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CN-122005635-A - Ginseng extract processing method suitable for industrialized production and capable of selectively improving Rg3 and Rg5 contents

CN122005635ACN 122005635 ACN122005635 ACN 122005635ACN-122005635-A

Abstract

The invention relates to a ginseng extract processing method which is suitable for industrialized production and capable of selectively improving Rg3 and Rg5 contents, and the method comprises the following steps of 1, taking a ginseng extract, diluting the ginseng extract according to the required Rg3 and Rg5 proportion, selecting purified water or 70% alcohol water or 30% alcohol water, preparing a ginseng extract diluted solution, 2, adding the ginseng extract diluted solution into a sealed glass bottle with a cover, adding freshly squeezed lemon juice, putting into a magnetic stirring rotor, fixing the glass bottle into a constant-temperature water bath kettle, stirring for reaction, 3, extracting with water saturated n-butanol, concentrating and drying to obtain a product rich in ginsenoside Rg3 and Rg5 with a fixed proportion. The method can ensure that the content of the ginsenoside Rg3 and the ginsenoside Rg5 is improved, and simultaneously selectively improve the content of a certain saponin, so that the proportion of the ginsenoside Rg3 and the ginsenoside Rg5 is controlled within a reasonable range, which is beneficial to providing customized products for different people, and further improves the curative effect of the products.

Inventors

  • LI LING
  • WANG SHAN
  • CHENG LEQIN
  • WANG RENLONG
  • HAN DA
  • ZHAO SONGYUAN

Assignees

  • 长春中医药大学

Dates

Publication Date
20260512
Application Date
20260126

Claims (6)

  1. 1. A processing method of ginseng extract suitable for industrialized production and capable of selectively improving Rg3 and Rg5 contents is characterized by comprising the following steps: step 1, taking ginseng extract, and diluting the ginseng extract by selecting purified water or 70% alcohol water or 30% alcohol water according to the required ratio of Rg3 to Rg5 to prepare a ginseng extract diluted solution; step 2, adding the ginseng extract diluted solution into a sealed glass bottle with a cover, adding freshly squeezed lemon juice, putting into a magnetic stirring rotor, fixing the glass bottle into a constant-temperature water bath kettle, and reacting under stirring; And 3, after the reaction is finished, extracting with water saturated n-butanol, concentrating and drying to obtain a product rich in ginsenoside Rg3 and Rg5 in a fixed proportion.
  2. 2. The method for preparing a ginseng extract, which is suitable for industrial production and can selectively increase the Rg3 and Rg5 contents, according to claim 1, wherein 1g of ginseng extract is taken, and 25ml of purified water or 70% of alcohol water or 30% of alcohol water is selectively added according to the required ratio of Rg3 and Rg5 to dilute the ginseng extract, so as to prepare a ginseng extract diluted solution.
  3. 3. The method for processing the ginseng extract, which is suitable for industrialized production and can selectively improve the Rg3 and Rg5 contents, according to claim 2, wherein if purified water is adopted for dilution, 100 μl of freshly squeezed lemon juice is added into 1ml of the ginseng extract diluted solution in the step 2, the reaction temperature is controlled between 85 ℃ and 95 ℃, the reaction time is controlled between 3 hours and 4 hours, and the content of ginsenoside Rg3 is higher than the content of ginsenoside Rg5 and is more than or equal to 2 times.
  4. 4. The method for processing the ginseng extract, which is suitable for industrialized production and can selectively improve the Rg3 and Rg5 contents, according to claim 2, wherein if 70% of alcohol water is adopted for dilution, 200-300 μl of freshly squeezed lemon juice is added into 1ml of the ginseng extract diluted solution in the step 2, the reaction temperature is controlled at 75 ℃, the reaction time is controlled at 5-6 hours, the ginsenoside Rg3 content is higher than the ginsenoside Rg5 content and is more than 2 times when the reaction time is 5 hours, and the ginsenoside Rg3 content is less than the ginsenoside Rg5 content when the reaction time is 6 hours.
  5. 5. The method for processing ginseng extract, which is suitable for industrial production and can selectively improve the Rg3 and Rg5 contents, according to claim 2, wherein if 30% alcohol water is adopted for dilution, 300-400 μl of freshly squeezed lemon juice is added into 1ml of ginseng extract diluted solution in step 2, the reaction temperature is controlled at 79 ℃, the reaction time is controlled at 6 hours, and the ginsenoside Rg3 content is controlled to be 1.5-1.7 times higher than the ginsenoside Rg5 content.
  6. 6. The method for processing ginseng extract, which is suitable for industrial production and can selectively increase the content of Rg3 and Rg5, according to claim 2, wherein the method for preparing ginseng extract is as follows: Step 1, taking Yuanshenjing medicinal materials, and carrying out hot reflux extraction by using 75% ethanol to obtain ethanol extract; step 2, draining the dregs after ethanol extraction, transferring to an extraction tank, adding purified water, and performing decoction extraction under the boiling condition of 100 ℃ to obtain water extract; And 3, mixing the ethanol extract obtained in the step 1 and the step 2 with the water extract, and spin-drying to obtain the ginseng extract.

Description

Ginseng extract processing method suitable for industrialized production and capable of selectively improving Rg3 and Rg5 contents Technical Field The invention belongs to the technical field of natural product processing and conversion, and particularly relates to a processing method of a ginseng extract, which is suitable for industrialized production and can selectively improve Rg3 and Rg5 contents. Background Ginsenoside Rg3 and Rg5 are important active secondary saponins in ginseng, and have various pharmacological effects of resisting tumor, enhancing immunity, resisting oxidation and the like. However, these two saponins are very low in natural ginseng. The traditional method is mainly prepared by acid-catalyzed hydrolysis of total saponins of ginseng or protopanaxadiol saponins, but the existing method generally has the problems of low selectivity of target products, difficult control of the ratio of Rg3 to Rg5, severe reaction conditions (possibly involving strong acid and high temperature), complex post-treatment, and the like, has high equipment requirements or has potential safety hazards, and is unfavorable for industrialized application. For example, chinese patent CN 110302225A discloses a red ginseng processing method for improving the contents of ginsenoside Rg3, rg5 and RK1, which comprises the following steps of cleaning ginseng, adding a mixed solution of apple pear juice, lemon juice and water for soaking, wherein the pH value of the mixed solution is 3.0-5.5, the soaking time is 2-3 hours, the surface moisture of the ginseng is drained, steaming is carried out at 105-140 ℃, the steaming time is 3-5 hours, the steamed ginseng is taken out, and the ginseng is dried at 45-65 ℃ for 8-24 hours, so that the red ginseng rich in the ginsenoside Rg3, rg5 and RK1 is obtained. The content of ginsenoside Rg3, rg5 and RK1 of the red ginseng prepared by the method is 20-50 times, 20-30 times and 75-86 times of that of common red ginseng respectively, but the red ginseng is used as a raw material, the amount of juice in the processing process is large, the processing period is long, the preparation cost is high, the industrial production is not facilitated, side reactions are easy to occur under the reaction condition of strong acid and high temperature, the safety of the product cannot be effectively ensured, and in addition, the method cannot reasonably regulate the proportion of Rg3 to Rg5, so that the efficacy of the product is fuzzy, and the method is only suitable for small parts of people. Therefore, it is necessary to develop a mild processing method which is green, efficient, selectively improves the Rg3 and Rg5 contents, is suitable for industrialized amplification landing, and has important significance for the development of functional foods, health-care products and medicines. Disclosure of Invention In view of the disadvantages and shortcomings of the prior art, the invention aims to provide a processing method of ginseng extract, which is suitable for industrialized production and can selectively improve the contents of Rg3 and Rg5, and the method is simple and convenient to operate, mild in condition and capable of efficiently and selectively improving the contents of ginsenoside Rg3 and Rg 5. In order to achieve the above purpose, the invention adopts the following technical scheme: A processing method of ginseng extract suitable for industrialized production, capable of selectively improving Rg3 and Rg5 contents, comprising the following steps: step 1, taking ginseng extract, and diluting the ginseng extract by selecting purified water or 70% alcohol water or 30% alcohol water according to the required ratio of Rg3 to Rg5 to prepare a ginseng extract diluted solution; step 2, adding the ginseng extract diluted solution into a sealed glass bottle with a cover, adding freshly squeezed lemon juice, putting into a magnetic stirring rotor, fixing the glass bottle into a constant-temperature water bath kettle, and reacting under stirring; And 3, after the reaction is finished, extracting with water saturated n-butanol, concentrating and drying to obtain a product rich in ginsenoside Rg3 and Rg5 in a fixed proportion. As a preferred aspect of the present invention, 1g of ginseng extract is taken, and 25ml of purified water or 70% alcohol water or 30% alcohol water is optionally added according to the desired ratio of Rg3 and Rg5 to dilute the ginseng extract, thereby preparing a ginseng extract diluted solution. As the preference of the invention, if purified water is adopted for dilution, 100 mu l of freshly squeezed lemon juice is added into 1ml of ginseng extract dilution solution in the step 2, the reaction temperature is controlled at 85-95 ℃, the reaction time is controlled at 3-4 hours, and the content of ginsenoside Rg3 is higher than that of ginsenoside Rg5 and is more than or equal to 2 times. As the preference of the invention, if 70% alcohol water is adopted for dilution, 200-3