Search

CN-122005504-A - PH responsive lactobacillus plantarum gel ball and preparation method and application thereof

CN122005504ACN 122005504 ACN122005504 ACN 122005504ACN-122005504-A

Abstract

The invention discloses a pH response type lactobacillus plantarum gel ball and a preparation method and application thereof, and belongs to the technical field of embedding and delivering of probiotics. The pH response type lactobacillus plantarum gel ball provided by the invention has a compact gel network structure, can obviously improve the survival rate of probiotics in gastrointestinal fluid, has the effect of targeted release in intestinal tracts, has a synergistic effect with the probiotics, has an effect obviously superior to that of free probiotics and empty gel in relieving constipation, is simple and easy to operate, has high safety of used reagents and materials, does not use toxic and harmful reagents in the preparation process, does not generate toxic and harmful substances, is environment-friendly, and has no potential safety hazard.

Inventors

  • YOU LIJUN
  • DUAN TING
  • MA CONG
  • ZONG ZIHAO

Assignees

  • 华南理工大学

Dates

Publication Date
20260512
Application Date
20260310

Claims (10)

  1. 1. A pH response type lactobacillus plantarum gel ball and a preparation method and application thereof are characterized in that the gel ball takes lactobacillus plantarum as a core material, takes pectin, laminarin and whey protein as composite wall materials, and is formed by double ion crosslinking and curing of internal calcium carbonate and external calcium chloride solution.
  2. 2. The method for preparing the pH-responsive lactobacillus plantarum gel ball according to claim 1, which comprises the following steps: (1) Activating lactobacillus plantarum, and then adjusting the concentration of bacteria by using sterile distilled water to obtain free lactobacillus plantarum mother liquor; (2) Preparing a wall material solution, namely respectively dissolving pectin and laminarin in sterile distilled water, uniformly mixing according to a certain volume ratio, adding whey protein powder, uniformly mixing, adding calcium carbonate powder, uniformly stirring, and eliminating bubbles to obtain the wall material solution; (3) Embedding lactobacillus plantarum, uniformly mixing the free lactobacillus plantarum mother solution prepared in the step (1) with the wall material solution prepared in the step (2), and then dripping the mixture into the curing liquid through a microfluidic injection pump for crosslinking and curing to obtain the pH response type lactobacillus plantarum gel ball.
  3. 3. The method for preparing the pH responsive lactobacillus plantarum gel ball according to claim 2, wherein the lactobacillus plantarum in the step (1) is activated and cultured by adopting MRS broth culture medium at 37 ℃, is centrifuged for 10-15min at 4 ℃ and 8000 Xg before the concentration of the lactobacillus plantarum is adjusted by using sterile distilled water, and is washed for 2-3 times by using the sterile distilled water.
  4. 4. The method for preparing the pH-responsive lactobacillus plantarum gel pellet as claimed in claim 3, wherein the MRS broth culture medium is composed of 10.0g of casein enzyme digest, 10.0g of beef extract powder, 4.0g of yeast extract powder, 2.0g of tri-ammonium citrate, 5.0g of sodium acetate, 0.2g of magnesium sulfate (MgSO 4 ·7H 2 O), 0.05g of manganese sulfate (MnSO 4 ·4H 2 O), 2.0g of dipotassium hydrogen phosphate, 20.0g of glucose and 1.08g of Tween-80 (final pH 5.7+ -0.2) per liter.
  5. 5. The method of claim 2, wherein the concentration of the free Lactobacillus plantarum is adjusted to 1-3X 10 10 CFU/mL.
  6. 6. The method for preparing the pH responsive lactobacillus plantarum gel ball according to claim 2, wherein galacturonic acid (dry basis) in pectin in the step (2) is more than or equal to 74.0%, the molecular weight of laminarin is 10-260kDa, and the total sugar content is 62-78%.
  7. 7. The method for preparing the pH responsive lactobacillus plantarum gel ball according to claim 2, wherein the mass-volume ratio of pectin to laminarin to whey protein powder to calcium carbonate powder to sterile distilled water in the step (2) is 1g to 0.1g to 100mL-3g to 0.2g to 100mL, and a method of standing at room temperature is adopted for eliminating bubbles.
  8. 8. The method for preparing the pH responsive lactobacillus plantarum gel ball according to claim 2, wherein the volume ratio of the free lactobacillus plantarum mother solution to the wall material solution in the step (3) is 1:5.
  9. 9. The preparation method of the pH response type lactobacillus plantarum gel ball according to claim 2, wherein the method for crosslinking and solidifying the micro-fluidic syringe pump in the step (3) is characterized in that the propelling speed of the micro-fluidic syringe pump is 0.5-5mL/min, the caliber of a needle is 0.2-0.8mm, the distance between the needle and the liquid level of the solidifying liquid is 10-20cm, and the crosslinking and solidifying are carried out at room temperature.
  10. 10. Use of the pH-responsive lactobacillus plantarum gel pellet of claim 1 for the treatment and/or alleviation of constipation.

Description

PH responsive lactobacillus plantarum gel ball and preparation method and application thereof Technical Field The invention relates to the technical field of embedding and delivering probiotics, in particular to a pH response type lactobacillus plantarum gel ball and a preparation method and application thereof. Background With the improvement of health consciousness of people, probiotics are attracting more and more attention because of the beneficial effects of regulating intestinal flora, enhancing immunity, improving intestinal health and the like. Lactobacillus plantarum is used as a common probiotic and has various health effects of regulating intestinal flora, enhancing immunity, relieving constipation and the like. It is thought that only the number of live bacteria ingested exceeding 10 6 CFU/g will have a beneficial effect on the host. However, oral free probiotics undergo attack by strong acids of the stomach (pH 1.5-2.0) and digestive enzymes before reaching the intestine, possibly resulting in a significant inactivation of the probiotic and failure to fully exert the probiotic effect. At present, an embedding technology is often adopted to protect probiotics. Common probiotic embedding techniques are spray drying, freeze drying, emulsifying, extrusion, electrostatic spraying, and the like. Among them, the hydrogel prepared by extrusion method is widely used for tissue repair and drug delivery due to simple operation and good nutrient retention. However, the single protein/polysaccharide gel has the problems of low embedding rate, easy decomposition, early release of probiotics and the like, and has limited protective effect on the probiotics, and the composite gel system can effectively improve the defects. Sodium alginate-calcium ion crosslinking systems are one of the most common methods (as in patent CN114672480 a), but they have limited protective ability in acidic environments and poor controllability of release behavior. In order to further improve the protection effect, the prior art mostly adopts multi-layer embedding (such as adopting a three-layer structure of sodium alginate-chitosan-hydroxypropyl methyl cellulose in patent CN 119366639A), but the method has complex process and multiple steps, and needs to use an organic solvent (such as acetic acid for dissolving chitosan), which is not beneficial to large-scale production and food application. Therefore, developing a probiotic delivery system that has a simple process, is safe in materials, can resist gastrointestinal digestion, and can be accurately released in the intestinal tract is a technical problem to be solved in the art. Disclosure of Invention The invention aims to provide a preparation method of a pH response type lactobacillus plantarum gel ball, which aims to solve the problems of low embedding rate, poor stability, complex process, complicated steps and hidden danger of food safety existing in the existing embedding technology. In order to achieve the above purpose, the invention provides a pH responsive lactobacillus plantarum gel ball, a preparation method and application thereof, wherein the gel ball is prepared by performing double-ion cross-linking curing on internal calcium carbonate and external calcium chloride solution by taking lactobacillus plantarum as a core material and pectin, laminarin and whey protein as composite wall materials. The preparation method and application of the pH response type lactobacillus plantarum gel ball comprise the following steps: (1) Activating lactobacillus plantarum, and then adjusting the concentration of bacteria by using sterile distilled water to obtain free lactobacillus plantarum mother liquor; (2) Preparing a wall material solution, namely respectively dissolving pectin and laminarin in sterile distilled water, uniformly mixing according to a volume ratio, adding whey protein powder, uniformly mixing, adding calcium carbonate powder, uniformly stirring, and eliminating bubbles to obtain the wall material solution; (3) Embedding lactobacillus plantarum, uniformly mixing the free lactobacillus plantarum mother solution prepared in the step (1) with the wall material solution prepared in the step (2), and then dripping the mixture into the curing liquid through a microfluidic injection pump for crosslinking and curing to obtain the pH response lactobacillus plantarum gel balls. Preferably, the lactobacillus plantarum in the step (1) is activated and cultured by adopting MRS broth culture medium at 37 ℃, and is centrifuged for 10-15min at the temperature of 4 ℃ and 8000 Xg before the concentration of the lactobacillus plantarum is adjusted by using sterile distilled water, and is washed for 2-3 times by using the sterile distilled water. Preferably, the MRS broth medium is composed of 10.0g of casein digest, 10.0g of beef extract powder, 4.0g of yeast extract powder, 2.0g of tri-ammonium citrate, 5.0g of sodium acetate, 0.2g of magnesium sulfate (MgSO 4·7H2 O), 0.05g of ma