CN-122005623-A - Strain for improving human vagina microecology and application thereof

Abstract

The application discloses an application of a strain in producing an external product for improving human vaginal microecology, wherein the strain is Lactobacillus crispatus SPR, has 16S rDNA shown as SEQ ID NO.1, the preservation number is GDMCC NO:66664, the strain produces H 2 O 2 and only D-lactic acid, no drug resistance gene is found, and the external product is suitable for improving the change of human vaginal microecology caused by vaginal pathogenic bacteria. Compared with other lactobacillus crispatus of the same kind, the strain of the application has stronger H 2 O 2 production capability, faster H 2 O 2 production speed, larger total H 2 O 2 production amount in 12 hours, and the concentration of hydrogen peroxide can reach about 220 mu M/L after 12 hours of culture in 1% of conventional inoculum size. H 2 O 2 is a strong oxidant, and can directly kill or inhibit various pathogenic bacteria and conditional pathogenic bacteria such as BV related anaerobic bacteria of Gardnerella, acinetobacter, prevotella and the like.

Inventors

• LI TIYUAN
• MA WEI
• WU YINGJUAN
• Kong Huaxu

Assignees

• 圆美细胞生物科技(深圳)有限公司

Dates

Publication Date

20260512

Application Date

20251108

Claims (8)

1. 1. An application of a strain in the production of an external product for improving human vaginal microecology is characterized in that the strain is Lactobacillus crispatus SPR003 and has 16S rDNA shown as SEQ ID NO. 1; the preservation number is GDMCC NO:66664; The strain produces H 2 O 2 , only D-lactic acid is produced, and no drug resistance gene is found; The external product is suitable for improving the micro-ecological change of human vagina caused by vagina pathogenic bacteria.
2. 2. The use of claim 1, wherein the topical product comprises at least one of a decontaminating agent, a disposable hygienic product, a topical medicament, and a topical medical device.
3. 3. The use according to claim 1, wherein the topical product comprises a gynecological gel product for the adjustment, maintenance and treatment of vaginal microecology in women.
4. 4. The use according to claim 3, wherein the gynaecological gel product comprises a therapeutic gel and a nursing gel.
5. 5. The use of claim 1, wherein the vaginal pathogenic bacteria are anaerobic bacteria, including at least one of the genera prasuvorexa, streptococcus, campylobacter, gardnerella, atopomyces, staphylococcus, candida.
6. 6. The use of claim 5, wherein said prevotella comprises at least one of Prevotella disiens, prevotella buccae, prevotella bivia; The gardnerella comprises at least one of GARDNERELLA LEOPOLDII, GARDNERELLA PIOTII, GARDNERELLA PHOENICIS, GARDNERELLA SWIDSINSKII; The staphylococcus genus includes staphylococcus aureus; The candida genus includes candida albicans.
7. 7. The use according to claim 1, wherein the topical product is suitable for maintaining the pH of the human vaginal microecology between 3.8 and 4.0.
8. 8. The microbial inoculum, wherein the active ingredient of the microbial inoculum comprises the fermentation broth obtained by the strain Lactobacillus crispatus SPR or the fermentation strain Lactobacillus crispatus SPR003 in claim 1.

Description

Strain for improving human vagina microecology and application thereof Technical Field The application relates to the technical field of microorganisms, in particular to a strain for improving human vagina microecology and application thereof. Background The female vagina is composed of inherent anatomy structure, immune system and microecological flora in vagina, and has a large microecological quantity and variety, and is influenced by various factors such as internal factor and external factor, and the dominant bacteria such as lactobacillus can secrete and produce enzyme substances, lactic acid, hydrogen peroxide and the like, so as to maintain ecological balance and acidic environment of vagina to the maximum extent [ Huang Fei, etc. ] high-risk human papillomavirus infection is related to vaginal microecology and cervical local cell immunity [ J ]. J. China journal of microecology, 2022,34 (4): 463-466 ]. The vaginal flora balance is characterized by a dominant microbiota of beneficial lactobacilli. In sharp contrast, vaginal dysbacteriosis, such as bacterial vaginosis BV, is characterized by excessive proliferation of a variety of anaerobic bacteria, which can significantly increase the risk of urogenital and reproductive health problems. The main difference between the state of flora balance and the state of dysbacteriosis is the concentration of lactic acid, namely, the high concentration of lactic acid produced by lactic acid bacteria in the state of balance acidizes the vagina, and the concentration of lactic acid suddenly drops and the pH value rises during the state of dysbacteriosis. When the vaginal microecological balance is broken, the incidence of gynecological diseases, including gynecological tumors, is increased. Cervical cancer is a common gynaecological malignancy, mainly associated with persistent infections with high-risk Human Papillomaviruses (HPV). Recent studies [ Ke Peng, et al mSystems, 2025 Sep 23;10 (9): e0068325. Doi: 10.1128/msystems.00683-25 ] have found that extracellular vesicles (nCEVs) of Lactobacillus crispatus origin are critical for cervical vaginal homeostasis, nCEVs delivers the key bioactive component D-lactic acid, promoting wound healing by macrophage polarization, blocking HPV infection. (remark: mSystems is an open acquisition journal under the American Society of Microbiology (ASM) flag, focused on high-level research in microbiology and systems biology, currently influencing factor 5.0, belonging to the journal of JCR Q1 and the department of China biology 2.) The existing strain for improving the human vagina microecology has insufficient capability of producing H 2O2 under the condition of low strain concentration, has lower total amount of D-lactic acid produced in a short period or lower concentration, has limited killing effect on harmful anaerobic bacteria, and is not suitable for developing word size eliminating products. Content of the application Aiming at the defects in the prior art, the application provides a strain for improving human vagina microecology and application thereof, so as to solve the problems that the existing strain for improving human vagina microecology has insufficient H 2O2 production capability and generates low total amount or low concentration of D-lactic acid in a short period, and is not suitable for developing word size eliminating products. In order to solve the technical problems, the application is realized by the following scheme: an application of a strain in the production of an external product for improving human vaginal microecology is characterized in that the strain is Lactobacillus crispatus SPR003 and has 16S rDNA shown as SEQ ID NO. 1; The strain produces H 2O2, only D-lactic acid is produced, and no drug resistance gene is found; The external product is suitable for improving the micro-ecological change of human vagina caused by vagina pathogenic bacteria. Further, the external product includes at least one of a decontaminating agent, a disposable hygienic product, an external drug, and an external medical device. Further, the external product comprises a gynecological gel product, and is used for adjusting, maintaining and treating female vaginal microecology. Further, the gynecological gel product comprises a therapeutic gel and a nursing gel. Further, the vaginal pathogenic bacteria are anaerobic bacteria including at least one of Prevotella, streptococcus, acinetobacter, gardnerella, altobosa, staphylococcus, and Candida. Further, the Prevotella comprises at least one of Prevotella disiens, prevotella buccae, prevotella bivia; The gardnerella comprises at least one of GARDNERELLA LEOPOLDII, GARDNERELLA PIOTII, GARDNERELLA PHOENICIS, GARDNERELLA SWIDSINSKII; The staphylococcus genus includes staphylococcus aureus; The candida genus includes candida albicans. Still further, the topical product is suitable for maintaining the pH of the human vaginal microecology between 3.8 and 4.0. The application also