CN-122005638-A - Jerusalem artichoke extract and application thereof in resisting inflammation and regulating intestinal flora and metabolites thereof

Abstract

The invention belongs to the technical field of natural product extraction and biological medicine preparation, and particularly relates to a jerusalem artichoke extract and application thereof in anti-inflammatory and intestinal flora regulation and metabolites thereof. Experiments prove that the jerusalem artichoke extract has the effect of relieving ulcerative colitis. In particular, jerusalem artichoke extract can relieve intestinal structure injury, inflammatory cell infiltration and intestinal barrier injury caused by DSS induction, and has the function of relieving ulcerative colitis. Meanwhile, the invention discloses an anti-inflammatory effect of the jerusalem artichoke extract. In addition, jerusalem artichoke extract has intestinal flora regulating effect. In the technical scheme, the jerusalem artichoke extract obviously regulates the intestinal flora disorder induced by DSS. Jerusalem artichoke extract relieves intestinal dysbacteriosis of DSS induced mice, restores microbial community structure, and regulates metabolites thereof. The jerusalem artichoke extract has the advantages of simple extraction method, low cost, universality and easy mass production, and thus has good practical application value.

Inventors

• FAN YUNPENG
• LI HUICONG
• MA LIN
• LIU YINGQIU

Assignees

• 西北农林科技大学

Dates

Publication Date

20260512

Application Date

20250407

Claims (10)

1. 1. Use of jerusalem artichoke extract in any one or more of the following: (a) Anti-inflammatory or preparing anti-inflammatory products; (b) Regulating the intestinal flora and its metabolites, and/or preparing a product regulating the intestinal flora and its metabolites; (c) Preparing a product for preventing and/or treating ulcerative colitis; Wherein the Jerusalem artichoke extract is ethanol extract of Jerusalem artichoke.
2. 2. The use of claim 1, wherein in said use (a), said anti-inflammatory agent is characterized by in vitro anti-inflammatory and in vivo anti-inflammatory agents, wherein said in vitro anti-inflammatory agent inhibits expression of a pro-inflammatory factor in an inflammatory cell, promotes expression of an anti-inflammatory factor in an inflammatory cell, wherein said pro-inflammatory factor comprises IL-1 beta and IL-6, and said anti-inflammatory factor comprises IL-10; the inflammatory cells are in particular LPS-mediated inflammatory cells; The in vivo anti-inflammatory agents are embodied to alleviate compound-mediated localized swelling, more specifically, to alleviate xylene-mediated ear swelling, and carrageenan-mediated paw swelling.
3. 3. The use according to claim 1, wherein in said use (b) the modulation of intestinal flora and its metabolic disorders is characterized by alleviating DSS-induced disorders of intestinal flora, restoring the structure of the microflora and modulating its metabolites.
4. 4. The use according to claim 1, wherein in said use (c), said preventing and/or treating ulcerative colitis is characterized by alleviation of intestinal structural damage, inflammatory cell infiltration and intestinal barrier damage caused by DSS induction.
5. 5. The use according to claim 1, wherein the products include, but are not limited to, food products, pharmaceutical products and feed products.
6. 6. A method for preparing jerusalem artichoke extract, comprising: Crushing jerusalem artichoke, extracting with ethanol under reflux, concentrating to obtain ethanol extract concentrate, centrifuging, filtering to remove impurities, and drying.
7. 7. The method of claim 6, wherein the ethanol reflux extraction is performed multiple times; further, the specific condition of the ethanol reflux extraction is that the crushed jerusalem artichoke is subjected to reflux extraction with 70-95% ethanol for three times in sequence, wherein the extraction time is 1-3h, and the extraction time is 7-10 times (preferably 10 times, g/mL), 4-8 times (preferably 6 times, g/mL) and 4-8 times (preferably 6 times, g/mL).
8. 8. The preparation method of claim 7, wherein after each ethanol reflux extraction, cooling to room temperature, concentrating under reduced pressure until no ethanol smell exists, centrifuging, removing impurities by suction filtration, heating and concentrating at 60 ℃ until each milliliter is equivalent to 1g of the raw medicinal material, and freeze-drying to obtain the jerusalem artichoke extract; Further, the suction filtration uses a fibrous filter membrane having pore diameters of 0.45 μm and 0.22 μm.
9. 9. A product, characterized in that it comprises a jerusalem artichoke extract, in particular, produced by the preparation method according to any one of claims 6 to 8.
10. 10. The product of claim 9, wherein the product has any one or more of the following efficacy: (a) Anti-inflammatory; (b) Regulating intestinal flora and metabolic disorders thereof; (c) Preventing and/or treating ulcerative colitis; The products include foods, medicines and feeds.

Description

Jerusalem artichoke extract and application thereof in resisting inflammation and regulating intestinal flora and metabolites thereof Technical Field The invention belongs to the technical field of natural product extraction and biological medicine preparation, and particularly relates to a jerusalem artichoke extract and application thereof in anti-inflammatory and intestinal flora regulation and metabolites thereof. Background The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art. Ulcerative colitis (Ulcerative Colitis, UC) is a chronic nonspecific inflammatory bowel disease, the pathogenesis of which is not clear, and which may be related to genetic, immune, environmental, infection and other factors, and has a family genetic history, good onset for long-term smokers and high-fat diets, and low illness and death rate. Clinical symptoms include diarrhea, abdominal pain, mucopurulent bloody stool, and the like, without infectivity, severe complications such as intestinal perforation, massive hemorrhage, canceration, and the like. The treatment of the disease mainly comprises drug treatment, and the treatment of the drug is ineffective or complicated with serious complications and canceration needs to be considered during the treatment of the operation. The disease has the characteristics of repeated attack and alternate alleviation, and seriously affects the life quality of patients, but when the disease is serious or complications occur, the life can be endangered. Maintaining a healthy lifestyle, such as regular work and rest, balanced diet and moderate exercise, smoking cessation and alcohol limitation, regular physical examination, and timely discovery and treatment of possible complications are key to preventing the disease. Jerusalem artichoke is sweet in taste, slightly bitter and cool in nature, and is suitable for some diseases requiring heat clearing and blood cooling in treatment. Research shows that Jerusalem artichoke has effects of reducing blood lipid and blood sugar, resisting oxidation, resisting cancer, resisting bacteria, enhancing immunity, etc., and can be used as antipyretic, analgesic, antiinflammatory and antispasmodic medicine for treating fracture, swelling, skin wound and pain, and treating intestinal diseases. However, there are few reports in the prior art on the use of jerusalem artichoke extract for non-specific inflammatory diseases such as ulcerative colitis. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a jerusalem artichoke extract and application thereof in anti-inflammatory and intestinal flora regulation and metabolites thereof. Specifically, experimental researches show that the jerusalem artichoke extract is extracted from jerusalem artichoke by adopting an alcohol extraction mode. Based on the above results, the present invention has been completed. In order to achieve the technical purpose, the invention adopts the following technical scheme: in a first aspect of the invention, there is provided the use of a jerusalem artichoke extract in any one or more of the following: (a) Anti-inflammatory; (b) Regulating intestinal flora and metabolic disorders and; (c) And preparing a product for preventing and/or treating ulcerative colitis. Wherein the Jerusalem artichoke extract is ethanol extract of Jerusalem artichoke. In a second aspect of the present invention, there is provided a method for preparing a jerusalem artichoke extract, the method comprising: Crushing Jerusalem artichoke, reflux extracting with ethanol, concentrating, removing impurities, and drying. In a third aspect of the present invention, there is provided a product comprising the above jerusalem artichoke extract, in particular, the jerusalem artichoke extract being prepared by the preparation method of the second aspect. The product has any one or more of the following effects: (a) Anti-inflammatory; (b) Regulating intestinal flora and metabolic disorders thereof; (c) Preventing and/or treating ulcerative colitis. In the present invention, the products include, but are not limited to, foods, medicines, and feeds. The beneficial technical effects of one or more of the technical schemes are as follows: The technical scheme provides the jerusalem artichoke extract and the specific extraction preparation method thereof, and experiments prove that the jerusalem artichoke extract has the effect of relieving ulcerative colitis. In particular, jerusalem artichoke extract can relieve intestinal structure injury, inflammatory cell infiltration and intestinal barrier injury caused by DSS induction, and has the function of relieving ulcerative colitis. Meanwhile, the technical scheme discloses an anti-inflammatory effect of