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CN-122005433-A - Moxa slice nanoparticle hydrogel and preparation method and application thereof

CN122005433ACN 122005433 ACN122005433 ACN 122005433ACN-122005433-A

Abstract

The invention relates to the technical field of medicines, in particular to a blumea balsamifera tablet nanoparticle hydrogel and a preparation method and application thereof. The blumea balsamifera tablet nanoparticle hydrogel is prepared from blumea balsamifera tablet nanoparticle dispersion liquid composed of L-borneol, tween-80, glycerol and ultrapure water and hydrogel matrix composed of soapberry saponin, pueraria polysaccharide, konjak glucomannan and ultrapure water. The invention successfully constructs the blumea balsamifera tablet nanoparticle hydrogel with good physicochemical properties, biological safety and multifunctional activity, and the hydrogel can obviously improve the healing disorder of the diabetic wound surface through the multi-target and multi-path synergistic effect, and the curative effect is obviously better than that of a clinical common control medicament. The blumea balsamifera tablet nanoparticle hydrogel provided by the invention has good conversion application prospect, and provides a new strategy and experimental basis for clinical treatment of chronic wounds of diabetes.

Inventors

  • PANG YUXIN
  • ZHU YUE
  • CHEN SU
  • WANG ZUHUA

Assignees

  • 贵州中医药大学

Dates

Publication Date
20260512
Application Date
20260116

Claims (10)

  1. 1. The blumea balsamifera nanoparticle hydrogel is characterized by being prepared from blumea balsamifera nanoparticle dispersion liquid and a hydrogel matrix, wherein: The blumea balsamifera tablet nanoparticle dispersion liquid consists of 0.83-5 mg/mL of L-borneol, 1-10% of tween-80 and 1% of glycerol in percentage by mass, and the solvent is ultrapure water; The hydrogel matrix consists of 1-2% of soapberry saponin, 2.5-10% of pueraria polysaccharide and 1-2% of konjac glucomannan in percentage by mass, and the solvent is ultrapure water.
  2. 2. The blumea balsamifera nanoparticle hydrogel of claim 1, wherein: The blumea balsamifera tablet nanoparticle dispersion liquid consists of 2.50-3.33 mg/mL of L-borneol, 1-3% of tween-80 and 1% of glycerol in percentage by mass, and the solvent is ultrapure water; the hydrogel matrix consists of 1.5-2% of soapberry saponin, 2.5-5% of pueraria polysaccharide and 1.5-2% of konjak glucomannan by mass percentage, and the solvent is ultrapure water.
  3. 3. The blumea balsamifera nanoparticle hydrogel of claim 2, wherein: the blumea balsamifera tablet nanoparticle dispersion liquid consists of 3.33 mg/mL of L-borneol, 1% of tween-80 and 1% of glycerol in percentage by mass, and the solvent is ultrapure water; the hydrogel matrix consists of 2% of soapberry saponin, 2.5% of pueraria polysaccharide and 2% of konjak glucomannan in percentage by mass, and the solvent is ultrapure water.
  4. 4. The method for preparing the blumea balsamifera nanoparticle hydrogel according to claim 1, which comprises the following steps: s1, preparing a moxa slice nanoparticle dispersion liquid: preparing an oil phase, namely precisely weighing L-borneol, dissolving in dichloromethane, placing in a water bath at 25+/-2 ℃, and carrying out ultrasonic treatment to obtain a clear and transparent oil phase solution; Preparing water phase, namely sequentially adding ultrapure water, tween-80 and glycerin into an aseptic container, and fully stirring to obtain uniform water phase solution; Emulsifying and volatilizing, namely dropwise adding the oil phase solution into a continuously stirred water phase solution at a slow and constant speed, and keeping the system continuously stirred at 25+/-2 ℃ after the dropwise adding is finished, so that dichloromethane volatilizes completely, thus obtaining the moxa slice nanoparticle dispersion liquid; s2, preparing the blumea balsamifera tablet nanoparticle hydrogel: Mixing the obtained tablet nanoparticle dispersion with ultrapure water, adding soapberry saponin, stirring at room temperature until soapberry saponin is completely dissolved, adding radix Puerariae polysaccharide, stirring at the same rotation speed until radix Puerariae polysaccharide is completely dissolved, uniformly spreading the glucomannan slowly and uniformly under continuous stirring, and stirring to obtain tablet nanoparticle hydrogel.
  5. 5. The method of claim 4, wherein the stirring speed in step S1 or step S2 is 300-500 rpm.
  6. 6. The method of claim 4, wherein the ultrasonic power in the preparation of the oil phase in step S1 is 300W and the ultrasonic time is 5 min.
  7. 7. The method of claim 4, wherein the stirring time in the aqueous phase preparation in step S1 is 5-10 min.
  8. 8. The method for preparing a hydrogel according to claim 4, wherein in the emulsification and volatilization step S1, the dripping rate of the oil phase solution is 1-2 drops/S, and the stirring time is 1 h after the dripping is completed.
  9. 9. The method for preparing the blumea balsamifera tablet nanoparticle hydrogel according to claim 4, wherein in the step S2, the mixing volume ratio of the blumea balsamifera tablet nanoparticle dispersion liquid and the ultrapure water is 1:4, the stirring time after the soapberry saponin is added is 15-20 min, the stirring time after the pueraria polysaccharide is added is 10-15min, and the stirring time after the konjak glucomannan is added is 20-30 min.
  10. 10. The use of the blumea balsamifera nanoparticle hydrogel of claim 1 in the preparation of a medicament for promoting the healing of diabetic wounds.

Description

Moxa slice nanoparticle hydrogel and preparation method and application thereof Technical Field The invention relates to the technical field of medicines, in particular to a blumea balsamifera tablet nanoparticle hydrogel and a preparation method and application thereof. Background Diabetic wound healing disorder is a major challenge facing the global medical and health system, and as the prevalence of diabetes continuously rises, the prevalence of diabetes rises year by year, and the diabetes becomes a primary cause of non-traumatic amputation. Unlike acute wounds, diabetic wounds have unique pathophysiological characteristics in that, driven by sustained hyperglycemia, a series of pathological cascades can be initiated, including chronic inflammation, excessive accumulation of Reactive Oxygen Species (ROS), impaired angiogenesis, neuropathy, and abnormal extracellular matrix (ECM) remodeling. These pathogenic factors interweave with each other to form a self-strengthening malignant cycle, so that the wound healing process is stopped at the inflammation stage, and finally the chronic refractory wound is developed. At present, clinical management of diabetic wounds mainly relies on multidisciplinary comprehensive treatment, including strict glycemic control, periodic surgical debridement, revascularization and application of functional dressings. However, these traditional treatments have focused on symptomatic relief and supportive care, and it is difficult to radically reverse the unbalanced state of the wound microenvironment. In recent years, with intensive research into regenerative medicine and immunology, new therapeutic methods are emerging. Among them, immunomodulation methods show great potential therapeutic prospects, such as wound immune microenvironment remodeling by strategies of modulating macrophage polarization, promoting regulatory T cell (Treg) recruitment, and the like. However, these leading edge therapies still face key bottlenecks such as low delivery efficiency, insufficient bioavailability, potential off-target toxicity, and the like, severely restricting the clinical transformation process. In various innovative dressing designs, hydrogel has become an ideal platform for treating diabetic wounds by virtue of unique physicochemical properties and biological functions. The high-performance hydrogel dressing not only can maintain the moist environment of the wound surface and regulate and control exudates, but also can be used as a multifunctional carrier to realize the controllable release of therapeutic molecules. The stimulus-responsive intelligent hydrogel is worthy of being concerned, and can dynamically respond to specific biomarkers (such as ROS and matrix metalloproteinase) in the diabetes wound microenvironment, so that the drug is released as required, and the treatment accuracy is remarkably improved. However, the existing hydrogel system still has inherent limitations that the synthesized polymer-based hydrogel possibly causes the problems of biocompatibility and safety of degradation products, and the natural polymer hydrogel often has the defects of weak mechanical property, limited drug carrying efficiency and the like, so that the complex pathological features of the diabetic wound surface are difficult to cooperatively cope with. Based on the method, an innovative moxa slice nanoparticle-hydrogel integrated system is developed through reasonable molecular design and material assembly strategies, so that the problems in the prior art are solved, a novel strategy with a great transformation prospect is provided for the treatment of the diabetic wound surface, and an innovative paradigm is also provided for the efficient delivery and functional integration of natural bioactive components. Disclosure of Invention The invention aims to provide a blumea balsamifera tablet nanoparticle hydrogel and a preparation method thereof. The invention also aims to provide the application of the blumea balsamifera tablet nanoparticle hydrogel in preparing a medicament for promoting the healing of diabetic wounds. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The moxa slice nanoparticle hydrogel is prepared from a moxa slice nanoparticle dispersion liquid and a hydrogel matrix, wherein the moxa slice nanoparticle dispersion liquid consists of 0.83-5mg/mL of L-borneol, 1-10% of tween-80 and 1% of glycerin by mass percent, the solvent is ultrapure water, the hydrogel matrix consists of 1-2% of soapberry saponin by mass percent, 2.5-10% of pueraria polysaccharide and 1-2% of konjak glucomannan by mass percent, and the solvent is ultrapure water. Preferably, in the blumea balsamifera tablet nanoparticle hydrogel, the blumea balsamifera tablet nanoparticle dispersion liquid consists of 2.50-3.33mg/mL of L-borneol, 1-3% of tween-80 and 1% of glycerin by mass percent, the solvent is ultrapure water, the hydrogel matrix consists of 1.5-2% of