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CN-122005452-A - Oyster enzymolysis ultrafiltration extract nasal spray and preparation method thereof

CN122005452ACN 122005452 ACN122005452 ACN 122005452ACN-122005452-A

Abstract

The invention discloses a oyster enzymolysis ultrafiltration extract nasal spray and a preparation method thereof, wherein the nasal spray comprises the following components of oyster extract, preservative, sodium chloride and emulsifier, wherein the oyster extract contains 5-10wt%, the preservative contains 0.1-0.3wt%, the sodium chloride contains 0.05-0.15wt%, the emulsifier contains 0.1-0.3wt%, the pH=5.5-6.5, the preservative is one of benzalkonium chloride, sorbic acid and potassium sorbate, and the emulsifier is polysorbate 80. The invention improves the bioavailability of active ingredients, optimizes the flavor of the product, realizes the effect of safely and efficiently improving sleep through definite raw material composition, step-by-step process and quality control, solves the problems of low bioavailability, poor flavor and hidden safety hazards of the existing sleep improving products, has strong process repeatability, and is suitable for long-term use of chronic insomnia people.

Inventors

  • SUN JIAN

Assignees

  • 广州韶苑生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260119

Claims (10)

  1. 1. The oyster enzymolysis ultrafiltration extract nasal spray is characterized by comprising the following components of oyster extract, preservative, sodium chloride and emulsifier, wherein the oyster extract accounts for 5-10wt%, the preservative accounts for 0.1-0.3wt%, the sodium chloride accounts for 0.05-0.15wt%, and the emulsifier accounts for 0.1-0.3wt%.
  2. 2. The oyster enzymolysis ultrafiltration extract nasal spray according to claim 1, wherein the preservative is one of benzalkonium chloride, sorbic acid and potassium sorbate, and the emulsifier is polysorbate 80.
  3. 3. The oyster enzymatic hydrolysis ultrafiltration extract nasal spray according to claim 1, wherein the nasal spray comprises the following raw materials of oyster extract, potassium sorbate, sodium chloride and polysorbate 80, wherein the content of the oyster extract is 5-10wt%, the content of the potassium sorbate is 0.2wt%, the content of the sodium chloride is 0.1wt%, and the content of the polysorbate 80 is 0.2wt%.
  4. 4. A method for preparing the oyster enzymolysis ultrafiltration extract nasal spray according to any one of claims 1 to 3, characterized in that the method comprises the following steps: step 1, oyster homogenate enzymolysis: taking oyster as a raw material, and performing enzymolysis treatment to obtain an oyster enzymolysis product; Step2, ultrafiltration separation and purification: Ultrafiltration separation is carried out on the oyster enzymolysis product to obtain oyster extract; Step 3, deodorization: deodorizing the ultrafiltered oyster extract; Step 4, spray drying: concentrating the deodorized enzymolysis liquid until the solid concentration is 10-50wt%, and spray drying to obtain powder; Step 5, preparing and sterilizing a nasal spray: Step 5-1, purifying the dry powder by adopting an alcohol precipitation method, adding a preservative, sodium chloride and an emulsifier, and adjusting the pH value to 5.5-6.5; and 5-2, sterilizing.
  5. 5. The preparation method of oyster enzymolysis ultrafiltration extract nasal spray according to claim 4, wherein the specific steps of the step 1 are as follows: step 1-1, taking fresh oyster meat, draining, homogenizing, adjusting the pH to 6.0-6.8, adding protease according to a feed-liquid ratio of 1:2-4, and performing enzymolysis in a 50-60 ℃ constant-temperature magnetic stirring water bath for 1-6 h; And (3) inactivating enzyme in a boiling water bath at the temperature of between 1 and 2 and 100 ℃ for 5 to 15 minutes, cooling, centrifuging and filtering to obtain an oyster enzymolysis product.
  6. 6. The method for preparing oyster enzymolysis ultrafiltration extract nasal spray according to claim 5, wherein the protease is bromelain or papain, and the enzyme adding amount is 500-700U/g.
  7. 7. The preparation method of oyster enzymolysis ultrafiltration extract nasal spray according to claim 4, wherein the specific steps of the step 2 are as follows: step 2-1, performing preliminary filtration on the enzymolysis product by adopting a 200 mu m ceramic membrane filtration system so as to remove macromolecular impurities; Step 2-2, carrying out fractional ultrafiltration through a 3kDa ultrafiltration membrane, and collecting components with the concentration of < 3 kDa; And 2-3, desalting by using ion exchange resin.
  8. 8. The method for preparing the oyster enzymolysis ultrafiltration extract nasal spray according to claim 4, wherein in the step 3, the deodorization mode is Maillard reaction or beta-cyclodextrin embedding method, the Maillard reaction is carried out under the process conditions of 80-120 ℃ for 10-50 min and pH=7-9, and the ratio of reducing sugar to amino acid is 1-1.5:1-1.5, and the reducing sugar is xylose, glucose or sucrose.
  9. 9. The method for preparing oyster enzymolysis ultrafiltration extract nasal spray according to claim 4, wherein in the step 4, the air inlet temperature of spray drying is 140-200 ℃ and the air outlet temperature is 70-100 ℃.
  10. 10. The method for preparing oyster enzymatic ultrafiltration extract nasal spray according to claim 4, wherein in step 5-2, the sterilization mode is irradiation sterilization or steam sterilization.

Description

Oyster enzymolysis ultrafiltration extract nasal spray and preparation method thereof Technical Field The invention belongs to the technical field of health care products, relates to a nasal spray, and in particular relates to a health care product for improving sleep, which takes oyster as a raw material and adopts a nasal spray formulation, and a preparation method thereof. Background With the acceleration of modern life pace and pressure increase, sleep disorders have become a common problem affecting human health. According to the related researches, about 30% of people worldwide have insomnia symptoms, about 3 hundred million people in China suffer from sleep disorders, and the sleep disorders are manifested by difficulty in falling asleep, poor sleep maintenance, low sleep quality and the like, and can lead to fatigue, attention loss, abnormal emotion and even increase the risk of cardiovascular diseases, diabetes and other chronic diseases for a long time. At present, means for improving sleep mainly comprise drug treatment, psychological treatment and health care product conditioning. In the drug treatment, while hypnotics such as benzodiazepines, bifenoxine antagonists and the like can relieve insomnia in a short period, tolerance and dependence are easy to generate after long-term use, side effects such as cognitive dysfunction, headache, somnolence and the like can be possibly caused, the efficacy on chronic insomnia is limited, and the cognitive behavior therapy is difficult to widely popularize as a first-line non-drug treatment means due to the problems of high cost, long time consumption, lack of professionals and the like. In the field of health care products, the existing products mainly take oral dosage forms, and although partial raw materials (such as plant extracts, amino acids and the like) are proved to have certain sleep-aiding potential, the problem of low bioavailability exists, such as the fact that protein polypeptide components are easily damaged by gastric juice and degraded by digestive tract enzymes after being taken orally, and the effective action is difficult. In addition, the problems of fishy smell, poor taste and the like of part of products exist due to the characteristics of raw materials, and the user acceptance is affected. Oyster is used as the first medicinal and edible substance in China, is rich in protein, amino acid (such as glycine and arginine), mineral substances (such as zinc, selenium and magnesium) and the like, and has the activities of improving sleep, resisting oxidation, resisting fatigue and the like, and the oyster is recorded in the traditional Chinese medicine in a blood nourishing and nerve soothing way. However, in the prior art, oyster development is mostly focused on oral preparations, the problems of low bioavailability of active ingredients, obvious fishy smell and the like of the oyster are not fully solved, and the effect of improving sleep is difficult to be maximally exerted. Therefore, development of a sleep improving product based on oyster raw materials, high in bioavailability, good in safety and suitable in flavor becomes an urgent need in the industry. Disclosure of Invention The invention provides a oyster enzymolysis ultrafiltration extract nasal spray and a preparation method thereof, aiming at solving the problems of tolerance, dependence and side effect existing in the existing sleep improving products in drug treatment, low bioavailability of oral health care products, poor flavor of part of products (such as obvious fishy smell of oyster products) and the like. The invention improves the bioavailability of active ingredients, optimizes the flavor of the product, realizes the effect of safely and efficiently improving sleep through definite raw material composition, step-by-step process and quality control, solves the problems of low bioavailability, poor flavor and hidden safety hazards of the existing sleep improving products, and has strong process repeatability. The invention aims at realizing the following technical scheme: The oyster enzymolysis ultrafiltration extract nasal spray comprises the following components of oyster extract, preservative, sodium chloride and emulsifier, wherein the oyster extract accounts for 5-10wt%, the preservative accounts for 0.1-0.3wt%, the sodium chloride accounts for 0.05-0.15wt%, the emulsifier accounts for 0.1-0.3wt%, the pH=5.5-6.5, the preservative is one of benzalkonium chloride, sorbic acid and potassium sorbate, and the emulsifier is polysorbate 80. A preparation method of the oyster enzymolysis ultrafiltration extract nasal spray comprises the following steps: step 1, oyster homogenate enzymolysis: The oyster enzymolysis product is obtained by taking oyster as a raw material and carrying out enzymolysis treatment, and the specific steps are as follows: Step 1-1, taking fresh oyster meat, draining, homogenizing, adjusting the pH to 6.0-6.8, adding protease according to a feed-li