CN-122005501-A - Soybean isolated protein-based composite particle loaded with bovine myocardial peptide and preparation method thereof

CN122005501ACN 122005501 ACN122005501 ACN 122005501ACN-122005501-A

Abstract

The invention relates to the technical field of biological embedding, and provides a soybean isolated protein-based composite particle loaded with bovine myocardial peptide and a preparation method thereof. The preparation method comprises dissolving soybean protein isolate, bovine myocardial peptide and sodium alginate in water, adjusting pH of the solution to drive self-assembly, magnetically stirring, centrifuging to obtain supernatant, and lyophilizing to obtain composite granule for encapsulating bovine myocardial peptide. The invention takes the soy protein isolate and the sodium alginate as wall materials, obviously relieves the degradation rate of the bovine myocardial peptide in vivo by optimizing the proportion of the embedding materials and the encapsulation conditions, effectively reserves the antioxidant capacity of the bovine myocardial peptide, improves the bioavailability of the bovine myocardial peptide, and can be used in a plurality of fields such as food, medicine and the like.

Inventors

ZHU JIE
GUO YANJIE
ZHANG HANDAN
LUO XIZI
Jia dongyang
SHI YUEXING

Assignees

西北农林科技大学

Dates

Publication Date: 20260512
Application Date: 20260203

Claims (4)

1. The soybean isolated protein-based composite particle loaded with the bovine myocardial peptide and the preparation method thereof are characterized by comprising the following steps: Dissolving a certain amount of Soy Protein Isolate (SPI) and 0.4 g Niu Xinji peptide (BMP, self-made by laboratory) in 20 mL deionized water, continuously stirring for 3.0 and h, and adjusting the pH of the solution to a preset range for later use; Dissolving a certain amount of Sodium Alginate (SA) in 20 mL deionized water until the solution is completely transparent; Slowly adding the soybean protein isolate-bovine myocardial peptide mixed solution into sodium alginate solution, regulating the pH of the mixed solution to a proper range, continuously stirring for 1.0 h, centrifuging at 8000 g for 10min to obtain supernatant, standing the supernatant at 4 ℃ for 24: 24h, and freeze-drying to obtain the soybean protein isolate-Niu Xinji peptide-sodium alginate composite particle powder.
2. The method for preparing the isolated soy protein-based composite particles loaded with the bovine myocardial peptide according to claim 1, wherein the pH values of the isolated soy protein-bovine myocardial peptide mixed solution are respectively 4.0, 5.0, 6.0, 7.0 and 8.0, the adding amounts of the isolated soy protein are respectively 0.0 g, 0.2 g, 0.4 g, 0.6 g and 0.8 g, the pH values of the isolated soy protein-sodium alginate mixed solution are respectively 3.0, 3.5, 4.0 and 4.5, and the stirring temperatures are respectively room temperature, 40 ℃, 50 ℃, 60 ℃ and 70 ℃. Subsequently, composite particles were prepared under established optimal conditions, wherein the total weight of soy protein isolate and sodium alginate was 0.3 g, and the mass ratio (SPI/SA) was 1:0, 4:1, 2:1, 1:1, 1:2, 1:4 and 0:1, respectively.
3. The preparation method of the soybean protein isolate-Niu Xinji peptide-sodium alginate composite granules according to the above-mentioned preparation method of claims 1 and 2.
4. The isolated soy protein-based composite particle loaded with bovine myocardial peptide and the preparation method thereof according to claim 3, wherein the encapsulation efficiency of the composite particle is 60.04-71.60%, the absolute value of Zeta potential is 16.40-43.50 mV, the DPPH free radical clearance of the composite particle after in vitro gastrointestinal digestion is 28.30-33.57%, and the absorbance value measured by iron ion reducing power is 0.13-0.17.

Description

Soybean isolated protein-based composite particle loaded with bovine myocardial peptide and preparation method thereof Technical Field The invention mainly relates to the technical field of biological embedding, in particular to composite particles for encapsulating bovine myocardial peptide, which are prepared by taking bovine myocardial peptide as a core active ingredient, taking soy protein isolate and sodium alginate as main wall materials, and driving the soy protein isolate and the sodium alginate to self-assemble by adjusting the pH value of a solution. Background The bioactive peptide is composed of different amino acids in different arrangement modes, and has multiple functions of resisting oxidation, reducing blood sugar, regulating immunity and the like. In recent years, bioactive peptides are increasingly being used in the fields of health foods and medicines. However, bioactive peptides are very susceptible to degradation in processing, transport, and the gastrointestinal environment, which also severely limits their use. Therefore, the wall material is selected as a wall material for loading and delivering the bioactive peptide, so that not only can the bad smell of the bioactive peptide be covered, but also the stability of the bioactive peptide when the bioactive peptide passes through the gastrointestinal tract can be improved, and the application range of the bioactive peptide in the fields of foods and medicines can be widened. The isolated soy protein is one of the most common vegetable proteins and is widely applied in the food industry due to the advantages of high nutritional value, good biocompatibility, low cost and the like. The peptide chain of the isolated soy protein molecule is rich in hydrophobic amino acid residues, which can promote hydrophobic interaction between the isolated soy protein and the hydrophobic bioactive molecule, and the presence of the charged amino acid residues can enable the isolated soy protein to combine with the bioactive molecule to form a complex through electrostatic interaction. Based on the above characteristics, soy protein isolates have been widely used as carriers for delivering bioactive substances in recent years. Sodium alginate is used as a natural linear anionic polysaccharide, has the advantages of low cost, high biocompatibility, relatively stable environment and the like, is classified as a safe food additive by the United states Food and Drug Administration (FDA) and is widely used as a functional food raw material for developing functional foods, and meanwhile, the linear structure of the sodium alginate can form an ordered network structure with protein, so that the stability of the protein is improved. Therefore, the invention takes the bovine myocardial peptide as a core active ingredient, takes the soybean protein isolate and the sodium alginate as wall materials, and prepares the composite particles by self-assembly through electrostatic interaction, hydrophobic interaction and the like between the protein and the polysaccharide so as to improve the bioavailability of the bovine myocardial peptide. Disclosure of Invention Aiming at the key technical problems faced in the technical field of bioactive factor encapsulation and delivery, the invention provides a soybean isolated protein-based composite particle loaded with bovine myocardial peptide and a preparation method thereof, and aims to research a way of delivering bioactive peptide by protein-polysaccharide composite embedding, and simultaneously expand the application range of bioactive peptide in the fields of foods, medicines, cosmetics and the like. In order to solve the technical problems, the invention provides the following technical scheme: Dissolving 0.4 g soybean protein isolate and 0.4 g bovine myocardial peptide in 20mL deionized water, continuously stirring at room temperature for 3.0 h, adjusting the pH of the solution to 4.0 for standby, dissolving 0.2 g sodium alginate in 20mL deionized water until the solution is completely transparent, slowly adding the soybean protein isolate-bovine myocardial peptide mixed solution into the sodium alginate solution, adjusting the pH of the mixed solution to 3.5, continuously stirring at room temperature for 1.0 h, centrifuging at 8000 g for 10min to obtain supernatant, standing the supernatant at 4 ℃ for 24 h, and freeze-drying to obtain the soybean protein isolate-Niu Xinji peptide-sodium alginate composite particle powder. The composite particles obtained are subjected to related index measurement, the Zeta potential value of the composite particles is-37.0 mV, the DPPH free radical clearance rate of the composite particles after in-vitro gastrointestinal digestion is 31.52, the iron ion reducibility absorbance value is 0.17, and compared with a control group which only uses soy protein isolate as a wall material for packaging, the degradation rate of bovine myocardial peptide in the body is obviously relieved by using th