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CN-122005621-A - Application of bifidobacterium adolescentis in improving muscle quality

CN122005621ACN 122005621 ACN122005621 ACN 122005621ACN-122005621-A

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to application of bifidobacterium adolescentis in improving muscle quality. The bifidobacterium adolescentis YBT652 provided by the invention is screened from teenager stool samples in the Harbin region, and has good muscle quality improving capability through verification. The microgel preparation constructed by the bifidobacterium adolescentis YBT652 can not only effectively realize the embedding of the bifidobacterium adolescentis, but also promote the gastrointestinal digestion resistance and the adhesion capability of the bifidobacterium adolescentis in intestinal tracts. In particular, the invention discloses the remarkable effect of the micro gel preparation of the bifidobacterium adolescentis on improving the muscle mass attenuation through d-galactose induction of the muscle mass attenuation and simulation of the aging process, can provide a new thought and method for treating the muscle mass attenuation by microorganisms, and provides technical support and theoretical basis for developing novel therapeutic drugs.

Inventors

  • LV WENQING
  • FAN CHONGHUA
  • LI BAILIANG

Assignees

  • 黑龙江优贝特乳业有限公司
  • 东北农业大学

Dates

Publication Date
20260512
Application Date
20260408

Claims (9)

  1. 1. The preparation method of the micro gel preparation of the bifidobacterium adolescentis is characterized by comprising the following steps of: (1) Amidating pectin to obtain amidated pectin; preparing an amidated pectin solution from the amidated pectin; carrying out grafting reaction on carboxymethyl cellulose and dopamine hydrochloride in the presence of 1-ethyl (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide to obtain sodium carboxymethyl cellulose grafted dopamine hydrochloride; Preparing bifidobacterium adolescentis into a bacterial suspension, wherein the bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacterium adolescentis) YBT652 which is preserved in the Guangdong province microorganism strain preservation center, the preservation number is GDMCC No: 67829, and the preservation date is 2026, 02 and 09 days; (2) The fungus suspension is centrifuged and resuspended in the amidated pectin solution to obtain a binary mixed solution, and the binary mixed solution is mixed with the sodium carboxymethyl cellulose grafted dopamine hydrochloride to obtain a ternary mixed solution; (3) Preparing the ternary mixed solution into liquid drops, and then injecting the liquid drops into a curing solution for curing to obtain the micro gel preparation of the bifidobacterium adolescentis.
  2. 2. The microgel preparation of bifidobacterium adolescentis according to claim 1, wherein in the step (1), the amidation treatment is to stir pectin with an ammonia water-isopropanol mixed solution, the rotation speed of the stirring reaction is 200-400 rpm, the time is 100-240 min, and the concentration of the amidated pectin solution is 2-4% m/V.
  3. 3. The microgel preparation of bifidobacterium adolescentis according to claim 1, wherein in the step (1), the mass ratio of carboxymethyl cellulose, dopamine hydrochloride, 1-ethyl (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide is (2-3) to (2.0-2.8) to (1.2-1.6).
  4. 4. The bifidobacterium adolescentis microgel preparation according to claim 1, wherein in the step (1), the grafting reaction is carried out under the protection of nitrogen, wherein the grafting reaction is carried out at a temperature of 18-26 ℃ for 20-30 hours and at a pH of 4-6.
  5. 5. The microgel preparation of bifidobacterium adolescentis according to claim 1, wherein in step (1), the effective bacterial concentration in the bacterial suspension is (0.5-1.5) ×10 9 CFU/mL.
  6. 6. The bifidobacterium adolescentis microgel preparation according to any one of claims 1-5, wherein in step (2), the volume ratio of the bacterial suspension to the amidated pectin solution is 1: (0.8-1.2).
  7. 7. The bifidobacterium adolescentis microgel preparation according to any of claims 1-5, wherein in step (2), the concentration of the sodium carboxymethyl cellulose grafted dopamine hydrochloride added to the binary mixture is 1-2% m/V.
  8. 8. The bifidobacterium adolescentis microgel preparation according to any of claims 1-5, wherein in step (3), the ternary mixed solution is prepared into droplets by a microfluidic technology, and the solidifying solution is a calcium chloride solution with a concentration of 1-5wt%.
  9. 9. Use of a microgel formulation of bifidobacterium adolescentis as claimed in any of claims 1 to 8 in the manufacture of a medicament for the treatment of muscle mass loss.

Description

Application of bifidobacterium adolescentis in improving muscle quality Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to application of bifidobacterium adolescentis in improving muscle quality. Background Muscle mass attenuation belongs to an age-related disease, and is an senile syndrome in which the muscle mass, content and/or body function of a human body gradually decrease. In addition, in the aged population between 60 and 70 years old, the prevalence of muscle mass attenuation is 5% -13%, and the skeletal muscle atrophy rate of the aged over 80 years old is as high as 11% -50%, so that the health quality and the quality of life of the aged are seriously affected. In humans, progressive mitochondrial dysfunction with aging promotes the production of Reactive Oxygen Species (ROS), which in turn accelerates further mitochondrial deterioration and overall cell damage. In addition, ROS break the balance of protein synthesis and degradation by activating ubiquitin-protease pathway and inhibiting PI3K/Akt/mTOR signaling pathway, ultimately manifesting as enhanced muscle protein catabolism, muscle fiber atrophy and reduced muscle function. Currently, clinical treatment of muscle mass attenuation generally requires comprehensive intervention, mainly by drugs that promote muscle synthesis, improve metabolism, and supplement nutrition to relieve symptoms. However, when the conventional medicine mode is adopted for treatment, the problem of poor metabolism and absorption is easy to occur, and side effects such as edema, muscle pain, abnormal heart rhythm, abnormal urination and the like are also easy to be caused, so that the actual treatment effect is greatly limited. Therefore, it is important to find safer and more effective drugs for the treatment of muscle mass loss. In recent years, along with the vigorous development of microecology, the probiotic preparation has the characteristics of safety, naturalness and no side effect, and can colonize in intestinal tracts and change the flora composition of specific parts of hosts, so that the probiotic preparation is widely paid attention to the medical field. Thus, probiotics are expected to bring new therapeutic regimens and drug options for muscle mass attenuation. However, there is currently no probiotic product with excellent functional properties and which is able to effectively enhance the efficacy of treatments for muscle mass attenuation. Therefore, how to develop a probiotic with remarkable functional activity and related preparation thereof, thereby achieving the purpose of remarkably improving the curative effect of muscle mass attenuation, and becoming a technical problem to be solved in the field. Disclosure of Invention In order to overcome the defects in the prior art, the invention aims to provide the application of bifidobacterium adolescentis in improving the muscle quality. The bifidobacterium adolescentis YBT652 provided by the invention has an excellent effect of improving the muscle quality through verification. The microgel preparation constructed by the bifidobacterium adolescentis YBT652 can not only effectively realize the embedding of the bifidobacterium adolescentis and improve the gastrointestinal tract digestion resistance and the adhesion capability in intestinal tracts, but also can particularly and remarkably enhance the functional characteristics of probiotics in the aspect of treating muscle mass attenuation. In order to achieve the above object, a first aspect of the present invention adopts the following technical solutions: A preparation method of a microgel preparation of bifidobacterium adolescentis comprises the following steps: (1) Amidating pectin to obtain amidated pectin; preparing an amidated pectin solution from the amidated pectin; carrying out grafting reaction on carboxymethyl cellulose and dopamine hydrochloride in the presence of 1-ethyl (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide to obtain sodium carboxymethyl cellulose grafted dopamine hydrochloride; Preparing bifidobacterium adolescentis into a bacterial suspension, wherein the bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacterium adolescentis) YBT652 which is preserved in the Guangdong province microorganism strain preservation center, the preservation number is GDMCC No: 67829, and the preservation date is 2026, 02 and 09 days; (2) The fungus suspension is centrifuged and resuspended in the amidated pectin solution to obtain a binary mixed solution, and the binary mixed solution is mixed with the sodium carboxymethyl cellulose grafted dopamine hydrochloride to obtain a ternary mixed solution; (3) Preparing the ternary mixed solution into liquid drops, and then injecting the liquid drops into a curing solution for curing to obtain the micro gel preparation of the bifidobacterium adolescentis. In the step (1), the amidation treatment is carried ou