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CN-122005454-A - Nitric oxide gas foaming agent and preparation method and application thereof

CN122005454ACN 122005454 ACN122005454 ACN 122005454ACN-122005454-A

Abstract

The invention discloses a nitric oxide gas foaming agent, a preparation method and application thereof, belonging to the technical field of biological composite materials, comprising gel foam, wherein the gel foam is semisolid gel formed by dispersing gas in a hydrogel framework in a micro-bubble mode; the gel foam prepared by the invention has the advantages of adjustable nitric oxide gas content, long foam maintenance time and high mechanical strength, can overcome the defects of the prior nitric oxide gas local treatment application, adopts auxiliary materials approved by the FDA, has the advantages of simple components and good safety, is simple and controllable in operation and easy to realize amplified production, has antibacterial, hemostatic, anti-inflammatory and immunoregulatory effects, and can be used for repairing wound surfaces of skin and mucous membranes (gastrointestinal tract and uterus) of cavities.

Inventors

  • XU HELIN
  • OuYang Shenyuan
  • JIA YIYING
  • WANG JIE

Assignees

  • 温州医科大学

Dates

Publication Date
20260512
Application Date
20260327

Claims (9)

  1. 1. A nitric oxide gas foaming agent comprises gel foam, and is characterized in that the gel foam is semisolid gel formed by dispersing gas in a hydrogel framework in a micro-bubble form; The gas consists of nitric oxide and nitrogen with the volume ratio of 1:10, and the hydrogel framework is a fibrous aggregate formed by compounding chitosan and sodium alginate solution.
  2. 2. The nitric oxide gas foaming agent according to claim 1, wherein the volume ratio of the hydrogel to the gas is 1:0.1-1:10; The hydrogel comprises 0.5% -5.0% of chitosan, 0.5% -5.0% of sodium alginate and water, the nitric oxide gel foaming agent contains nitric oxide with the concentration of 50-1000 mu mol/L, the volume ratio of the hydrogel to the gas is 1:0.5-1:5, the hydrogel comprises 1.0% -3.0% of chitosan, 1.0% -3.0% of sodium alginate and water, and the nitric oxide gel foaming agent contains nitric oxide with the concentration of 60-500 mu mol/L.
  3. 3. The preparation method of the nitric oxide gas foaming agent is characterized by comprising the following steps of: S1, preparing a chitosan solution, namely dissolving chitosan powder in an acidic aqueous solution, and stirring until the chitosan powder is completely dissolved; s2, preparing sodium alginate solution, namely dissolving sodium alginate powder in distilled water, and stirring until the sodium alginate powder is completely dissolved; s3, mixing, namely mixing the chitosan solution in the step S1 and the sodium alginate solution in the step S2 according to a certain volume ratio of 1:0.5-1:3, and uniformly stirring to prepare a viscous fibrous aggregate; And S4, foaming, namely filling the fibrous condensate solution obtained in the step S3 into a closed foaming bottle, connecting a three-way valve, pumping air in the bottle, then filling mixed high-pressure gas of nitric oxide and nitrogen into the bottle, maintaining the air pressure in the bottle at 100-600 psi, and severely shaking the foaming bottle to disperse the gas into the viscous fibrous condensate to prepare the gel foam.
  4. 4. The method for preparing a nitric oxide gas foam agent according to claim 3, wherein the chitosan solution and the sodium alginate solution in the step S3 are mixed according to a certain volume ratio of 1:0.5-1:3.
  5. 5. The method for preparing a nitric oxide gas foam agent according to claim 3, wherein the pressure of the foaming bottle is maintained at 200-400 psi.
  6. 6. The application of the nitric oxide gas foam agent is characterized in that the nitric oxide gas foam agent is prepared by the preparation method of any one of the nitric oxide gas foam agents in claims 3-5, and the nitric oxide gas gel foam agent is applied to skin diseases such as skin burn, scald or diabetic foot and the like to promote wound healing.
  7. 7. The application of the nitric oxide gas foam agent is characterized in that the nitric oxide gas foam agent is prepared by the preparation method of any one of the nitric oxide gas foam agents in claims 3-5, and the nitric oxide gas gel foam agent is applied to a therapeutic drug for treating gastritis, ulcerative colitis, enteritis and gynecological inflammation caused by helicobacter pylori.
  8. 8. The application of the nitric oxide gas foam agent, which is characterized in that the nitric oxide gas foam agent is prepared by the preparation method of the nitric oxide gas foam agent in any one of claims 3-5, and the nitric oxide gas gel foam agent is applied to the antibacterial medicament.
  9. 9. The application of the nitric oxide gas foam agent, which is characterized in that the nitric oxide gas foam agent is prepared by the preparation method of the nitric oxide gas foam agent in any one of claims 3-5, and the nitric oxide gas gel foam agent is applied to hemostatic materials.

Description

Nitric oxide gas foaming agent and preparation method and application thereof Technical Field The invention belongs to the technical field of biological composite materials, and particularly relates to a nitric oxide gas foaming agent and a preparation method and application thereof. Background Bacterial infection is a major cause of difficult repair of skin wounds. In recent years, the treatment of bacterial infection by nitric oxide (Nitric oxide, NO) gas has been widely studied and interesting, NO is a diatomic free radical, and can kill bacteria by causing lipid peroxidation, DNA cleavage, protein dysfunction and the like, and the drug resistance is not easy to generate. In addition, NO can react with free radical superoxide to form peroxynitrite anion (ONOO-) and nitrous oxide (N 2O3), which cause rupture of bacterial membrane and bacterial dysfunction, and thus NO exhibits excellent antibacterial activity against both gram positive and negative bacteria, even against drug-resistant bacteria. At present, an NO treatment system mainly comprises a nitric oxide gas inhalation, a nitric oxide donor and a nitric oxide phospholipid microbubble, wherein the nitric oxide gas inhalation treatment through the lung has the advantages of quick effect, simple administration and the like, but the problems of difficult accurate control of gas dosage, lung stimulation, quick gas removal, low target concentration of wound surfaces and the like exist, the nitric oxide donor needs to be converted into nitric oxide in vivo through chemical hydrolysis or enzyme catalysis, the nitric oxide donor has the advantages of controllable dosage and mild effect, and the nitric oxide phospholipid microbubble has the problems of low gas inclusion amount, gas solution leakage, difficulty in realizing large-scale production and the like. The foam is a dispersion system formed by dispersing gas in a continuous phase in a micro-bubble form, has the advantages of high gas content, easiness in preparation and the like by virtue of a unique physical structure, is prepared by mixing air with a surfactant solution by using a special device, is applied to the treatment of cosmetics or skin diseases, and has the problems of weak mechanical strength, easiness in flowing, easiness in breaking bubbles, short residence time and the like. Disclosure of Invention The invention aims to provide a nitric oxide gas foaming agent and a preparation method and application thereof, and aims to solve the problems that the traditional foam has the defects of weak mechanical strength, short half-life period, unadjustable gas concentration, no antibacterial activity, no wound repair activity and the like, and cannot meet the requirement of nitric oxide local treatment. In order to achieve the aim, the invention provides the technical scheme that the nitric oxide gas foaming agent comprises gel foam, wherein the gel foam is semisolid gel formed by dispersing gas in a hydrogel framework in a micro-bubble form; The gas consists of nitric oxide and nitrogen with the volume ratio of 1:10, and the hydrogel framework is a fibrous aggregate formed by compounding chitosan and sodium alginate solution. As a preferred embodiment, the volume ratio of the hydrogel to the gas is 1:0.1-1:10; As a preferred implementation mode, the hydrogel comprises 0.5% -5.0% of chitosan, 0.5% -5.0% of sodium alginate and water, the nitric oxide gel foaming agent comprises 50% -1000 mu mol/L of nitric oxide, the volume ratio of the hydrogel to the gas is 1:0.5% -1:5, the hydrogel comprises 1.0% -3.0% of chitosan, 1.0% -3.0% of sodium alginate and water, and the nitric oxide gel foaming agent comprises 60% -500 mu mol/L of nitric oxide. The invention also discloses a preparation method of the nitric oxide gas foaming agent, which comprises the following steps: S1, preparing a chitosan solution, namely dissolving chitosan powder in an acidic aqueous solution, and stirring until the chitosan powder is completely dissolved; s2, preparing sodium alginate solution, namely dissolving sodium alginate powder in distilled water, and stirring until the sodium alginate powder is completely dissolved; s3, mixing, namely mixing the chitosan solution in the step S1 and the sodium alginate solution in the step S2 according to a certain volume ratio of 1:0.5-1:3, and uniformly stirring to prepare a viscous fibrous aggregate; And S4, foaming, namely filling the fibrous condensate solution obtained in the step S3 into a closed foaming bottle, connecting a three-way valve, pumping air in the bottle, then filling mixed high-pressure gas of nitric oxide and nitrogen into the bottle, maintaining the air pressure in the bottle at 100-600 psi, and severely shaking the foaming bottle to disperse the gas into the viscous fibrous condensate to prepare the gel foam. In the step S3, the chitosan solution and the sodium alginate solution are mixed according to a certain volume ratio of 1:0.5-1:3. As a preferred embodiment,