CN-122005562-A - Application of arecoline derivative in killing Bendengzhen in marine culture fish

Abstract

The invention belongs to the technical field of aquaculture disease control, and discloses application of arecoline derivatives in killing budworms. Through a coarse screening experiment and a soaking toxicity test and a dose effect experiment, the method has the advantages that in the coarse screening, the killing rate of 0.5mg/L arecoline derivative to the budworm reaches 75% after being soaked for 90min, the method is obviously superior to other five arecoline derivatives such as triclabendazole, arecoline and the like, the survival rate of golden pomfret after the arecoline derivative is soaked for 90min is 100% without obvious toxicity, and the dose effect experiment further proves that the killing rate of 0.7mg/L arecoline derivative to the budworm is improved to 95.35%. The arecoline derivative has the advantages of remarkable effect of killing the buddhist, high safety to host fishes and no obvious residual risk, can be further developed into special prevention and control of the buddhist in aquaculture, provides an effective scheme for solving the breeding diseases caused by the buddhist, and has a wide application prospect.

Inventors

• LIU TIANQIANG
• YU QING
• WANG ERLONG
• LIU MINGZHU
• XUE YUHE
• Gao Longkun

Assignees

• 西北农林科技大学
• 广西科学院
• 西北农林科技大学深圳研究院

Dates

Publication Date

20260512

Application Date

20260202

Claims (5)

1. 1. The application of the arecoline derivative in killing the bundan is characterized in that the arecoline derivative is treated in a soaking way for 90min at the concentration of 0.5mg/L, the killing rate of the arecoline derivative on the bundan is not lower than 75%, and the killing rate of the arecoline derivative on the bundan is not lower than 95% at the same soaking time when the concentration of the arecoline derivative is 0.7 mg/L.
2. 2. The use according to claim 1, wherein the arecoline derivative is used by soaking in a culture fish infected with buddhist for a period of 90min.
3. 3. The use according to claim 1, wherein the betulin derivative has a safe concentration of 0.7mg/L for golden pomfret, the survival rate of golden pomfret after soaking for 90min is 100%, and the survival rate of golden pomfret is significantly reduced when the concentration is higher than 1 mg/L.
4. 4. The use according to claim 1, wherein the application object of the present invention for controlling buddhist comprises golden pomfret, which can be extended to other economic farmed fish parasitized by buddhist.
5. 5. The betulin derivative is used for killing Benzoneworm and is characterized in that the effective use concentration range is 0.5mg/L-0.7mg/L, and the betulin derivative is treated for 90min by adopting a soaking method when in use.

Description

Application of arecoline derivative in killing Bendengzhen in marine culture fish Technical Field The invention belongs to the technical field of aquaculture, relates to prevention and control of parasitic diseases of aquatic animals, and in particular relates to application of arecoline derivatives in killing Benzonewort insects, and is particularly suitable for prevention and control of the parasitic diseases of Benzonewort insects of economic fishes such as golden pomfret and the like. Background The Nedendra (Benedenia spp.) is a common ectoparasite for aquaculture, belongs to the class of monoschistosome (Monogenea), and mainly parasitizes body surfaces, gills and fin parts of important economic fishes such as golden pomfret, groupers and the like. The parasite sucks body fluid and epidermal tissue of fish through the mouth organ, which causes damage to the skin of the host, bleeding and inflammation, and causes secondary bacterial or fungal infection, meanwhile, the parasite can cause the enhancement of stress response, the reduction of ingestion ability and slow growth of fish, and the parasite can cause large-scale death of the cultured population when serious, thereby bringing great economic loss to the aquaculture industry. At present, prevention and treatment of the budworm in aquaculture mainly depend on chemical drug soaking, and common drugs comprise furazolidone, formalin dilute water bath, mebendazole solution and the like. However, the existing general defects are that the killing effect is poor and even the killing activity is not achieved on the Nedendrania, the toxicity on the host fish is higher although the killing effect is achieved on part, the damage to the fish body is easy to cause, the Nedendrania can generate drug resistance after long-term use, and the control effect is further reduced. Therefore, searching for the safe control of the Bentonite insects with high efficiency and low toxicity becomes a key requirement for solving the current trouble of preventing and controlling the Bentonite insects. Natural products or specific chemical monomers become an important direction for preventing and developing aquatic parasites because of the characteristics of unique action mechanism and high safety to hosts. Through screening experiments on six arecoline derivatives, the research team discovers that the arecoline derivative has remarkable killing activity on the budworm, has low toxicity on host fishes, has the potential of developing special control, and provides a new scheme for solving the disease problem of the budworm. Disclosure of Invention In order to overcome the defects of poor control and killing effects and high host toxicity of the existing Bentonite, the invention provides the application of 1,3 (2H) -Pyridinedicarboxylic acid, 5,6-dihydro-4-hydroxy-,1- (1, 1-DIMETHYLETHYL) 3-ETHYL ESTER (hereinafter simply referred to as D) in killing Bentonite, defines the killing effect, safe concentration and using mode thereof, and provides a high-efficiency and safe technical means for controlling the Bentonite diseases in aquaculture. Further, in the application, the killing effect of D on the bundan insects has concentration dependency, namely, the killing rate of the D on the bundan insects after soaking for 90min reaches 75% when the concentration is 0.5mg/L, the killing rate of the D on the bundan insects after soaking for 90min is 88.37% when the concentration is 0.6mg/L, and the killing rate of the D on the bundan insects after soaking is 95.35% when the concentration is increased to 0.7mg/L, so that the bundan insects parasitic on the fish bodies can be effectively removed. Further, in the above application, the safe concentration of D to the host fish (exemplified by golden pomfret) is 0.7mg/L, the survival rate of golden pomfret is 100% after soaking for 90min, no abnormal behavior or body surface damage is caused, and when the concentration exceeds 1mg/L, the survival rate of golden pomfret is significantly reduced (the survival rate is only 33.3% when 1 mg/L), so that 0.7mg/L is determined as the optimal use concentration. Further, in the application, the use mode of the D is soaking treatment, namely the D is dissolved in a culture water body to prepare a solution of 0.5mg/L-0.7mg/L, and the fish infected with the Neden worm is placed into the solution to be soaked for 90min, so that the Neden worm can be killed efficiently, and the operation is simple and easy to popularize. The invention also provides a betulin derivative for killing the buddhist, which takes D as the only active ingredient, has the effective use concentration of 0.7mg/L, is treated for 90 minutes by adopting a soaking method when in use, and can simultaneously meet the dual requirements of killing the buddhist efficiently and guaranteeing the safety of host fishes. Compared with the prior art, the betulin derivative has the beneficial effects that 1. The betuline derivative has remarkable killing