CN-122005605-A - Application of Sargassum horneri polysaccharide in preparation of product for preventing or repairing skin photoaging and endogenous DNA injury caused by ultraviolet rays

Abstract

The invention discloses a new application of Sargassum horneri polysaccharide in preparing a product for preventing or repairing skin photoaging caused by ultraviolet rays and endogenous DNA damage. Experimental researches show that the Sargassum horneri polysaccharide provided by the invention can reduce UV-induced oxidative stress and ROS accumulation, reduce apoptosis and cycle retardation, and relieve UV-induced DNA double-strand break damage, thereby realizing the dual effects of ultraviolet radiation protection and DNA damage repair, solving the defect that the existing sun-proof product can only passively protect, and also making up the defect that the existing repair product cannot repair DNA damage. The Sargassum horneri polysaccharide has definite physical and chemical characteristics, stable activity, no irritation of chemical synthesis components, excellent biocompatibility and suitability for long-term use, is derived from natural Sargassum horneri, is suitable for cosmetics and medicines, can provide safe and effective natural raw materials for daily skin photoprotection, can provide new treatment ideas and product selection for clinical skin photodamage prevention and treatment, and has wide market application prospect.

Inventors

• ZHANG YIPING
• FANG HUA
• XI XIAOMIN
• LUO CHUNPING
• ZHENG HAILONG
• QIAN JIAHUI
• CHEN RUTING
• YANG GUANGLE
• CHEN WEIZHU
• JIN WENHUI

Assignees

• 自然资源部第三海洋研究所

Dates

Publication Date

20260512

Application Date

20260302

Claims (10)

1. 1. The application of Sargassum horneri polysaccharide in preparing product for preventing or repairing skin photoaging and endogenous DNA injury caused by ultraviolet is provided.
2. 2. The use according to claim 1, wherein the sargassum horneri polysaccharide consists of two main polysaccharides, one having a molecular weight distribution of 8-12 kda and the other having a molecular weight distribution of 3-6 kda.
3. 3. The use according to claim 1, characterized in that the content of aldonic acid in the sargassum horneri polysaccharide is 15-20%, the content of sulfate radical is not less than 9%.
4. 4. Use according to claim 1, characterized in that the ultraviolet radiation is UVA and/or UVB radiation.
5. 5. The use according to claim 1, wherein said sargassum horneri polysaccharide is capable of reducing UV-induced oxidative stress and ROS accumulation, reducing apoptosis and cycle arrest, and reducing UV-induced DNA double strand break damage, thereby achieving dual efficacy of UV radiation protection and DNA damage repair.
6. 6. The use according to claim 1, characterized in that the preparation method of sargassum horneri polysaccharide comprises the following steps: (1) Adding 1-3% acetic acid solution into Sargassum horneri powder, heating for extraction, filtering, collecting supernatant, centrifuging, filtering, and concentrating to obtain concentrated solution; (2) Adding absolute ethyl alcohol into the concentrated solution to enable the concentration of the ethanol to reach 30%, centrifuging, continuously adding absolute ethyl alcohol into the supernatant to enable the concentration of the ethanol to reach 80%, standing at 4 ℃ overnight, centrifuging, collecting precipitate, and drying to obtain the Sargassum horneri polysaccharide.
7. 7. The use according to claim 6, wherein in the step (1), the feed-liquid ratio is 1:20-30 g/mL, and the extraction time is 2-3 h.
8. 8. The use according to claim 1, wherein the product is a pharmaceutical or cosmetic product.
9. 9. The use of claim 8, wherein the cosmetic further comprises one or more of a moisturizer, an emollient, a lipid, an emulsifier, a preservative, a plant extract, or a physical sunscreen, and wherein the pharmaceutical product further comprises a pharmaceutically acceptable carrier.
10. 10. The use according to claim 8, wherein the cosmetic is an aqueous solution, a concentrate, an emulsion, a cream, a gel, a mask or a spray.

Description

Application of Sargassum horneri polysaccharide in preparation of product for preventing or repairing skin photoaging and endogenous DNA injury caused by ultraviolet rays Technical Field The invention belongs to the technical field of biological medicines and cosmetics, and particularly relates to application of Sargassum horneri polysaccharide in preparation of a product for preventing or repairing skin photoaging and endogenous DNA damage caused by ultraviolet rays. Background Ultraviolet (UV) radiation is the major environmental factor responsible for skin damage, where UVA (320-400 nm) penetrates into the dermis of the skin, causing long-term photoaging, and UVB (280-320 nm) energy is higher and is strongly absorbed by the epidermis layer of the skin, which is the major factor responsible for skin photodamage. Long-term or excessive exposure to UVB may cause acute sunburn, erythema, chronic inflammation, oxidative damage, collagen degradation, elastosis, and ultimately skin wrinkles, melanin pigmentation, and even skin cancer in severe cases. The damage mechanism is mainly characterized in that on one hand, UVB irradiation can excite skin cell endogenous photosensitizers to cause a large amount of intracellular Reactive Oxygen Species (ROS) to be generated and accumulated, the dynamic balance of intracellular oxidation and an antioxidant system is destroyed to trigger oxidative stress reaction, on the other hand, photon energy of UVB can be directly absorbed by DNA bases in cells to induce the formation of Cyclobutane Pyrimidine Dimers (CPDs) and pyrimidine (6-4) photoproducts (6-4 PPs) between the bases and the pyrimidine, and the accumulation of CPDs and 6-4 PPs can block DNA replication and transcription to interfere with normal physiological functions of cells, and if continuous DNA damage cannot be effectively repaired, the increase of gene mutation and canceration risks can be caused to force the cells to trend to aging, apoptosis or necrosis. At present, in the anti-photoaging field, active ingredients such as vitamin C, polyphenols and the like can effectively remove free radicals and relieve oxidative damage, but the action mechanism of the active ingredients is still limited. The components mainly focus on antioxidation defense, lack active repair capability on DNA physical damage (such as pyrimidine dimer and double strand break) directly caused by ultraviolet rays, and limit practical application effects due to chemical stability and transdermal absorption efficiency. In recent years, polysaccharides have received increasing attention due to their excellent biocompatibility, low toxicity and multi-target regulatory properties. Sargassum horneri is the whole body of Sargassum horneri Sargassum horneri (Turn) c.ag. Salty and cold taste, has the effects of softening hardness to dissipate stagnation, relieving swelling and heat, promoting diuresis and reducing phlegm, contains algin, fucoidin and the like, and is mainly distributed in coastal areas of China. As marine drug resource, sargassum horneri has the characteristics of high growth speed, strong regeneration capacity and the like, and is a sustainable ecological resource. The prior art only discloses that the Sargassum horneri polysaccharide has the conventional effects of moisturizing, resisting oxidation and the like, and has no report on the application of Sargassum horneri polysaccharide in preparing products for preventing or repairing skin photoaging and endogenous DNA damage caused by ultraviolet rays. Disclosure of Invention In view of the above, the invention aims to provide an application of Sargassum horneri polysaccharide in preparing a product for preventing or repairing skin photoaging and endogenous DNA damage caused by ultraviolet rays, wherein the Sargassum horneri polysaccharide has definite physical and chemical characteristics and stable activity, and can realize double effects of ultraviolet radiation protection and DNA damage repair. In order to achieve the above object, the technical scheme of the present invention is realized by the following method: the invention provides an application of Sargassum horneri polysaccharide in preparing a product for preventing or repairing skin photoaging and endogenous DNA damage caused by ultraviolet rays. As an optimal implementation mode, the Sargassum horneri polysaccharide is derived from Sargassum horneri Sargassum horneri, is obtained through acid extraction and alcohol precipitation, and consists of two main polysaccharides, wherein the molecular weight of one polysaccharide is 8-12 kDa, and the molecular weight of the other polysaccharide is 3-6 kDa. As a preferable implementation mode, the content of the aldonic acid in the Sargassum horneri polysaccharide is 15-20%, the content of sulfate radical is more than or equal to 9%, and the Sargassum horneri polysaccharide with the specific structure not only has ultraviolet radiation resistance and antioxidant activity, but al