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CN-122005649-A - Sophora japonica extract for inhibiting oral biofilm formation and application thereof

CN122005649ACN 122005649 ACN122005649 ACN 122005649ACN-122005649-A

Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to a sophora flower extract for inhibiting oral cavity biological film formation and application thereof. According to the invention, the flos sophorae extract obtained after the ethanol extraction is subjected to specific macroporous resin column elution conditions, the flos sophorae extract obtained through enrichment has excellent function of inhibiting oral cavity biomembrane formation, and the effect of the flos sophorae extract is further improved through further elution under specific silica gel column conditions. The inhibition rate of the obtained pagodatree flower extract is obviously higher than that of pagodatree flower extracts prepared by the traditional alcohol extraction or other processes, and the pagodatree flower extract has important application value in preparing products for inhibiting and/or killing oral microorganisms or biological films thereof, and can be used for preventing and/or treating dental bacterial diseases including decayed teeth.

Inventors

  • YU YI
  • GAO YAN
  • XIAO LEI
  • ZHOU YONG

Assignees

  • 无限极（中国）有限公司

Dates

Publication Date
20260512
Application Date
20251229

Claims (10)

  1. 1. The sophora flower extract is characterized by being prepared by treating extract prepared by extracting sophora flower with alcohol by using nonpolar macroporous resin, eluting with 5-8 vol% ethanol water solution for the first time after full adsorption, eluting with 20-30 vol% ethanol water solution for the second time, collecting eluent obtained by eluting for the second time, concentrating and drying to obtain the sophora flower extract.
  2. 2. The pagodatree flower extract is characterized by mainly comprising the following steps of: (1) Treating the extract prepared by ethanol extraction of the pagodatree flower by using nonpolar macroporous resin, eluting with 5-8 vol% ethanol water solution for the first time to remove impurities after full adsorption, eluting with 20-30 vol% ethanol water solution for the second time, collecting eluent obtained by eluting for the second time, concentrating and drying to obtain the eluting part of the pagodatree flower macroporous resin; (2) Eluting the macroporous resin elution part of the pagodatree flower by a silica gel column, eluting the macroporous resin elution part of the pagodatree flower by a chloroform-methanol-water solution with the volume ratio of (85-95): 5-10): 1 for the first time to remove impurities, eluting the macroporous resin elution part of the pagodatree flower by a methanol-water solution with the volume ratio of (1-2): 1 for the second time, collecting an eluent obtained by the second elution, concentrating and drying to obtain the pagodatree flower extract.
  3. 3. The sophora flower extract according to claim 1 or 2, wherein the nonpolar macroporous resin treatment comprises one or more of the following (1) - (4): (1) The nonpolar macroporous resin comprises any one of a D101 macroporous resin column, an HPD-100 macroporous resin column and an HP-20 macroporous resin column; (2) The time of the sufficient adsorption is 2-4 hours; (3) The eluent used for eluting impurities for the first time is 3-5 times of column volume; (4) The eluent used in the second elution is 3-5 times of column volume.
  4. 4. The sophora flower extract according to claim 2, wherein the conditions for eluting the silica gel column include one or more of the following (1) - (5): (1) The mode of applying the silica gel column is dry method column application; (2) The mass ratio of the macroporous resin elution part of the pagodatree flower to the silica gel is 1 (20-40); (3) The mesh number of the silica gel filled in the silica gel column is 200-300 meshes; (4) The eluent used for eluting impurities for the first time is 3-5 times of column volume; (5) The eluent used in the second elution is 3-5 times of column volume.
  5. 5. The sophora flower extract according to claim 1 or 2, wherein the preparation method of the extract prepared by extracting sophora flower with ethanol comprises the steps of fully extracting sophora flower with 50-70 vol% ethanol water solution, combining the extracting solutions, and concentrating to obtain the extract.
  6. 6. Use of the sophora flower extract according to any one of claims 1-5 for the preparation of a product for inhibiting and/or killing oral microorganisms or their biofilm.
  7. 7. The use according to claim 6, wherein the oral microorganisms comprise one or more of streptococcus mutans, porphyromonas gingivalis, staphylococcus aureus, candida albicans, praecox intermedia, actinomyces naeslundii, actinomyces viscosus, actinomyces griseus, actinomyces odontoseisis, and rochanteria caries.
  8. 8. A product for inhibiting and/or killing oral microorganisms or biofilms thereof, characterized in that the active ingredient of the product comprises one or more of the pagodatree flower extracts of any one of claims 1-5.
  9. 9. Use of the sophora flower extract according to any one of claims 1-5 or the product according to claim 8 for preparing a medicament for preventing and/or treating dental bacterial diseases.
  10. 10. The use according to claim 9, wherein the dental bacterial disease comprises one or more of caries, pulpitis, periodontitis, periapical periodontitis, pericoronitis of wisdom teeth.

Description

Sophora japonica extract for inhibiting oral biofilm formation and application thereof Technical Field The invention belongs to the technical field of biological medicine, and particularly relates to a sophora flower extract for inhibiting oral cavity biological film formation and application thereof. Background The pagodatree flower is used as the dried flower and flower bud of the pagodatree flower of the leguminous plant, has the characteristics of both medicine and food, has the effects of cooling blood, stopping bleeding, clearing liver and purging fire, has pleasant smell and high safety, and is widely applied to various toothpastes and oral care products. For example, chinese patent application discloses a chinese herbal dentifrice containing pagodatree flower extract, which is claimed to be useful for preventing and treating periodontitis, gingivitis, tooth decay, oral mucosal inflammation, halitosis, and other oral problems. However, the pagodatree flower extract adopted by the dentifrice is obtained only through simple alcohol extraction and extraction processes, and the pagodatree flower is only one of compound ingredients, so that the effective active ingredient content is low, and the sufficient efficacy is difficult to be exerted. Furthermore, the patent application does not provide any experimental data to support its claimed control effect, so that its practical application effect lacks scientific basis. At present, the evaluation of the antibacterial effect of traditional Chinese medicines is mostly focused on planktonic bacteria, and common methods comprise determination of the diameter of a bacteriostasis zone, determination of the Minimum Inhibitory Concentration (MIC) and determination of the Minimum Bactericidal Concentration (MBC). However, pathogenic bacteria in the oral cavity mainly exist in a biological film state, and physiological characteristics, drug resistance and pathogenic mechanisms of the pathogenic bacteria are substantially different from those of plankton （Gao Z, Chen X, Wang C, et al. New strategies and mechanisms for targeting Streptococcus mutans biofilm formation to prevent dental caries: a review[J]. Microbiological Research, 2023: 127526.）., so that the antibacterial traditional Chinese medicine extract screened based on plankton models is difficult to effectively inhibit the formation of the biological film of the oral cavity in practical application. The existing oral care products based on the pagodatree flower cannot be optimized aiming at the characteristics of the biological film in terms of component preparation, activity content and action mechanism, so that the control effect on the oral biological film related diseases is limited. In summary, there is an important and urgent need to develop an oral care product that can inhibit the formation of oral biofilms with high efficacy. Disclosure of Invention The invention aims to overcome the defects and shortcomings that the conventional oral product in the prior art has limited inhibition effect on oral biofilms and can not effectively remove oral microorganisms in the oral biofilms, and mainly aims to provide the sophora flower extract capable of effectively inhibiting the oral biofilms, and the inhibition rate on the oral biofilms is higher (more than 84%). It is another object of the present invention to provide the use of the extract of Sophora japonica. It is another object of the present invention to provide a product that inhibits and/or kills oral microorganisms or their biofilm. Another object of the present invention is to provide the use of the extract of Sophora japonica or the product thereof in preparing a medicament for preventing and/or treating dental bacterial diseases The above object of the present invention is achieved by the following technical scheme: The invention provides a sophora flower extract, which is prepared by treating extract prepared by extracting sophora flower with alcohol by using nonpolar macroporous resin, eluting with 5-8 vol% ethanol aqueous solution for the first time after full adsorption, eluting with 20-30 vol% ethanol aqueous solution for the second time, collecting eluent obtained by eluting for the second time, concentrating and drying to obtain the sophora flower extract. The invention provides a pagodatree flower extract, which is prepared by the following steps: (1) Treating the extract prepared by ethanol extraction of the pagodatree flower by using nonpolar macroporous resin, eluting with 5-8 vol% ethanol water solution for the first time to remove impurities after full adsorption, eluting with 20-30 vol% ethanol water solution for the second time, collecting eluent obtained by eluting for the second time, concentrating and drying to obtain the eluting part of the pagodatree flower macroporous resin; (2) Eluting the macroporous resin elution part of the pagodatree flower by a silica gel column, eluting the macroporous resin elution part of the pagodatree