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CN-122005442-A - Mechanical multifunctional hydrogel with eight-arm cage nanostructure for wound

CN122005442ACN 122005442 ACN122005442 ACN 122005442ACN-122005442-A

Abstract

The invention relates to a mechanical multifunctional hydrogel with an eight-arm cage nano structure for a wound and a preparation method thereof. The hydrogel network has a mechanical regulation function, is formed by photopolymerization of methacryloylated gelatin, octavinyl-cage polysilsesquioxane and N-isopropyl acrylamide, has a simple process, and is easy to prepare in a large scale. The hydrogel has ideal adhesiveness, photothermal conversion, biocompatibility and wound healing promotion. The purple cabbage anthocyanin is used as a natural visual probe, and can monitor the wound microenvironment in real time. The nano-cage crosslinked network is used as a rigid frame, so that the excessive expansion of the biopolymer hydrogel matrix can be limited, and the function damage and wound self-contraction blocking of the dressing caused by liquid absorption swelling are avoided. When the hydrogel is covered on skin wounds, the hydrogel can pull the wounds to shrink towards the center to promote wound healing, and continuously release resveratrol nano vesicles, so that regeneration and vascularization of wound tissues are promoted, and excessive inflammatory reaction is relieved.

Inventors

  • YAO SHUN
  • Luo Zidan
  • YANG SHIHAN

Assignees

  • 四川大学

Dates

Publication Date
20260512
Application Date
20260313

Claims (5)

  1. 1. The mechanical multifunctional hydrogel with the eight-arm cage nano structure for the wound is characterized by comprising the following specific preparation steps: (1) Preparing 100mg of methacryloylated gelatin into 10wt% aqueous solution, adding 2.5mg of 2-hydroxy-4' - (2-hydroxyethoxy) -2-methyl propiophenone into the aqueous solution, heating and dissolving, adding 1-5 mg of octavinyl-cage polysilsesquioxane, 50-300 mg of N-isopropyl acrylamide and 2-10 mg of dopamine hydrochloride, heating and stirring until the mixture is completely dissolved; (2) Adding 0.1-0.5wt% of purple cabbage anthocyanin probe and 5-10wt% of resveratrol nano-vesicles into the mixture solution in the step (1), and stirring to obtain uniform and transparent pre-gel solution; (3) And (3) irradiating the pre-gel solution with 365nm ultraviolet light of 6W for 4-8 min at room temperature, and obtaining the mechanical multifunctional hydrogel with the eight-arm cage nano structure through polymerization reaction.
  2. 2. The mechanical multifunctional hydrogel with the eight-arm cage nano structure for the wound, which is disclosed in claim 1, is characterized in that the methacryloylated gelatin in the mechanical multifunctional hydrogel component is prepared by mixing methacrylic anhydride and gelatin, the molecular weight of the methacryloylated gelatin is 8-14 kDa, the methacryloylated gelatin is used as a hydrogel matrix, 2-hydroxy-4' - (2-hydroxyethoxy) -2-methylpropenon is used as a photoinitiator, the octavinyl-cage polysilsesquioxane is used for constructing a controllable crosslinked network in a hydrogel system, N-isopropylacrylamide helps the hydrogel to realize hydrophilic-hydrophobic transformation under the heat induction, and dopamine is used for endowing the hydrogel with photo-thermal transformation capability and enhancing the tensile mechanical property of the hydrogel.
  3. 3. The mechanical multifunctional hydrogel with eight-arm cage nano structure for a wound of claim 1, wherein the purple cabbage anthocyanin probe in the mechanical multifunctional hydrogel component is prepared by extracting dry purple cabbage anthocyanin powder with 70% ethanol and freeze-drying the dried purple cabbage anthocyanin probe so as to realize visual monitoring of pH value of a wound microenvironment.
  4. 4. The mechanical multifunctional hydrogel with eight-arm cage nano structure for wounds, according to claim 1, wherein resveratrol nano vesicles in the mechanical multifunctional hydrogel component are prepared from soybean lecithin and resveratrol by a ultrasonic emulsification method, and can be continuously released as active ingredients.
  5. 5. A mechanical multifunctional hydrogel with eight-arm cage nano-structure for a wound according to claim 1, wherein the mechanical multifunctional hydrogel is covered at the wound in use, is lightly pressed to achieve adhesion, is irradiated by a 808nm near infrared light source for 2min, and is removed after the completion of the irradiation.

Description

Mechanical multifunctional hydrogel with eight-arm cage nanostructure for wound Technical Field The invention belongs to the technical field of biomedical materials, and particularly relates to a mechanical multifunctional hydrogel which combines multiple components and has an eight-arm cage nano structure and a preparation method thereof. Background Skin, as the primary interface with the external environment, is susceptible to thermal injury (e.g., burns), mechanical trauma (e.g., accidents), and chronic diseases (including diabetes and vascular diseases). Timely wound care is critical to accelerate the healing process and reduce the psychological burden on the patient after injury occurs. If not timely cared, these wounds are extremely susceptible to microbial invasion leading to infection, greatly delaying the healing process. Traditional clinical wound management relies primarily on gauze, cotton and bandages to encapsulate or compress the sutured wound. While these methods are cost effective and easy to use, they rely heavily on clinical experience in terms of frequent replacement and sterilization. Frequent inspection and changing of the dressing may increase the risk of recurrent infections and secondary damage to newly formed skin tissue. In recent years, the awareness of wound management has changed tremendously, from a dry wound environment to a properly moist environment, which is more beneficial to improving the quality and speed of wound healing. Therefore, a novel multifunctional material which can monitor the wound surface in real time, actively promote the wound surface to heal and accelerate the whole healing process is urgently needed. Methacryloylated gelatin is a semisynthetic photo-crosslinked gelatin polymer obtained by modification of amine groups in gelatin with methacrylic anhydride. The presence of methacryloyl groups causes the methacryloyl gelatin to initiate free radical polymerization in the presence of a photoinitiator, forming covalent bonds between the polymer chains, eventually forming a crosslinked hydrogel network. Although modified with methacrylic anhydride, the biocompatibility and biodegradability are not affected. Furthermore, the methacrylated gelatin still retains the arginine-glycine-aspartic acid peptide sequence in gelatin and the target sequence of matrix metalloproteinases, which can provide more cell attachment sites, thereby promoting tissue integration and regeneration. Meanwhile, as a partial hydrolysate of collagen, gelatin-based hydrogels may participate in collagen deposition during the healing process. Accordingly, a number of methacryloylated gelatin hydrogel dressing systems have been developed for the treatment of soft tissue injuries. Currently, numerous gelatin-based hydrogels have been developed to eliminate wound inflammatory factors or kill bacteria to restore the normal healing process, while promoting the activity of fibroblasts, keratinocytes and endothelial cells by delivering bioactive factors (such as growth factors, oxygen and cells), thereby achieving collagen deposition, re-epithelialization and revascularization. However, most of the gelatin-based hydrogels are based on biochemical methods to regulate the wound microenvironment, and although the gelatin-based hydrogels can effectively promote cell proliferation or inhibit infection, the gelatin-based hydrogels are difficult to directly cope with the key physical challenge of wound closure. The difficulty with wound closure is that it is necessary to overcome tissue retraction, sustained tension and difficulty in maintaining apposition accuracy, and improper closure can result in delayed healing, scarring and even recracking. Therefore, development of hydrogel materials capable of actively providing mechanical force to physically reduce the wound area while improving the microenvironment through biochemical means is essential for achieving accelerated wound healing in a real sense. The embryo wound healing can realize perfect regeneration of fetal skin, and provides a new bionic thought for wound dressing design. The key to this process is the actin cord formed inside the wound edge cells, and the pulling force generated by the contraction of the actin cord can actively pull the wound edge together in a 'wallet closing-in' manner. This is essentially a biomechanical strategy for promoting wound contraction by actively applying mechanical forces that can regulate the mechanical microenvironment of the wound, further promoting cell proliferation, collagen deposition and angiogenesis. At the same time, the fibroblast membrane surface receptor recognizes externally applied mechanical stress, causing the fibroblasts to activate and differentiate into myofibroblasts, further accelerating wound closure. The successful application of negative pressure wound therapy technology and recent research progress in mechanically driven muscle regeneration have motivated the design search for active ge