CN-122005466-A - Blood fat reducing diglyceride composition and preparation method and application thereof

Abstract

The invention discloses a blood fat reducing diglyceride composition, a preparation method and application thereof, and relates to the technical field of biological medicines, wherein the blood fat reducing diglyceride composition comprises 30-60% of diglyceride, 30-55% of structured matrix, 2-8% of phytosterol, 1-6% of auxiliary functional composition and the balance of water. The invention successfully constructs a novel water-dispersible solid particle system, particularly introduces rosmarinic acid as a natural high-efficiency antioxidant, forms an 'internal and external synergetic' antioxidant network with silybin, obviously delays the oxidation of diglyceride, and simultaneously enhances the stability of the system in intestinal environments. The rosmarinic acid can also form a composite layer with the fucoidan sulfate at the colloid interface, so that the physical stability and the slow release characteristic of the nano colloid are further improved.

Inventors

• ZHANG JINQIAO
• Zuo Xiaoting
• SUN SHUSEN
• ZHANG RUIPING

Assignees

• 畅唯生物科技(广州)有限公司

Dates

Publication Date

20260512

Application Date

20260302

Claims (8)

1. 1. The hypolipidemic diglyceride composition is characterized by being water-dispersible solid particles prepared by a hot-melt emulsification-cooling solidification process, and comprises the following components in percentage by weight: 30% to 60% of diglyceride, 30% To 55% of a structured matrix, 2% To 8% of phytosterol, 1 To 6% of an auxiliary functional composition, The balance being water; the structured matrix consists of amphiphilic starch and lecithin, wherein the amphiphilic starch is sodium starch octenyl succinate, and the weight ratio of the amphiphilic starch to the lecithin is 4:1 to 1.5:1; The auxiliary functional composition comprises silybin, fucoidan sulfate and rosmarinic acid, wherein the weight ratio of the silybin to the fucoidan sulfate to the rosmarinic acid is 1:5 (0.2-1) to 1:1 (0.5-2); in the diglyceride, the content of the 1, 3-diglyceride accounts for 80-100% of the total mass of the diglyceride.
2. 2. The hypolipidemic diglyceride composition according to claim 1, wherein the auxiliary functional composition further comprises galacto-oligosaccharides in an amount of 0.2% to 2% by weight of the total weight of the composition.
3. 3. The hypolipidemic diglyceride composition according to claim 1, wherein the structured matrix further comprises microcrystalline cellulose in an amount of 5% to 15% by weight of the structured matrix for enhancing the strength of the solidified network and regulating the rate of hydration of the particles.
4. 4. The hypolipidemic diglyceride composition according to claim 1, wherein the solid particles have a particle size of 0.2 mm to 1 mm.
5. 5. The hypolipidemic diglyceride composition according to claim 4, wherein the solid particles are dispersed in warm water at 40 to 60 ℃ with stirring to form a colloidal dispersion having an average particle diameter of less than 300 nm, and the colloidal dispersion is stable at room temperature for 2 hours.
6. 6. A method of preparing a hypolipidemic diglyceride composition according to any one of claims 1 to 5, comprising the steps of: s1, preparing an oil phase, namely mixing diglyceride, phytosterol, silybin and rosmarinic acid, heating to 70-80 ℃ and stirring to form a uniform and transparent molten oil phase; S2, preparing a water phase, namely dry-mixing sodium starch octenyl succinate, fucoidan and microcrystalline cellulose, dispersing in hot water at 60-70 ℃, stirring until the mixture is completely swelled to form a uniform glue solution, then adding lecithin and galactooligosaccharide, and uniformly mixing to obtain a homogeneous water phase matrix solution; S3, hot melt emulsification and structuring, namely adding the molten oil phase into the aqueous phase matrix liquid under the constant temperature condition of 65-75 ℃, firstly performing high-speed shearing and primary emulsification at the rotating speed of 5000-8000 rpm, then performing high-pressure homogenization on the mixture, and circularly homogenizing for 2-4 times under the pressure of 30-50 MPa to obtain hot melt emulsion; S4, program cooling, solidifying and granulating, namely transferring the hot melt emulsion into a program cooling device, firstly cooling to 30-40 ℃ at the rate of 8-15 ℃ per minute, preserving heat for 5-15 minutes, then cooling to below 10 ℃ at the rate of 3-8 ℃ per minute, solidifying the hot melt emulsion into a block, and finally crushing and screening the block to obtain the water-dispersible solid particles.
7. 7. The method according to claim 6, wherein intermittent low-frequency oscillation is applied to the material during the incubation period of the procedure cooling solidification and granulation steps, the frequency of the intermittent low-frequency oscillation being 20 hz to 50 hz, and the duration of the intermittent low-frequency oscillation being one third to one half of the total duration of incubation.
8. 8. Use of a hypolipidemic diglyceride composition according to any one of claims 1 to 5 for the preparation of a reconstituted solid beverage, nutritional bar or tablet for aiding in hypolipidemic.

Description

Blood fat reducing diglyceride composition and preparation method and application thereof Technical Field The invention relates to the technical field of biological medicines, in particular to a blood fat reducing diglyceride composition and a preparation method and application thereof. Background Hyperlipidemia is an independent risk factor for atherosclerotic cardiovascular disease, and its prevention has become a major topic in the world public health field. In addition to pharmaceutical treatments, dietary interventions based on specific functional ingredients are of great interest in the field of "medical foods" or "dietary supplements" because of their high safety and acceptance. Among them, diglycerides, especially 1, 3-diglycerides, are recognized as a functional fat with great potential because of their unique metabolic pathways, which are not easily re-esterified into triglycerides after hydrolytic absorption in the small intestine, and thus can effectively reduce postprandial blood lipids and fat accumulation in the body. However, the conversion of the superior laboratory conclusion of diglycerides to stable, efficient, user-friendly end products presents a systematic challenge in terms of formulation, which directly restricts its industrial application. The prior art scheme mainly has the following key defects: The current common proposal is to add diglyceride directly into beverage in liquid form or to make solid powder or capsule by simple physical mixing with dry powder such as plant sterol, etc. The former is used as a liquid preparation, oil-water delamination and oxidation rancidity are easy to occur, the shelf life is short, and the requirements on packaging and storage conditions are harsh, while the latter is solid, but is only physically mixed, the components lack synergistic protection, diglyceride microdrops can be aggregated and oxidized during storage, and the dispersibility of fat-soluble components in water is extremely poor, so that the bioavailability is seriously affected. For improved stability, spray-drying microencapsulation with gelatin/gum arabic and other wall materials is another technical route. However, the traditional process has the inherent defects that firstly, the embedding rate is limited, part of grease is exposed on the surface of particles and still is easy to oxidize, secondly, the wall material is mostly hydrophilic polymer, the core material is rapidly dissolved in water, so that 'burst release' of active ingredients in gastrointestinal tract cannot be realized, thirdly, the particle size of the obtained particles is large (generally more than 10 microns), the system is emulsion rather than colloid after being brewed, the physical stability is insufficient, and layering is still possible after standing. In the prior art, there is no lack of a scheme for compounding diglycerides with various plant extracts (such as tea polyphenol, curcumin) or chemical drugs. However, these approaches have focused on "addition" of pharmacological actions, ignoring the "compatibility" and "co-stabilization" designs of different physicochemical properties of the components (hydrophilic, lipophilic, amphiphilic) within the same delivery system. For example, if there is no suitable emulsifier and structured matrix for connection between the polysaccharide and the oil, the polysaccharide and the oil can be mixed in a solid state, and once the polysaccharide and the oil meet water, the polysaccharide and the oil can be rapidly separated, so that each component is released asynchronously, and the theoretical synergistic lipid-lowering effect cannot be exerted. The market needs a product which is convenient to carry and brew like common solid beverage and can be efficiently absorbed like nano emulsion. The prior art cannot solve the contradiction well that solid powder often sacrifices dispersity and absorption speed, and stable nanoemulsion is usually liquid, which is inconvenient for storage and transportation. The development of a solid-state delivery system capable of self-dispersing into nano colloid when meeting water is a clear but not well-realized technical target in the field of functional foods. Therefore, there is a need in the art for an innovative formulation strategy that must systematically solve the above-mentioned problems, firstly, by constructing a completely new solid matrix, stably solidifying liquid diglycerides, achieving excellent oxidative stability and physical stability, secondly, the solid spontaneously disperses into uniform and stable nanoscale colloids in the aqueous phase rapidly upon ingestion, greatly improving the biological accessibility of fat-soluble active ingredients, and finally, the system must intelligently support and cooperate with a variety of functional ingredients of different polarities (such as lipophilic phytosterols, silybin, hydrophilic fucoidan, and amphiphilic rosmarinic acid) so that they coexist stably upon storage and coope