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CN-122005655-A - Magnetic nano-drug carrier

CN122005655ACN 122005655 ACN122005655 ACN 122005655ACN-122005655-A

Abstract

The invention belongs to the technical field of drug carriers, and particularly relates to a magnetic nano drug carrier which is prepared by selecting traditional Chinese medicine raw materials and auxiliary raw materials and corresponding equipment for processing the raw materials, preprocessing the traditional Chinese medicine raw materials, extracting active ingredients through ultrasonic and reflux combination, purifying and concentrating extract, and preparing the extract by a coprecipitation method The application prepares the flexible traditional Chinese medicine dressing by taking the anti-inflammatory analgesic, blood circulation promoting and blood stasis removing active ingredients of 19 traditional Chinese medicines as cores and combining the local magnetic response enrichment characteristic of the magnetic nano carrier, and the flexible traditional Chinese medicine dressing is used by being attached to the wrist strap pressurizing silica gel pad after PCI operation, and the magnetic carrier promotes the local directional enrichment of the active ingredients of the traditional Chinese medicines on the wound, plays the synergistic effects of anti-inflammatory analgesic, microcirculation improvement and vein relaxing and restoring, and solves the clinical pain points of wrist puncture wound inflammation edema, obvious pain and unsmooth local blood circulation after PCI operation, and simultaneously adapts to the flexible, breathable and attaching requirements of the pressurizing silica gel pad.

Inventors

  • Hui Xiaoshan
  • ZHANG ZHENPENG
  • JIANG ZHILIN
  • WANG SHIQI
  • ZHANG JINSHENG
  • XIE ZICONG
  • SUN XUAN
  • LIU LANCHUN
  • HOU CHENGZHI

Assignees

  • 中国中医科学院广安门医院

Dates

Publication Date
20260512
Application Date
20251229

Claims (7)

  1. 1. The preparation method of the magnetic nano-drug carrier is characterized by comprising the following steps of: Firstly, selecting traditional Chinese medicine raw materials and auxiliary raw materials, and selecting corresponding equipment for processing the raw materials; step two, preprocessing the traditional Chinese medicine raw materials, extracting effective components through ultrasonic and reflux combination, and finally purifying and concentrating the extracting solution; step three, preparing the catalyst by a coprecipitation method ; Step four, carrying out surface modification on the active ingredient load and the carrier of the traditional Chinese medicine; Preparing the traditional Chinese medicine magnetic nano dressing into a flexible, breathable and attached dressing; And step six, performing quality control and performance verification on the dressing.
  2. 2. The magnetic nano-drug carrier according to claim 1, wherein in the first step, the traditional Chinese medicine raw materials comprise 30g of native copper, 30g of garden balsam stem, 15g of lycopodium clavatum, 30g of lycopodium clavatum, 15g of teasel root, 10g of frankincense, 10g of myrrh, 15g of ligusticum wallichii, 15g of angelica sinensis, 9g of earthworm, 15g of cortex lycii radicis, 12g of sappan wood, 6g of rhizoma corydalis, 9g of pseudo-ginseng, 9g of asarum, 20g of red sage root, 20g of mulberry twig, 20g of pine branch and 6g of ground beetle; auxiliary materials comprise 、 25% Ammonia water, medical silicone gel, sodium carboxymethyl cellulose, glycerol, polyethylene glycol, medical breathable non-woven fabric, 75% absolute ethyl alcohol, ultrapure water and a 0.45 mu m filter membrane; the corresponding equipment comprises a traditional Chinese medicine pulverizer, an ultrasonic extractor, a rotary evaporator, a high-pressure reaction kettle, a centrifuge, a Transmission Electron Microscope (TEM), a Vibrating Sample Magnetometer (VSM), a High Performance Liquid Chromatograph (HPLC), a dressing forming machine and an ultraviolet-visible spectrophotometer.
  3. 3. The magnetic nano-drug carrier according to claim 1, wherein the detailed process of the second step comprises pulverizing Pyritum, soaking in 5% diluted hydrochloric acid for 30min, removing surface impurities, washing with distilled water to neutrality, oven drying Olibanum and Myrrha at 60deg.C, removing oil, pulverizing Notoginseng radix, lumbricus and Eupolyphaga Seu Steleophaga, sieving with 100 mesh sieve, mixing, pulverizing the rest Chinese medicinal materials, sieving with 80 mesh sieve to obtain Chinese medicinal mixed powder; Mixing the above materials, adding 75% absolute ethanol 10 times of the volume, soaking for 2 hr, ultrasonic extracting at 60deg.C and 200W for 30min, transferring to reflux device, reflux extracting at 80deg.C for 2 hr, filtering to collect the first extractive solution, adding 75% absolute ethanol 8 times of the volume of the residue, reflux extracting for 1 time, mixing the two extractive solutions, vacuum filtering with 0.45 μm filter membrane to remove solid impurities, concentrating the filtrate in rotary evaporator to remove ethanol smell to obtain Chinese medicinal extract, adding 5 times of ultra-pure water, stirring for dissolving, subjecting to AB-8 macroporous adsorbent resin column chromatography, loading at flow rate of 2 BV/hr, eluting with distilled water, eluting with 70% ethanol to obtain eluent, concentrating to obtain Chinese medicinal active ingredient concentrate with concentration of 80mg/mL, and refrigerating at 4deg.C.
  4. 4. The magnetic nano-drug carrier according to claim 1, wherein the detailed procedure of the third step is as follows Molar ratio=1:2, weigh 2.78g、 7.02G, adding 200mL of ultrapure water, stirring in a water bath at 60 ℃ under the protection of nitrogen until the solution is completely dissolved to form a brown yellow uniform solution, slowly dripping 25% ammonia water, regulating the pH of the solution to 9-10, quickly changing the solution into black suspension, continuing stirring and reacting for 1h, adsorbing black precipitate by an external magnet, removing supernatant, washing with ultrapure water for 3 times, washing with absolute ethyl alcohol for 2 times, removing chloride ions and residual ammonia water, vacuum drying at 60 ℃ for 12h, and grinding to obtain the product Magnetic nano powder.
  5. 5. The magnetic nano-drug carrier according to claim 1, wherein the detailed procedure of the fourth step is as follows, 0.5g is weighed Adding 100mL of ultrapure water into the nano powder, performing ultrasonic dispersion for 30min to form uniform suspension, and mixing according to the following steps Nanoparticle, namely, the mass ratio of the active ingredients of the traditional Chinese medicine=1:0.4, slowly adding 40mL of concentrated solution of the active ingredients of the traditional Chinese medicine, magnetically stirring at 25 ℃ for reaction for 4 hours, magnetically separating the magnet, discarding the upper layer of unsupported traditional Chinese medicine solution, collecting the supported magnetic nanoparticle, washing with ultrapure water for 2 times, adding 50mL of ultrapure water into the particle, ultrasonically dispersing for 20 minutes, adding 0.1g of PEG-2000,50 ℃ for stirring for reaction for 2 hours, and enabling PEG molecules to react with the particles through hydroxyl groups And (3) combining surface hydroxyl groups, improving biocompatibility and dispersibility, magnetically separating again, washing with ultrapure water for 3 times, and vacuum drying at 60 ℃ for 8 hours to obtain the traditional Chinese medicine magnetic nano-carrier.
  6. 6. The magnetic nano-drug carrier according to claim 1, wherein the detailed procedure of the fifth step is as follows, 10g of medical silicone gel is taken, 2g of glycerin, 1g of CMC-Na and 50mL of ultrapure water are added, stirring is carried out uniformly, a flexible gel matrix is formed, 0.3g of traditional Chinese medicine magnetic nano-carrier is weighed, the traditional Chinese medicine magnetic nano-carrier is added into the gel matrix, ultrasonic dispersion is carried out for 20min, uniform dispersion and no agglomeration are ensured, the mixed gel is uniformly smeared on medical breathable non-woven fabrics, the medical breathable non-woven fabrics are put into a dressing forming machine, drying is carried out for 12h under the conditions of 37 ℃ and 50% humidity, and a flexible traditional Chinese medicine magnetic nano-dressing is formed, the dressing is cut according to the size of a PCI wrist strap silica gel pad, and sterile packaging is carried out for standby.
  7. 7. The magnetic nano-drug carrier according to claim 1, wherein the detailed steps of the step six are as follows, whether the morphology of the magnetic carrier is spherical and uniformly dispersed is observed through a TEM, whether the particle size is between 30 and 100nm is determined by DLS, whether the zeta potential is between-15 and-8 mV is determined by VSM, whether the saturation magnetization is not less than 40emu/g is determined by VSM, the content of tanshinone IIA, notoginsenoside R1 and tetrahydropalmatine in the dressing is determined by HPLC, the loading rate and the encapsulation rate are calculated, the cumulative release rate is determined by a dialysis bag method for 24 hours, and the cumulative release rate is determined for 48 hours, so that the slow release characteristic is presented.

Description

Magnetic nano-drug carrier Technical Field The invention relates to the technical field of drug carriers, in particular to a magnetic nano drug carrier. Background The magnetic nano-drug carrier is an intelligent drug delivery system combining nano-technology and magnetic response characteristics. The core principle is that therapeutic molecules such as chemotherapeutic drugs, gene drugs or immune regulators are loaded on magnetically responsive nano particles, and targeted delivery, controllable release and cooperative treatment of the drugs are realized by means of external magnetic field guidance and focus microenvironment response. The technology is expected to overcome the limitations of systemic distribution, poor targeting, large side effect, insufficient curative effect and the like of the traditional medicine. However, the existing magnetic nano-drug carriers still have obvious defects. The current research is focused on loading single pharmaceutical ingredients (such as flavonoid compounds like quercetin or curcumin), and the treatment focus and the function are relatively limited. Especially when dealing with the complex clinical problems of PCI postoperative wrist puncture wound, the single drug carrier is difficult to comprehensively meet the comprehensive treatment demands of wound repair, inflammatory edema regression, pain relief and local microcirculation improvement. Therefore, aiming at the multi-ring pathophysiological characteristics of PCI postoperative wound healing, a multifunctional composite magnetic nano-drug carrier capable of synergistically resisting inflammation, promoting repair and improving blood circulation is developed, and the multifunctional composite magnetic nano-drug carrier has important clinical significance and research value. Disclosure of Invention The present invention is directed to a magnetic nano-drug carrier, which solves the problems set forth in the background art. In order to achieve the purpose, the invention provides the following technical scheme that the magnetic nano-drug carrier is prepared by the following steps: Firstly, selecting traditional Chinese medicine raw materials and auxiliary raw materials, and selecting corresponding equipment for processing the raw materials; step two, preprocessing the traditional Chinese medicine raw materials, extracting effective components through ultrasonic and reflux combination, and finally purifying and concentrating the extracting solution; step three, preparing the catalyst by a coprecipitation method ; Step four, carrying out surface modification on the active ingredient load and the carrier of the traditional Chinese medicine; Preparing the traditional Chinese medicine magnetic nano dressing into a flexible, breathable and attached dressing; And step six, performing quality control and performance verification on the dressing. Preferably, in the first step, the traditional Chinese medicine raw materials comprise 30g of native copper, 30g of garden balsam stem, 15g of common clubmoss herb, 30g of common clubmoss herb, 15g of himalayan teasel root, 10g of frankincense, 10g of myrrh, 15g of szechuan lovage rhizome, 15g of Chinese angelica, 9g of earthworm, 15g of cortex acanthopanacis, 12g of sappan wood, 6g of rhizoma corydalis, 9g of pseudo-ginseng, 9g of asarum, 20g of red sage root, 20g of mulberry twig, 20g of pine branch and 6g of ground beetle; auxiliary materials comprise 、25% Ammonia water, medical silicone gel, sodium carboxymethyl cellulose, glycerol, polyethylene glycol, medical breathable non-woven fabric, 75% absolute ethyl alcohol, ultrapure water and a 0.45 mu m filter membrane; the corresponding equipment comprises a traditional Chinese medicine pulverizer, an ultrasonic extractor, a rotary evaporator, a high-pressure reaction kettle, a centrifuge, a Transmission Electron Microscope (TEM), a Vibrating Sample Magnetometer (VSM), a High Performance Liquid Chromatograph (HPLC), a dressing forming machine and an ultraviolet-visible spectrophotometer. Preferably, the detailed process of the second step comprises pulverizing Pyritum, soaking in 5% diluted hydrochloric acid for 30min, removing surface impurities, washing with distilled water to neutrality, oven drying Olibanum and Myrrha at 60deg.C, and removing oil; Mixing the above materials, adding 75% absolute ethanol 10 times of the volume, soaking for 2 hr, ultrasonic extracting at 60deg.C and 200W for 30min, transferring to reflux device, reflux extracting at 80deg.C for 2 hr, filtering to collect the first extractive solution, adding 75% absolute ethanol 8 times of the volume of the residue, reflux extracting for 1 time, mixing the two extractive solutions, vacuum filtering with 0.45 μm filter membrane to remove solid impurities, concentrating the filtrate in rotary evaporator to remove ethanol smell to obtain Chinese medicinal extract, adding 5 times of ultra-pure water, stirring for dissolving, subjecting to AB-8 macroporous adsorbent re