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CN-122005523-A - Biological application of sodium caproate

CN122005523ACN 122005523 ACN122005523 ACN 122005523ACN-122005523-A

Abstract

The invention discloses biomedical application of sodium caproate. The invention provides and verifies for the first time that sodium caproate causes the epilepsy of the zebra fish in the low concentration range of 10-100 mu M and inhibits the epilepsy in the high concentration range of 10-100 mM, namely, the sodium caproate has a bidirectional dose effect in the zebra fish nervous system, and based on the two-way dose effect, the method can be used for the construction of a zebra fish epilepsy model and the research of an antiepileptic mechanism.

Inventors

  • ZHANG QI
  • ZHANG XIN
  • SHEN DINGDING
  • WANG QIAN

Assignees

  • 南通大学

Dates

Publication Date
20260512
Application Date
20260327

Claims (10)

  1. 1. Use of sodium caproate for modulation of the nervous system of an animal for non-diagnostic or therapeutic purposes.
  2. 2. The use according to claim 1, wherein the sodium caproate has CAS number 10051-44-2.
  3. 3. The use according to claim 1, wherein the animal is a vertebrate.
  4. 4. The use according to claim 3, wherein the vertebrate is zebra fish.
  5. 5. The use according to claim 4, wherein the use is the use of sodium caproate in the construction of a zebra fish epilepsy model.
  6. 6. The method according to claim 5, wherein the step of culturing the zebra fish embryo to hatching, and culturing the zebra fish embryo with a culture medium containing sodium caproate to complete the zebra fish epilepsy model construction.
  7. 7. The use according to claim 5, wherein the sodium caproate is exposed at a concentration of 10 to 100 μm.
  8. 8. The use according to claim 4, wherein the use is of sodium caproate in anti-epileptic mechanism studies.
  9. 9. The use according to claim 8, wherein the epilepsy is epilepsy caused by a decrease in inhibitory neurotransmission.
  10. 10. The use according to claim 8, wherein the exposure concentration of sodium caproate in the use is 10-100 mM.

Description

Biological application of sodium caproate Technical Field The invention relates to the field of neurobiology, in particular to biological application of sodium caproate. Background Epilepsy is one of the most common neurological disorders worldwide, the nature of which is a brain disorder characterized by recurrent and spontaneous attacks. The onset of the drug is derived from the excessive synchronous discharge of brain cells, which leads to various clinical manifestations such as convulsion, consciousness loss and the like, and has the characteristics of repeatability, transiently and notch board property. In the event of onset, patients often have altered respiratory, cardiac rhythm and autonomic nervous function, resulting in increased risk of hypoxia and abnormal heart rate, and often have mental disorders such as anxiety and depression. At present, the pathogenesis of the nerve cell is not completely elucidated, and various causes such as heredity, structure, metabolism, immunity and the like are known to be involved, so that ion channel dysfunction, neurotransmitter imbalance, nerve network excitability increase and the like are caused. Genetic studies have found that epilepsy is highly heterogeneous, has rare single-gene mutations and complex polygenic genetic patterns, and has a common genetic basis with mental disorders. In view of the complexity of human epilepsy, it is important to build a reliable animal model. Zebra fish (Danio rerio) is used as a common model organism, and has unique advantages in epileptic research, namely, firstly, the zebra fish has rapid nervous system development, complete central nervous structure and functional blood brain barrier after 5 days of fertilization, secondly, the zebra fish has high homology with human genes, about 84% of human disease genes can find homologous genes in the zebra fish, and the zebra fish has strong reproductive capacity and low breeding cost, thus being very suitable for high-throughput drug screening. At present, the construction of a zebra fish epilepsy model is mainly realized by inducing acute epileptiform attacks of the zebra fish through chemical inducers such as gamma-aminobutyric acid receptor antagonists of pentylton, rhodophycin and the like, however, the compounds mainly induce excitatory neurotoxicity and lack a model for realizing bidirectional regulation. Disclosure of Invention The invention aims to provide the application of sodium caproate in regulation of animal nervous system non-diagnosis or treatment purposes, including the application of low-concentration sodium caproate in constructing a zebra fish epilepsy model and the application of high-concentration sodium caproate in anti-epilepsy mechanism research. The technical scheme is that the sodium caproate is applied to the regulation of non-diagnosis or treatment purposes of an animal nervous system. Preferably, the CAS number of the sodium caproate is 10051-44-2. Preferably, the animal is a vertebrate, and more preferably, the vertebrate is a zebra fish. Preferably, the application is the application of sodium caproate in constructing a zebra fish epilepsy model, and further preferably, the application comprises the steps of culturing zebra fish embryos until hatching, and feeding the zebra fish embryos by using a culture medium containing sodium caproate to finish constructing the zebra fish epilepsy model, wherein the exposure concentration of the sodium caproate is 10-100 mu M. Preferably, the application is the application of sodium caproate in anti-epileptic mechanism research, and further preferably, the epilepsia is epilepsia caused by inhibitory neurotransmission reduction, and the exposure concentration of sodium caproate in the application is 10-100 mM. Compared with the prior art, the invention has the advantages that the invention firstly provides and verifies that sodium caproate causes the epilepsy of the zebra fish in the low concentration range of 10-100 mu M and inhibits the epilepsy in the high concentration range of 10-100 mM, namely, the sodium caproate has bidirectional dose effect in the zebra fish nervous system, and based on the two-way dose effect, the invention can be used for the construction of a zebra fish epilepsy model and the research of an antiepileptic mechanism. Drawings FIG. 1 is a cluster heat map of differential metabolites enriched from metabolome analysis of wild type GABRG and I107T mutant GABRG2 zebra fish; FIG. 2 is a statistical graph of differences in caproic acid metabolism in wild-type GABRG and I107T mutant GABRG2 zebra fish; FIG. 3 is a graph of motion trajectories of zebra fish after treatment with sodium caproate at different concentrations; FIG. 4 is a statistical graph of swimming distance of zebra fish after low concentration sodium caproate treatment; FIG. 5 is a graph showing the time-to-exercise ratio of the total observed time period of zebra fish after low concentration sodium caproate treatment; FIG. 6 is a s