CN-122005432-A - Preparation method of natural antibacterial neutral agent based on PLGA slow release mechanism

CN122005432ACN 122005432 ACN122005432 ACN 122005432ACN-122005432-A

Abstract

The invention relates to the technical field of natural antibacterial neutral agent preparation, and discloses a preparation method of a natural antibacterial neutral agent based on a PLGA slow-release mechanism, which comprises the steps of taking polylactic acid PLGA as a carrier, mixing meloxicam polylactic acid microspheres, a chitosan solution and a cross-linking agent solution, adding a surfactant, and stirring until uniform emulsion is formed; adding the emulsion into a hardener, stirring until the crosslinking reaction is completed to obtain chitosan microspheres, washing the chitosan microspheres with deionized water to remove surface residual surfactant and crosslinking agent, and drying the chitosan microspheres in a ventilated drying chamber to obtain the natural antibacterial neutral agent based on PLGA slow-release mechanism. The slow release effect of the medicine can be realized by using PLGA as a carrier to prepare the meloxicam polylactic acid microsphere. This helps the sustained release of the drug at the skin surface, thereby maintaining the balance of skin pH for a longer period of time, promoting repair of skin barrier and maintenance of microbial balance.

Inventors

Jin Yazhe
ZHANG LINNAN
GUO LANG

Assignees

沈阳医学院

Dates

Publication Date: 20260512
Application Date: 20251231

Claims (6)

1. A preparation method of a natural antibacterial neutral agent based on PLGA slow release mechanism is characterized by comprising the following specific steps: Firstly, taking polylactic acid PLGA as a carrier, weighing polylactic acid PLGA, dissolving in an organic solvent, then weighing meloxicam, adding into the solution, stirring and dissolving to form an oil phase, dripping the oil phase into a dispersing agent at a constant speed by a medical injector in a magnetic stirring state, volatilizing the organic solvent at normal temperature to solidify the microspheres, washing the obtained microsphere suspension, centrifuging, and freeze-drying the centrifuged microspheres to obtain meloxicam polylactic acid microspheres; Step two, adding chitosan powder into acetic acid solution, and stirring until the chitosan powder is completely dissolved to obtain chitosan solution; step three, adding the cross-linking agent into water, and stirring until the cross-linking agent is completely dissolved to obtain a cross-linking agent solution; Mixing meloxicam polylactic acid microspheres, chitosan solution and cross-linking agent solution, adding a surfactant, stirring to form uniform emulsion, adding the emulsion into a hardening agent, stirring until the cross-linking reaction is completed to obtain chitosan microspheres, washing the chitosan microspheres with deionized water to remove the surfactant and the cross-linking agent remained on the surface, and drying the chitosan microspheres in a ventilated drying chamber to obtain the natural antibacterial neutral agent based on PLGA slow-release mechanism.
2. The method for preparing the natural antibacterial neutral agent based on the PLGA slow release mechanism according to claim 1, wherein in the first step, the organic solvent is a mixture of dichloromethane and acetone.
3. The method for preparing the natural antibacterial neutral agent based on the PLGA slow release mechanism according to claim 2, wherein in the first step, the dispersing agent is a polyvinyl alcohol solution.
4. The method for preparing the natural antibacterial neutral agent based on the PLGA slow release mechanism according to claim 3, wherein in the first step, the meloxicam polylactic acid microspheres are stored at 4 ℃.
5. The method for preparing a natural antibacterial neutral agent based on PLGA slow release mechanism according to claim 4, wherein in the fourth step, the cross-linking agent is ammonium sulfate.
6. The method for preparing a natural antibacterial neutral agent based on PLGA slow release mechanism according to claim 5, wherein in the fourth step, the surfactant is Tween-80.

Description

Preparation method of natural antibacterial neutral agent based on PLGA slow release mechanism Technical Field The invention relates to the technical field of natural antibacterial neutral agent preparation, in particular to a preparation method of a natural antibacterial neutral agent based on a PLGA slow release mechanism. Background The normal pH value of the skin surface is the representation of the good physiological state of the skin, and researches show that the pH value of the skin surface is 5.5, which is the optimal pH value of the skin. When the skin epidermis barrier is damaged, the pH value of the skin surface is higher, and when the pH value of the skin is regulated to be in an optimal range, the repair of the skin barrier can be promoted, and the microbial balance of the skin surface can be maintained, so that the skin health state can be better and effectively maintained. Polylactic acid-glycolic acid copolymer (PLGA) is formed by random polymerization of two monomers, namely lactic acid and glycolic acid, is a degradable functional polymer organic compound, has good biocompatibility, no toxicity and good vesicle forming and film forming performances, and is widely applied to the fields of pharmacy, medical engineering materials and modern industry. Chitosan is a natural polysaccharide with good biocompatibility and biodegradability, so that the chitosan is widely applied in the field of biomedicine. The chitosan microsphere is a common chitosan product, has a large specific surface area and good biocompatibility, and can be used in the fields of medicine slow release, cell culture, biosensing and the like. Disclosure of Invention The invention provides a preparation method of a natural antibacterial neutral agent based on a PLGA slow release mechanism, which is used for skin bacteriostasis and other slow release bacteriostasis applications. In order to solve the technical problems, the invention provides the following technical scheme that the preparation method of the natural antibacterial neutral agent based on the PLGA slow release mechanism comprises the following specific steps: Firstly, taking polylactic acid PLGA as a carrier, weighing polylactic acid PLGA, dissolving in an organic solvent, then weighing meloxicam, adding into the solution, stirring and dissolving to form an oil phase, dripping the oil phase into a dispersing agent at a constant speed by a medical injector in a magnetic stirring state, volatilizing the organic solvent at normal temperature to solidify the microspheres, washing the obtained microsphere suspension, centrifuging, and freeze-drying the centrifuged microspheres to obtain meloxicam polylactic acid microspheres; Step two, adding chitosan powder into acetic acid solution, and stirring until the chitosan powder is completely dissolved to obtain chitosan solution; step three, adding the cross-linking agent into water, and stirring until the cross-linking agent is completely dissolved to obtain a cross-linking agent solution; Mixing meloxicam polylactic acid microspheres, chitosan solution and cross-linking agent solution, adding a surfactant, stirring to form uniform emulsion, adding the emulsion into a hardening agent, stirring until the cross-linking reaction is completed to obtain chitosan microspheres, washing the chitosan microspheres with deionized water to remove the surfactant and the cross-linking agent remained on the surface, and drying the chitosan microspheres in a ventilated drying chamber to obtain the natural antibacterial neutral agent based on PLGA slow-release mechanism. According to the technical scheme, in the first step, the organic solvent is a mixture of dichloromethane and acetone. According to the technical scheme, the dispersing agent in the first step is a polyvinyl alcohol solution. According to the technical scheme, the meloxicam polylactic acid microspheres in the first step are stored at 4 ℃. According to the technical scheme, in the fourth step, the cross-linking agent is ammonium sulfate. According to the technical scheme, the surfactant in the fourth step is Tween-80. Compared with the prior art, the invention has the following beneficial effects: The preparation method combines the advantages of polylactic acid-glycolic acid copolymer (PLGA) and chitosan, the PLGA has good biocompatibility and film forming property, and the chitosan is natural polysaccharide with excellent biocompatibility and biodegradability. This combination results in a final product that not only has excellent physical and chemical properties, but also has good biological activity. The slow release effect of the medicine can be realized by using PLGA as a carrier to prepare the meloxicam polylactic acid microsphere. This helps the sustained release of the drug at the skin surface, thereby maintaining the balance of skin pH for a longer period of time, promoting repair of skin barrier and maintenance of microbial balance. The chitosan has natural antibac