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CN-122005439-A - HPV blocking gel based on synergistic charge trapping and calcium ion shielding and preparation method thereof

CN122005439ACN 122005439 ACN122005439 ACN 122005439ACN-122005439-A

Abstract

The invention provides HPV blocking gel based on synergistic charge trapping and calcium ion shielding and a preparation method thereof, and belongs to the technical field of biomedical materials. The gel of the present invention comprises sodium alginate, hydroxyethyl cellulose, glycerol, propylene glycol, methylparaben, sodium citrate, ethylhexyl glycerol and water. The gel provided by the invention does not need to rely on chemical antiviral drugs, realizes high-efficiency HPV blocking (target blocking rate is more than 95%) through a physical mechanism, maintains structural integrity and functionality in a body fluid environment with high calcium ion concentration, has excellent biocompatibility, and has no irritation to mucosal tissues.

Inventors

  • LI DONGSHUANG
  • QIN CUILIN

Assignees

  • 桂林清研皓隆新材料有限公司

Dates

Publication Date
20260512
Application Date
20260225

Claims (8)

  1. 1. HPV blocking gel based on synergistic charge trapping and calcium ion shielding, characterized by comprising the following components in percentage by mass: 2.0-2.3% of sodium alginate, 1.0-1.1% of hydroxyethyl cellulose, 9.5-10.5% of glycerol, 4.8-5.2% of propylene glycol, 0.18-0.20% of methylparaben, 0.05-0.1% of sodium citrate, 0.02-0.05% of ethylhexyl glycerol and the balance of water.
  2. 2. The HPV blocking gel based on synergistic charge trapping and calcium ion shielding of claim 1, comprising the following components in mass percent: sodium alginate 2.0%, hydroxyethyl cellulose 1.05%, glycerin 10.0%, propylene glycol 5.0%, methylparaben 0.19%, sodium citrate 0.08%, ethylhexyl glycerin 0.03%, and the balance water.
  3. 3. The HPV blocking gel based on synergistic charge trapping and calcium ion shielding according to claim 1 or 2, wherein the molecular weight of sodium alginate is 80-140 kda and the viscosity of the hydroxyethyl cellulose is 3500±500 mPa-s.
  4. 4. The HPV blocking gel based on synergistic charge trapping and calcium ion shielding of claim 1, wherein the mass ratio of glycerin to propylene glycol is 1.9-2.1:1.
  5. 5. The HPV blocking gel based on synergistic charge trapping and calcium ion shielding of claim 1, wherein the mass ratio of sodium alginate to sodium citrate is 20-25:1.
  6. 6. The method for preparing HPV blocking gel based on synergistic charge trapping and calcium ion screening according to any one of claims 1 to 5, characterized by the following steps: Heating and dissolving methylparaben, sequentially adding sodium citrate, glycerol and propylene glycol, cooling to room temperature, adding sodium alginate and hydroxyethyl cellulose under vacuum, homogenizing under high pressure, adding ethylhexyl glycerol, adding water to specified amount, packaging, and sterilizing.
  7. 7. The preparation method of the glass fiber reinforced plastic composite material according to claim 6, wherein the heating and dissolving temperature is 40-45 ℃, and the vacuum degree of vacuum conditions is-0.09-0.06 MPa.
  8. 8. The method according to claim 7, wherein the high-pressure homogenizing treatment is carried out at a speed of 2500 to 3000rpm for a treatment time of 10 to 30 minutes.

Description

HPV blocking gel based on synergistic charge trapping and calcium ion shielding and preparation method thereof Technical Field The invention relates to the technical field of biomedical materials, in particular to HPV blocking gel based on synergistic charge trapping and calcium ion shielding and a preparation method thereof. Background HPV infection is the main causative agent of cervical cancer, and more than 50 thousands of new cases are increased annually worldwide. At present, the clinic mainly depends on vaccine prevention and antiviral drug treatment, but has obvious limitations that the vaccine is ineffective for infected people, antiviral drugs (such as interferon) are easy to cause adverse reactions such as mucosal irritation and pain, the plexiform property of patients is poor, and drug resistance can be caused by long-term use. Recent studies have found that physical barrier methods can be used as an alternative. CN114588402a discloses an alginate gynecological gel, but its stability in high calcium ion environment (concentration of Ca 2+ in vaginal fluid 1.2-2.0 mM) is poor, HPV blocking rate is less than 80%. WO2023187422A1 adopts a chemically modified cellulose material, which improves the stability, but has the problems of complex preparation process, high cost and low virus adsorption efficiency. Clinical data indicate that cervical LEEP post-operative HPV persistent infection rate >30% for 6 months. Therefore, aiming at the prior art, how to obtain HPV blocking gel based on synergistic charge trapping and calcium ion shielding and a preparation method thereof is a technical problem to be solved at present. Disclosure of Invention The invention aims to provide HPV blocking gel based on synergistic charge trapping and calcium ion shielding and a preparation method thereof, which are used for solving the technical problems. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides HPV blocking gel based on synergistic charge trapping and calcium ion shielding, which comprises the following components in percentage by mass: 2.0-2.3% of sodium alginate, 1.0-1.1% of hydroxyethyl cellulose, 9.5-10.5% of glycerol, 4.8-5.2% of propylene glycol, 0.18-0.20% of methylparaben, 0.05-0.1% of sodium citrate, 0.02-0.05% of ethylhexyl glycerol and the balance of water. Further, the composition comprises the following components in percentage by mass: sodium alginate 2.0%, hydroxyethyl cellulose 1.05%, glycerin 10.0%, propylene glycol 5.0%, methylparaben 0.19%, sodium citrate 0.08%, ethylhexyl glycerin 0.03%, and the balance water. Further, the molecular weight of the sodium alginate is 80-140 kDa, and the viscosity of the hydroxyethyl cellulose is 3500+/-500 mPa.s. Further, the mass ratio of the glycerol to the propylene glycol is 1.9-2.1:1. Further, the mass ratio of the sodium alginate to the sodium citrate is 20-25:1. The invention also provides a preparation method of HPV blocking gel based on synergistic charge trapping and calcium ion shielding, comprising the following steps: Heating and dissolving methylparaben, sequentially adding sodium citrate, glycerol and propylene glycol, cooling to room temperature, adding sodium alginate and hydroxyethyl cellulose under vacuum, homogenizing under high pressure, adding ethylhexyl glycerol, adding water to specified amount, packaging, and sterilizing. Further, the temperature of the heating dissolution is 40-45 ℃, and the vacuum degree of the vacuum condition is-0.09-0.06 MPa. Further, the speed of the high-pressure homogenizing treatment is 2500-3000 rpm, and the treatment time is 10-30 min. The invention has the beneficial effects that: the invention relates to HPV blocking gel working through a dual mechanism of 'synergistic charge' and 'calcium ion shielding'. The gel has the following characteristics: the method has the advantages of no need of depending on chemical antiviral drugs, realization of high-efficiency HPV blocking (target blocking rate is more than 95%) through a physical mechanism, maintenance of structural integrity and functionality in a body fluid environment with high calcium ion concentration, excellent biocompatibility, no irritation to mucous membrane tissues, promotion of wound healing, reduction of postoperative HPV infection rate, simple and stable preparation process, and suitability for industrial mass production. The advantages of the HPV blocking gel of the present invention are also represented by the following: 1. Breaking through blocking efficiency: the blocking rate of HPV-16 is 97.3%, which is 23.2% higher than that of optimal control, and the blocking rate of HPV-18 is 96.8%, which is 21.5%. 2. Stability is significantly improved: The viscosity change in the high-calcium environment is only +2.8%, and the blocking rate is kept at 97.1%. 3. Excellent biocompatibility: The mucosa stimulation score is 0, and the cell survival rate is more than or equal to 9