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CN-122005462-A - Tannic acid powder containing metal-polyphenol nano enzyme and preparation method and application thereof

CN122005462ACN 122005462 ACN122005462 ACN 122005462ACN-122005462-A

Abstract

The invention discloses tannic acid powder containing metal-polyphenol nano enzyme, a preparation method and application thereof, relating to the technical field of medical biological materials, wherein the tannic acid powder consists of cerium-tannic acid nano enzyme, tannic acid, chitosan and corn starch according to a specific proportion; the preparation method comprises the steps of self-assembling tannic acid and cerium ions through coordination to form nano enzyme, and mixing the nano enzyme with other components to prepare powder. The powder utilizes the peroxidase-like catalytic activity of nano enzyme to generate sterilizing free radicals, realizes the efficient and difficultly drug-resistant antibacterial effect, has the anti-inflammatory and antioxidant properties of tannic acid and the hemostatic and healing promotion functions of chitosan, and has multiple effects of stopping bleeding, resisting bacteria, resisting inflammation and promoting wound healing. The invention has the advantages of easily obtained raw materials, simple process, low cost, good biocompatibility and high stability, is suitable for large-scale production, and has good application prospect in animal clinical wound treatment.

Inventors

  • ZHAO XIAOPING
  • ZHENG HAIXUE
  • ZHOU CHENGRU
  • ZHU ZIXIANG
  • TIAN HONG

Assignees

  • 兰州大学

Dates

Publication Date
20260512
Application Date
20260331

Claims (10)

  1. 1. The preparation method of the cerium-tannic acid nano enzyme is characterized by comprising the following steps of respectively dissolving tannic acid and cerium nitrate hexahydrate in deionized water according to a molar ratio of 1:6 to 1:800, dropwise adding cerium salt solution into the tannic acid solution under stirring, regulating pH of the mixed solution to 7.5-8.5 by using alkali solution, continuously stirring at room temperature for reaction for 2-4 hours, centrifuging, washing and vacuum freeze-drying the reaction solution, and obtaining dark brown cerium-tannic acid nano enzyme powder.
  2. 2. The cerium-tannic acid nanoenzyme according to claim 1, wherein the molar ratio of tannic acid to cerium nitrate hexahydrate is 1:100, the alkaline solution is a sodium hydroxide solution of 2M, the pH is adjusted to 8.0, the reaction time is 3 hours, the centrifugal rotation speed is 8000 rpm, and the washing solvent is deionized water.
  3. 3. A tannic acid compound powder containing metal-polyphenol nano enzyme is characterized by comprising, by mass, 10% -20% of cerium-tannic acid nano enzyme, 5% -20% of tannic acid, 5% -20% of chitosan and the balance of corn starch according to claim 1 or 2.
  4. 4. The tannic acid compound powder containing the metal-polyphenol nano enzyme according to claim 3, which is characterized by comprising, by mass, 10% of cerium-tannic acid nano enzyme, 20% of tannic acid, 5% of chitosan and 65% of corn starch.
  5. 5. The tannic acid compound powder containing metal-polyphenol nano-enzyme according to claim 3 or 4, wherein the molar ratio of tannic acid to cerium ions in the cerium-tannic acid nano-enzyme is 1:6 to 1:800, preferably 1:100.
  6. 6. A method for preparing the tannic acid compound powder containing metal-polyphenol nano-enzyme according to any one of claims 3 to 5, comprising the steps of: (1) The preparation of cerium-tannic acid nano enzyme comprises the steps of dissolving tannic acid and cerium salt in deionized water, mixing to form a reaction solution, regulating the pH value of the reaction solution to 7.5-8.5, stirring at room temperature for 2-4 hours, centrifuging, washing and freeze-drying after the reaction is finished to obtain cerium-tannic acid nano enzyme powder; (2) Raw material pretreatment, namely respectively crushing and sieving cerium-tannic acid nano enzyme powder, tannic acid, chitosan and corn starch obtained in the step (1); (3) Mixing and sub-packaging, namely weighing and uniformly mixing the pretreated components according to the mass percent of claim 3 or 4 to obtain compound powder, and sub-packaging.
  7. 7. The preparation method of the freeze-drying agent according to claim 6, wherein in the step (1), a sodium hydroxide solution is adopted for the pH adjustment, the pH value after the adjustment is 8.0, the stirring reaction time is 3 hours, the centrifugal rotating speed is 7000-9000 rpm, and the freeze-drying condition is that the cold trap temperature is-20 ℃ to-70 ℃ and the vacuum degree is lower than 20 Pa.
  8. 8. The preparation method of claim 6, wherein in the step (2), a 80-100 mesh screen is adopted for sieving, and in the step (3), the mixing time is not less than 30 minutes.
  9. 9. Use of the tannic acid compound powder comprising metal-polyphenol nanoenzyme of any of claims 3-5 in the manufacture of a medicament for treating a wound.
  10. 10. The use according to claim 9, wherein the wound is a skin or mucosal wound with bleeding, infection or inflammation, the treatment comprising haemostasis, antibacterial, anti-inflammatory and/or promoting wound healing, preferably the medicament is a topical powder formulation for use in clinical wound treatment of animals.

Description

Tannic acid powder containing metal-polyphenol nano enzyme and preparation method and application thereof Technical Field The invention belongs to the technical field of medical biological materials, and particularly relates to tannic acid powder containing metal-polyphenol nano-enzyme, and a preparation method and application thereof. Background Tannic Acid (TA) is a natural plant polyphenol which is widely used in trees and higher plants, can be extracted from galls, contains abundant phenolic hydroxyl groups in the molecular structure, and endows the galls with various biological activities such as antioxidation, anti-inflammatory, antibacterial, antivirus and the like. Tannic acid has received attention in the field of biomaterials for its good biocompatibility and diverse functional properties, showing potential in particular in wound dressings and topical therapeutic agents. In addition, tannic acid plays an important role in stabilizing nanoparticles, improving bioavailability of drugs and enhancing efficacy. However, tannic acid itself has a large molecular weight and strong hydrophilicity, and if used at too high a concentration in a topical application, it may cause irritation to mucosal tissues, affecting safety and tolerance in wound treatment. Meanwhile, tannic acid is easy to oxidize in a liquid environment, has poor stability, and limits long-term storage and practical application effects. Therefore, how to improve the release performance of tannic acid, reduce the local irritation and improve the stability of tannic acid becomes an important subject for promoting the clinical transformation. In recent years, self-assembly of metal ions and polyphenol compounds based on coordination bonds to form metal-polyphenol nano-complexes becomes an effective functional modification strategy. The nano complex not only has good biocompatibility and adjustable catalytic property, but also can realize efficient antibacterial effect at a lower concentration, thereby avoiding the problem of cytotoxicity caused by using high-concentration tannic acid. Of particular concern, such materials may exhibit catalytic activity similar to that of natural enzymes, known as "nanoenzymes," which destroy the bacterial environment by catalytic reaction, are not prone to induce bacteria to develop resistance, and provide a new approach to cope with the current severe antibiotic resistance problem. Among the numerous metal ions, cerium ion (Ce 3+/Ce4+) is favored in the construction of functional nanoenzymes due to its variable valence state and good biosafety. The cerium-tannic acid (CeTA) nano-network formed by self-assembly by using tannic acid and cerium nitrate as raw materials has various enzyme-like catalytic properties. Based on the transition between cerium ion valences, ceTA nanoezymes exhibit significant peroxidase-like (POD) activity, capable of catalyzing the decomposition of hydrogen peroxide (H 2O2) to generate hydroxyl radicals (·oh). The free radical has strong oxidizing ability, can efficiently destroy the integrity of bacterial cell membranes, oxidize intracellular proteins and nucleic acids, thereby inhibiting bacterial proliferation and even directly killing bacteria, and providing core efficacy support for the treatment of infectious wounds and resisting bacterial drug resistance. Although CeTA nanoezymes exhibit excellent antibacterial properties and catalytic activity in vitro, they still face a number of challenges in practical applications. Firstly, the stability of the nano-enzyme in a dry state and in a long-term storage process needs to be further improved, secondly, the pure nano-enzyme powder is not easy to uniformly disperse and attach at a wound part to influence the full play of the curative effect, and furthermore, the ideal wound therapeutic agent has multiple functions of stopping bleeding, resisting bacteria and inflammation and promoting healing, and the nano-enzyme with a single component is difficult to meet the comprehensive requirement. Therefore, the development of the external preparation which can combine the efficient catalytic antibacterial performance of the nano enzyme with other bioactive components, has good stability, convenient use and comprehensive treatment function has important practical significance. In the aspect of external dosage form selection, the powder has the advantages of large coverage area, easy storage and transportation, convenient use, capability of absorbing wound exudates and forming a protective layer, and the like, and is particularly suitable for treating skin, mucous membrane and irregular wound in animal clinic. At present, some wound powders based on chitosan, gelatin or other natural polymers have been reported, but they either focus on physical coverage and moisture retention, or have limited antibacterial efficacy, especially poor resistance to drug-resistant bacteria. CeTA nanometer enzyme with high-efficiency enzyme catalysis antibacterial