Search

CN-122005609-A - Heterojunction nano-enzyme and preparation method and application thereof

CN122005609ACN 122005609 ACN122005609 ACN 122005609ACN-122005609-A

Abstract

The invention discloses a heterojunction nano-enzyme and a preparation method and application thereof, wherein cubic Co 3 O 4 (PCC NPs) is synthesized firstly by a hydrothermal method, the molar ratio of the cubic Co 3 O 4 to a zinc source and a cadmium source is changed, and a series of heterojunction nano-enzymes are synthesized under the conditions of high temperature and high pressure. The invention designs and prepares the novel PCC@ZnCdS heterojunction nano enzyme, successfully improves the proportion of Co 2+ /Co 3+ in the material, and remarkably enhances the ROS scavenging capability. The PCC@ZnCdS heterojunction shows good multienzyme activities such as superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx) and Catalase (CAT), so that the maximization of antioxidant activity is realized, the material can release endogenous H 2 S, inhibit inflammatory factor release and inhibit NLRP3 inflammatory body activation, and a new strategy is provided for the treatment of colonitis.

Inventors

  • ZHAO WENBO
  • WU YAXIN

Assignees

  • 南京师范大学

Dates

Publication Date
20260512
Application Date
20260215

Claims (10)

  1. 1. A heterojunction nano-enzyme is characterized in that cubic Co 3 O 4 (PCC NPs) is synthesized firstly by a hydrothermal method, the molar ratio of the cubic Co 3 O 4 to a zinc source and a cadmium source is changed, and a series of heterojunction nano-enzymes are synthesized under the conditions of high temperature and high pressure.
  2. 2. The heterojunction nanoenzyme of claim 1, wherein the heterojunction nanoenzyme is in a cubic structure and has nanospheres uniformly grown around the cube in a size of about 230-270 nm a.
  3. 3. A method for preparing the heterojunction nano-enzyme as defined in claim 1, comprising the following steps: (1) Dissolving Co (NO 3 ) 2 ·6H 2 O and NaOH respectively in deionized water, dropwise adding a NaOH solution into the Co (NO 3 ) 2 solution under stirring, continuously stirring, performing hydrothermal reaction on the mixed solution to obtain a precursor, and calcining the dried precursor to obtain a target product PCC NPs; (2) Dissolving Zn (Ac) 2 ·2H 2 O and Cd (Ac) 2 ·2H 2 O in deionized water, adding Thioacetamide (TAA) after ultrasonic treatment, continuously stirring, dissolving NaOH in the deionized water, pouring the solution into the deionized water, continuously stirring, and after stirring, washing and drying a sample obtained by high-temperature reaction of the mixture to finally obtain ZnCdS NPs; (3) Dissolving PCC NPs in deionized water, and performing ultrasonic treatment to obtain a product A, dissolving Zn (Ac) 2 ·2H 2 O and Cd (Ac) 2 ·2H 2 O in deionized water, performing ultrasonic treatment to obtain a product B, adding the solution B into the solution A, adding TAA under stirring, and performing ultrasonic stirring to obtain a product C; (4) And adding NaOH solution into the product C, fully stirring, and reacting at high temperature to obtain the product PCC@ZnCdS NPs.
  4. 4. The method for preparing heterojunction nano-enzyme according to claim 1, wherein the feeding molar ratio of NaOH to Co (NO 3 ) 2 ·6H 2 O) in the step (1) is 1-6:1-1.
  5. 5. The preparation method of the heterojunction nano-enzyme according to claim 1 is characterized in that the feeding molar ratio of Zn (Ac) 2 ·2H 2 O to Cd (Ac) 2 ·2H 2 O in the step (2) is 1-2:1-1, and the feeding molar ratio of Cd (Ac) 2 ·2H 2 O to TAA in the step (2) is 1-5:1-2.
  6. 6. The method for preparing heterojunction nano-enzyme according to claim 1, wherein in the step (3), the charging mole ratio of PCC NPs to TAA is 1-5:1-2.
  7. 7. The method for preparing heterojunction nano-enzyme according to claim 1, wherein the temperature of the hydrothermal reaction in the step (4) is 120-180 ℃ and the time is 10-48 h.
  8. 8. The method for preparing heterojunction nano-enzyme according to claim 1, wherein in the step (4), PCC@ZnCdS NPs with different proportions are prepared according to the input molar ratio, the molar amount of Zn (Ac) 2 ·2H 2 O and Cd (Ac) 2 ·2H 2 O is kept unchanged, and seven samples of PZ-0.5, PZ-1.0, PZ-1.5, PZ-2.0, PZ-2.5, PZ-3.0 and PZ-8.0 NPs are respectively obtained by changing the molar amount of the added PCC NPs.
  9. 9. Use of the heterojunction nanoenzyme of claim 1 in the preparation of an anti-inflammatory agent or a medicament.
  10. 10. The use according to claim 9, wherein the anti-inflammatory comprises anti-ulcerative colitis or atherosclerosis.

Description

Heterojunction nano-enzyme and preparation method and application thereof Technical Field The invention belongs to the technical field of nano medicines, and particularly relates to heterojunction nano-enzyme and a preparation method and application thereof. Background Reactive Oxygen Species (ROS) include singlet oxygen (1O2), hydrogen peroxide (H 2O2), hydroxyl radicals (OH) and superoxide anions (O 2·-), overproduction can disrupt redox homeostasis, leading to lipid peroxidation, protein and DNA damage, and thus, the initiation of a variety of inflammatory diseases. Oxidative stress-induced ROS overproduction plays an important role in the pathogenesis of Ulcerative Colitis (UC), exacerbating colonic inflammation. Therefore, inhibition of oxidative stress by scavenging excess ROS is critical for the treatment of UC. Nanoezymes with intrinsic enzyme-like activity are of great interest because of their versatility, ease of preparation and adjustability. In particular, noble metal nanoenzymes, metal oxides, metal Organic Frameworks (MOFs) and carbon-based materials exhibit a variety of mimic enzyme activities. Nanoezymes with similar activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) have been used to eliminate Reactive Oxygen Species (ROS) to achieve cytoprotective, anti-inflammatory or anticancer theranostic. The synthesized gel@Co 3O4 @TGP integrates gastric acid protection, colon targeting, ROS scavenging and natural anti-inflammatory, has obvious advantages of convenience in oral administration, high safety, synergy and the like, and still needs to solve key bottlenecks of release accuracy, drug loading and the like. In the invention, the catalytic center electronic structure can be optimized by regulating and controlling the Co 3O4Co2+/Co3+ proportion, and a new thought is provided for developing the efficient ROS scavenging metal oxide material. NLRP3 inflammatory corpuscles are important members of the NOD-like receptor family, and play a key role in inflammatory and immune responses by promoting maturation and secretion of interleukin-1β (IL-1β) and IL-18, primarily through activation of Caspase-1. Studies have shown that ROS are upstream factors of NLRP3 inflammatory body activation, which plays an important role in colitis. Furthermore, H 2 S has been shown to modulate inflammatory responses and its synthesis increases under pro-inflammatory conditions. There is evidence that H 2 S-supplemented donors may be beneficial in inhibiting NLRP3 inflammatory body activation. In addition, H 2 S has been reported to reduce inflammation in colitis by inhibiting activation of the nuclear factor- κB (NF- κB) pathway or exerting its antioxidant and immunomodulatory effects. Disclosure of Invention Aiming at the problems in the prior art, the invention provides the heterojunction nano-enzyme, and the proportion of Co 2+/Co3+ in the material is successfully improved by constructing the PCC@ZnCdS heterojunction nano-enzyme, so that the ROS scavenging capability is obviously enhanced. The PCC@ZnCdS heterojunction nano enzyme constructed by the invention shows good multi-enzyme activities such as superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx), catalase (CAT) and the like, and the maximization of the antioxidant activity is realized. The mechanism research shows that the material can release endogenous H 2 S, inhibit inflammatory factor release and inhibit NLRP3 inflammatory corpuscle activation. The combination of the two can remove the existing ROS and simultaneously block the continuous generation of the ROS, inhibit the generation of the ROS from the source and relieve the inflammation. The invention also provides a preparation method and application of the heterojunction nano-enzyme. According to the technical scheme, in order to achieve the purpose, the heterojunction nano-enzyme is synthesized into cubic Co 3O4 (PCC NPs) through a hydrothermal method, the molar ratio of the cubic Co 3O4 to a zinc source and a cadmium source is changed, and a series of heterojunction nano-enzymes are synthesized under the conditions of high temperature and high pressure. The heterojunction nano-enzyme is in a cube structure, nano-spheres uniformly grow around the cube, and the size of the heterojunction nano-enzyme is about 230-270 nm. The preparation method of the heterojunction nano-enzyme comprises the following steps: (1) Dissolving Co (NO 3)2·6H2 O and NaOH respectively in deionized water, dropwise adding a NaOH solution into the Co (NO 3)2 solution under stirring, continuously stirring, performing hydrothermal reaction on the mixed solution to obtain a precursor, and calcining the dried precursor to obtain a target product PCC NPs; (2) Dissolving Zn (Ac) 2·2H2 O and Cd (Ac) 2·2H2 O in deionized water, adding Thioacetamide (TAA) after ultrasonic treatment, continuously stirring, dissolving NaOH in the deionized water, pouring the solution into the deionized water, cont