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CN-122005633-A - Kiwi berry stem source volatile active ingredient extract and application thereof

CN122005633ACN 122005633 ACN122005633 ACN 122005633ACN-122005633-A

Abstract

The invention belongs to the technical field of component extraction, and particularly relates to a actinidia arguta stem source volatile active component extract and application thereof. The volatile active ingredient extract of the actinidia arguta stem source is obtained by utilizing the salting-out effect of sodium chloride to release the volatile active ingredient in the actinidia arguta stem source and then performing headspace solid-phase microextraction through a DVB/CAR/PDMS three-phase extraction head. The actinidia arguta stem volatile active ingredient extract has remarkable alpha-glucosidase inhibition activity and insulin sensitization, and can synergistically improve insulin resistance and exert hypoglycemic effect through a double mechanism of promoting peripheral glucose consumption and increasing liver glycogen synthesis.

Inventors

  • HOU YUFEI
  • SHE JUNJUN
  • Wang yalong
  • BAI LU
  • BAI NAISHENG

Assignees

  • 西安交通大学第一附属医院榆林医院

Dates

Publication Date
20260512
Application Date
20260407

Claims (9)

  1. 1. The extract of the volatile active ingredients of the actinidia arguta stems with the anti-diabetes effect is characterized in that the volatile active ingredients in the actinidia arguta stems are released by salting out with sodium chloride, and then the volatile active ingredients are obtained by headspace solid-phase microextraction through a DVB/CAR/PDMS three-phase extraction head, wherein the mass ratio of the sodium chloride to the actinidia arguta stems is 1-4:1-3; the temperature of the headspace solid-phase microextraction is 50-60 ℃ and the time is 60-70 min.
  2. 2. The anti-diabetic actinidia arguta stem source volatile active ingredient extract according to claim 1, wherein the relative content of trans-3-hexen-1-ol in the actinidia arguta stem source volatile active ingredient extract is 50.11%, and the relative content of 1-hexanol is 28.39%.
  3. 3. The extract of volatile active ingredients of actinidia arguta stem source with anti-diabetic effect according to claim 1, wherein the actinidia arguta stem source is obtained by subjecting stem source to quick-freezing grinding with liquid nitrogen, and obtaining a sample with particle size less than or equal to 1 mm.
  4. 4. The anti-diabetic actinidia arguta stem source volatile active ingredient extract according to claim 3, wherein the moisture content of the stem source is 70% -80%.
  5. 5. Use of the actinidia arguta stem source volatile active ingredient extract of claim 1 in the preparation of a medicament for alleviating and improving insulin resistance.
  6. 6. The use according to claim 5, wherein the content of volatile active ingredient extract of actinidia arguta stem source in the medicament is not less than 100 μg/mL.
  7. 7. Use of the actinidia arguta stem source volatile active ingredient extract of claim 1 in the preparation of a medicament for inhibiting alpha-glucosidase.
  8. 8. The use according to claim 7, wherein the content of volatile active ingredient extract of actinidia arguta stem source in the medicament is not less than 1mg/mL.
  9. 9. The use according to any one of claims 5 to 8, wherein the volatile active ingredient extract of actinidia arguta stem source is desorbed into a solvent to obtain a desorption solution, or the volatile active ingredient extract of actinidia arguta stem source is adsorbed by a carrier to obtain the medicament.

Description

Kiwi berry stem source volatile active ingredient extract and application thereof Technical Field The invention belongs to the technical field of component extraction, and particularly relates to a actinidia arguta stem source volatile active component extract and application thereof. Background Diabetes, particularly type II diabetes, one of the key pathological mechanisms of type II diabetes is insulin resistance, i.e., the body's reduced sensitivity to insulin, resulting in dysregulation of blood glucose. The current first-line therapeutic drugs comprise biguanides, sulfonylureas and insulin sensitizers, and although the biguanides, sulfonylureas and insulin sensitizers can effectively control sugar, long-term application often accompanies side effects such as gastrointestinal reaction, weight gain, hypoglycemia risk, liver and kidney function injury and the like, and part of the drugs also have drug resistance problems. Therefore, the development of novel high-efficiency low-toxicity insulin sensitizers and alpha-glucosidase inhibitors acting on new targets and having novel action mechanisms, especially derived from natural products, has become an important direction for the current drug development. Actinidia arguta (ACTINIDIA ARGUTA (Siebold & zucc.) plane. Ex miq.) has been described as a medicinal and edible plant in the traditional chinese medicine system for its medicinal value. The composition has sour and astringent stems, and has effects of clearing heat and detoxicating, promoting diuresis, invigorating stomach and tonifying deficiency, and can be used for adjuvant treatment of chronic hepatitis and cancer and traumatic injury. Preliminary research on modern pharmacology proves that the non-volatile components rich in flavonoids, triterpenes and phenolic acids contribute to remarkable anti-tumor, anti-inflammatory, antioxidant and immunity enhancing activities. The prior research shows that the non-volatile extract of the plant extract has definite antidiabetic potential, and lays a foundation for the development of stem resources. However, these studies on non-volatile extracts have focused on the extraction of highly polar components with water or organic solvents. Notably, plant volatile components (VOCs) are not only their characteristic flavors, but are also an important class of active resource libraries. Therefore, further development and research on volatile components thereof are required. Disclosure of Invention In order to solve the problems, the invention provides a actinidia arguta stem source volatile active ingredient extract and application thereof. The invention provides a volatile active ingredient extract of actinidia arguta stems with an anti-diabetes effect, which is obtained by salting out volatile active ingredients in the actinidia arguta stems by using sodium chloride and performing headspace solid-phase microextraction by using a DVB/CAR/PDMS three-phase extraction head, wherein the diameter of the DVB/CAR/PDMS three-phase extraction head is 50/30 mu m, the mass ratio of the sodium chloride to the actinidia arguta stems is 1-4:1-3, and preferably, the mass ratio of the sodium chloride to the actinidia arguta stems is 2:1.5. Based on the salting-out principle, the effect of enhancing the release of volatile components of the sodium chloride aqueous solution is not improved after the sodium chloride aqueous solution is nearly saturated, and excessive solid sodium chloride can adsorb target components and inhibit the release of the target components to the headspace, so that potential damage to an analysis instrument can be caused. Therefore, the mass ratio of the sodium chloride to the stem source is controlled within the range of 1-4:1-3, so that salting-out synergy can be ensured, and adverse effects are avoided; the temperature of the headspace solid-phase microextraction is 50-60 ℃ and the time is 60-70 min. In another preferred embodiment, the relative content of trans-3-hexen-1-ol in the actinidia arguta stem source volatile active ingredient extract is 50.11% and the relative content of 1-hexanol is 28.39%. In another preferred embodiment, the actinidia arguta stem source is obtained by performing quick-freezing grinding on a stem source sample by liquid nitrogen to obtain a sample with the particle size of less than or equal to 1 mm. The treatment embrittles the fibrous structure, increases the specific surface area, and promotes the release of volatile components. In another preferred embodiment, the moisture content of the stem source is 70% -80%, namely the fresh stem source has high moisture characteristic. The invention also provides an application of the actinidia arguta stem source volatile active ingredient extract in preparation of medicines for relieving and improving insulin resistance. The improvement of insulin resistance includes promoting glucose consumption and increasing liver glycogen synthesis in an insulin resistant hepatocyte model. In anoth