentration of lysine is 70-80mM after the exosome lyoprotectant and exosome are mixed.
5. 5. Use of an exosome composition according to claim 1 and a lyoprotectant according to claim 4 for the preparation of a product for alleviating and/or treating an impaired skin barrier.
6. 6. A gel formulation comprising the exosome composition of claim 1 and the lyoprotectant of claim 4.
7. 7. Use of a gel formulation according to claim 6 for the preparation of a product for alleviating and/or treating an impaired skin barrier.
8. 8. The use according to claim 7, wherein the damaged skin barrier is damaged by uv irradiation.
9. 9. The use according to claim 8, wherein the damage to the skin barrier caused by uv irradiation is manifested by an increase in the TWEL value and a decrease in the water content of the stratum corneum.

Description

Gel loaded with exosome composition and application thereof Technical Field The invention belongs to the technical field of exosome application, and in particular relates to gel loaded with exosome composition and application thereof. Background The skin barrier, particularly the stratum corneum, which is the outermost layer of the epidermis, is the first line of physical, chemical and biological defense between the human body and the external environment. Its structural integrity is critical to maintaining skin homeostasis, preventing excessive loss of moisture, and protecting against invasion of external stimuli, allergens and microorganisms. In recent years, with increased environmental pressure, popularization of inappropriate skin care behaviors, and increased incidence of related skin diseases, damage to the skin barrier has become a research hotspot and a common clinical problem in the field of skin science. Exosomes (Exosomes) have recently become the leading edge of research in the fields of skin science and regenerative medicine as an extracellular vesicle secreted by cells and having a diameter of about 30-150 nm. The cell-based recombinant DNA can carry bioactive substances such as protein, lipid, mRNA, miRNA, circRNA and the like of source cells, and can be taken up by receptor cells, so that the biological functions of target cells can be accurately regulated and controlled, and the cell-based recombinant DNA has unique application potential in the aspects of skin repair, regeneration and rejuvenation. The function of exosomes has a "homing effect" and a "content-dependent" and its biological effect depends to a large extent on the type and state of its parent cells. Currently, exosomes for skin improvement studies are mainly derived from the following cell types, 1, mesenchymal stem cells, which are the most widely and most deeply studied and used sources at present. Typically from fat, bone marrow and umbilical cord, 2, skin-derived adult cells, which are more "tissue specific" and which signal more readily recognized and responded to by skin cells, 3, plant-derived extracellular vesicles, such as grape, ginger, broccoli, ginseng, apple and the like, which have good biocompatibility and low immunogenicity. Is rich in plant-specific antioxidants (such as polyphenols, flavonoids) and bioactive molecules. Mainly shows excellent oxidation resistance and anti-inflammatory capability, can be used for resisting ultraviolet-induced oxidation injury and skin inflammation, and is concerned in 'pure beauty' and green cosmetic formulations. However, the above animal-derived cells are difficult to separate and purify, have high cost and small cell number, and some of them involve ethics, and are currently used in the laboratory research stage or in small scale for skin improvement, thereby limiting their wide application. The exosome gel is used as an innovative delivery system and application dosage form, combines the biological activity of the exosome and the physical property of the gel material, and is the hot spot direction of the current transformation application. Liquid exosome formulations are prone to run off the skin surface and have unstable permeation efficiency. The gel matrix can be anchored on an application part to form a drug reservoir, and the exosome is slowly and continuously released, so that the acting time of the exosome is greatly prolonged, and the bioavailability is improved. This is critical for wound repair and anti-aging applications requiring sustained action. However, the preparation process of the exosome gel preparation is complex, the technical requirement is high, the exosome is uniformly and stably loaded into the gel matrix without damaging the integrity and the activity of the exosome gel preparation, and the technical requirement is extremely high. Parameters such as mixing temperature, pH, shear force, etc. may affect exosome activity. Disclosure of Invention Based on the above-mentioned drawbacks of the prior art, the present invention first provides an exosome composition for skin barrier damage. The aim of the invention is achieved by the following technical scheme: The exosome composition consists of umbilical mesenchymal stem cell exosome, honeysuckle exosome and green tea exosome in the weight ratio of 2-3 to 0.5-0.8 to 1-2. The umbilical cord mesenchymal stem cell exosome, the honeysuckle exosome and the green tea exosome are prepared by adopting a conventional method in the field. The exosome composition is used for immortalizing keratinocytes of human, and has obvious promotion effect on proliferation and migration of the cells, and the FLG expression level of the detected cells is obviously increased; the epidermal cell layer is the most important cell layer of the skin barrier, and the proliferation activity and migration activity of the epidermal cells are directly related to the repair ability after the skin barrier is damaged. The exosome composition