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CN-122005458-A - Inulin-carboxymethyl chitosan modified urolithin A liposome and preparation method and application thereof

CN122005458ACN 122005458 ACN122005458 ACN 122005458ACN-122005458-A

Abstract

The invention provides an inulin-carboxymethyl chitosan modified urolithin A liposome and a preparation method and application thereof, wherein S1, urolithin A, egg yolk lecithin and beta-sitosterol are mixed and dissolved in absolute ethyl alcohol to obtain a mixed solution, the mixed solution is subjected to water bath spin evaporation until the ethyl alcohol is removed, a layer of oily film is formed in a container, tween-80 aqueous solution is added, stirring and heating are carried out until the raw liposome is completely hydrated to form a coarse liposome, the coarse liposome is subjected to ultrasonic and centrifugal treatment to obtain supernatant fluid which is urolithin A liposome, S2, carboxymethyl chitosan solution is added into urolithin A liposome, stirring is carried out uniformly to obtain urolithin A liposome modified by carboxymethyl chitosan, and S3, inulin solution is added into urolithin A liposome modified by carboxymethyl chitosan, stirring is carried out uniformly to obtain the inulin-carboxymethyl chitosan modified urolithin A liposome. The invention can obviously improve the encapsulation efficiency and the bioavailability of the urolithin A liposome.

Inventors

  • XIA XIAODONG
  • GU YUNJI
  • HONG HAO
  • ZHOU LIPING

Assignees

  • 大连星海湾实验室

Dates

Publication Date
20260512
Application Date
20260119

Claims (10)

  1. 1. The preparation method of the inulin-carboxymethyl chitosan modified urolithin A liposome is characterized by comprising the following steps: S1, mixing urolithin A, egg yolk lecithin and beta-sitosterol and dissolving in absolute ethyl alcohol to obtain a mixed solution; Performing water bath rotary evaporation on the mixed solution until ethanol is removed, observing that a layer of oily film is formed in the container, adding tween-80 water solution, stirring and heating until complete hydration is performed to form crude liposome; carrying out ultrasonic and centrifugal treatment on the crude liposome to obtain a supernatant fluid which is urolithin A liposome; S2, adding carboxymethyl chitosan solution into the urolithin A liposome, and uniformly stirring to obtain the urolithin A liposome modified by carboxymethyl chitosan; And S3, adding an inulin solution into the carboxymethyl chitosan modified urolithin A liposome, and uniformly stirring to obtain the inulin-carboxymethyl chitosan modified urolithin A liposome.
  2. 2. The preparation method according to claim 1, wherein in the step S1, the mass ratio of the urolithin A, the egg yolk lecithin and the beta-sitosterol is 1:10:2.
  3. 3. The preparation method according to claim 1, wherein in the step S1, the water bath spin steaming is performed at a temperature of 30-37 ℃ and a rotating speed of 50-80r/min.
  4. 4. The preparation method according to claim 1, wherein in the step S1, the concentration of the Tween-80 aqueous solution is 0.5-1.5%, and the amount of the Tween-80 aqueous solution is 500-1000 times the mass of the urolithin A.
  5. 5. The method according to claim 1, wherein the concentration of carboxymethyl chitosan solution in step S2 is 3-7mg/mL.
  6. 6. The method according to claim 1, wherein in step S2, the volume ratio of carboxymethyl chitosan solution to urolithin a liposome is 1:1.
  7. 7. The method according to claim 1, wherein in step S3, the concentration of the inulin solution is 3-7mg/mL.
  8. 8. The method according to claim 1, wherein in step S3, the volume ratio of inulin solution to carboxymethyl chitosan modified urolithin a liposome is 1:1.
  9. 9. An inulin-carboxymethyl chitosan modified urolithin a liposome prepared by the preparation method of any one of claims 1 to 8.
  10. 10. Use of an inulin-carboxymethyl chitosan modified urolithin a liposome according to claim 9 for the preparation of a medicament for the prevention of immune decline.

Description

Inulin-carboxymethyl chitosan modified urolithin A liposome and preparation method and application thereof Technical Field The invention relates to the technical field of urolithin A delivery, in particular to an inulin-carboxymethyl chitosan modified urolithin A liposome and a preparation method and application thereof. Background Urolithin A is one of metabolites produced by degradation of ellagitannins in living organisms by intestinal microorganisms, and current research shows that urolithin A has various biological activities, such as anti-inflammatory, anti-aging, anti-tumor and immunity regulation. However, the water-soluble polymer is not easily absorbed by human body due to its poor water solubility and low bioavailability, and its functional properties cannot be fully exerted, which limits its application in the fields of foods, medicines, etc. Liposomes are closed vesicles with a bilayer structure formed by self-assembly of amphipathic phospholipids dispersed in an aqueous phase. Compared with other delivery systems, the liposome closed vesicle structure can simultaneously embed the liposoluble substances and the water-soluble substances, so that the bioavailability and stability of the active substances are improved. The traditional liposome is not modified, is sensitive to the external environment, and is easy to aggregate to cause leakage of embedding substances. The liposome is modified by polysaccharide, so that the embedding rate, stability and bioavailability of the liposome can be effectively improved. How to prepare the liposome modified layer by layer, the encapsulation rate and the bioavailability of the urolithin A are improved, and the method has important significance for clinical application of the urolithin A. Disclosure of Invention Aiming at the problems existing in the prior art, the invention provides an inulin-carboxymethyl chitosan modified urolithin A liposome and a preparation method and application thereof. The invention can obviously improve the encapsulation efficiency and the bioavailability of the urolithin A liposome. The technical scheme of the invention is as follows: The first object of the invention is to provide a preparation method of inulin-carboxymethyl chitosan modified urolithin A liposome, which comprises the following steps: S1, mixing urolithin A, egg yolk lecithin and beta-sitosterol and dissolving in absolute ethyl alcohol to obtain a mixed solution; Performing water bath rotary evaporation on the mixed solution until ethanol is removed, observing that a layer of oily film is formed in the container, adding tween-80 water solution, stirring and heating until complete hydration is performed to form crude liposome; carrying out ultrasonic and centrifugal treatment on the crude liposome to obtain a supernatant fluid which is urolithin A liposome; S2, adding carboxymethyl chitosan solution into the urolithin A liposome, and uniformly stirring to obtain the urolithin A liposome modified by carboxymethyl chitosan; And S3, adding an inulin solution into the carboxymethyl chitosan modified urolithin A liposome, and uniformly stirring to obtain the inulin-carboxymethyl chitosan modified urolithin A liposome. In one embodiment of the invention, in step S1, the mass ratio of urolithin A, egg yolk lecithin and beta-sitosterol is 1:10:2. In one embodiment of the present invention, in step S1, after urolithin A, egg yolk lecithin and beta-sitosterol are mixed and dissolved in absolute ethanol, magnetic stirring is applied, and the speed of the magnetic stirring is 300r/min. In one embodiment of the invention, in the step S1, the condition of water bath rotary steaming is that the temperature is 30-37 ℃ and the rotating speed is 50-80r/min. In one embodiment of the invention, in the step S1, the concentration of the Tween-80 aqueous solution is 0.5-1.5%, and the dosage of the Tween-80 aqueous solution is 500-1000 times of the mass of the urolithin A. In one embodiment of the present invention, in step S1, the condition of stirring and heating is that the heating temperature is 55 ℃ and the stirring speed is 200r/min. In one embodiment of the present invention, in step S1, the ultrasonic treatment of the crude liposome is performed under ice bath conditions, the ultrasonic power is 180W, the ultrasonic treatment time is 10min, the centrifugal temperature is 4 ℃, the centrifugal rotational speed is 4000r/min, and the centrifugal time is 10min. In one embodiment of the present invention, in step S2, the concentration of the carboxymethyl chitosan solution is 3-7mg/mL. In one embodiment of the invention, in the step S2, the preparation method of the carboxymethyl chitosan solution comprises the steps of dissolving carboxymethyl chitosan in deionized water, and stirring for 1h under the condition of 300r/min to prepare the carboxymethyl chitosan solution with the concentration of 3-7 mg/mL. In one embodiment of the invention, in step S2, the volume ratio of carboxymethyl