CN-122005658-A - Rhubarb-red sage root self-assembled nano particle and application

Abstract

The invention discloses rheum officinale-red sage root self-assembled nano particles and application thereof. The self-assembled nanoparticles are separated from ethanol extract of the rhubarb and red ginseng compound by means of differential centrifugation, impurity removal and the like. The average particle diameter of the nanoparticle is 500 nm, and Zeta potential is The structural integrity of the nano particles is maintained under the conditions of-80 ℃ and pH 2.2 and pH7.4, and the nano particles have good low-temperature stability and acid-base stability; the cisplatin-induced mouse acute kidney injury model shows that the rhubarb-red sage root nano self-assembled particles have good prevention effect on acute kidney injury, and can be widely applied to preparation of medicines for preventing acute kidney injury.

Inventors

• ZHAO LING
• LIU YANQING
• WANG TING
• LI CHENGHONG
• YANG JING
• Tang huaqiao
• HUANG SIYANG
• YE GANG
• SHI FEI
• ZHU MAIXUN
• XIA XUE

Assignees

• 四川农业大学
• 重庆市畜牧科学院

Dates

Publication Date

20260512

Application Date

20260320

Claims (4)

1. 1. A self-assembled nano-particle of rhubarb and red sage root is prepared from the alcohol extract of rhubarb and red sage root through concentrating, differential centrifugal separation and removing impurities, and hydrogen bonding Self-assembled by stacking, with average particle diameter of 500 nm and zeta potential of And the stability of the nanoparticle structure is maintained under the conditions of-80 ℃ and pH 2.2 and pH 7.4.
2. 2. The rheum officinale-red sage root self-assembled nanoparticle according to claim 1, wherein the preparation method of the nanoparticle comprises the following steps: S1, respectively weighing 20 g parts of rhubarb and red sage root decoction pieces according to the proportion of 1:1, crushing, sieving with a 40-mesh sieve to obtain uniform medicinal powder, adding 10 times of 70% ethanol, soaking at room temperature for 1:1 h, reflux-extracting for 3: 3 h, filtering, repeatedly extracting filter residues with 70% ethanol for 1 time by the same method, filtering, combining 2 times of filtrates, mixing, and concentrating under reduced pressure by a rotary evaporator to obtain a solution of the rhubarb-red sage root extract RSE containing 0.2 g crude drugs/mL; S2, centrifuging RSE at 3000 rpm for 10: 10 min to remove larger particles in the solution, centrifuging the supernatant at 15000 for rpm at 4 ℃ for 40: 40 min, discarding the supernatant after centrifugation, redispersing the precipitate in deionized water, repeating three centrifugation-resuspension cycles to remove residual impurities, filtering the suspension by a 1-mu m needle filter to remove potential aggregates, and freeze-drying to obtain the rheum officinale-red sage root self-assembled nano particles RSNPs.
3. 3. The self-assembled nanoparticle of rhubarb-red sage root according to claim 2, wherein in S2, the freeze-drying is 72 h under the conditions of-50 ℃ and 0.01 MPa.
4. 4. Use of self-assembled nanoparticles of rhubarb-red sage root as defined in any one of claims 1-3 for the prevention of acute kidney injury.

Description

Rhubarb-red sage root self-assembled nano particle and application Technical Field The invention relates to the field of traditional Chinese medicine nano-medicines, in particular to rheum officinale-red sage root self-assembled nano-particles and application thereof in preventing acute kidney injury. Background Acute Kidney Injury (AKI) is a clinical critical condition, and current treatments are mainly supported by symptomatic support, lacking specific repair drugs. The existing rhubarb and red sage root preparations have definite kidney protecting effect, but the bioavailability of the active ingredients is low and kidney targeting is lacking. The traditional Chinese medicine nano delivery technology relies on exogenous carrier materials, has potential safety hazards and loses the synergistic advantage of traditional Chinese medicine compatibility. Although self-assembly strategies have been explored in the field of traditional Chinese medicine, research on constructing a carrier-free and kidney-targeted nano delivery system by co-assembly of rhein and salvianic acid through intermolecular forces is not seen, and the technology in the field is still blank. Disclosure of Invention The invention aims to solve the problems in the prior art and provides a rheum officinale-red sage root self-assembled nanoparticle and application thereof in prevention of acute kidney injury. Based on intermolecular hydrogen bond and pi-pi stacking effect of active components of rheum officinale-red sage root, self-assembled carrier-free nanoparticles with higher purity are separated from ethanol extract of rheum officinale-red sage root by a simple centrifugal separation process, symbiotic delivery and synergistic effect of the active components of the traditional Chinese medicine compound are realized, stability and absorption efficiency of the active components are improved, and the prevention effect on acute kidney injury is further enhanced. The technical scheme adopted by the invention is as follows: A self-assembled nanoparticle of radix et rhizoma Rhei-Saviae Miltiorrhizae radix is prepared from ethanol extract of radix et rhizoma Rhei-Saviae Miltiorrhizae radix compound by concentrating, separating with differential centrifugation method, and removing impurities, wherein the nanoparticle is prepared by hydrogen bonding and Self-assembled by stacking, with average particle diameter of 500 nm and zeta potential ofAnd the stability of the nanoparticle structure is maintained under the conditions of-80 ℃ and pH 2.2 and pH 7.4. Preferably, the preparation method of the nanoparticle comprises the following steps: S1, respectively weighing 20 g parts of rhubarb and red sage root decoction pieces according to the proportion of 1:1, crushing, sieving with a 40-mesh sieve to obtain uniform medicinal powder, adding 10 times of 70% ethanol, soaking at room temperature for 1:1 h, reflux-extracting for 3: 3 h, filtering, repeatedly extracting filter residues with 70% ethanol for 1 time by the same method, filtering, combining 2 times of filtrates, mixing, and concentrating under reduced pressure by a rotary evaporator to obtain a solution of the rhubarb-red sage root extract RSE containing 0.2 g crude drugs/mL; S2, centrifuging RSE at 3000 rpm for 10: 10 min to remove larger particles in the solution, centrifuging the supernatant at 15000 for rpm at 4 ℃ for 40: 40 min, discarding the supernatant after centrifugation, redispersing the precipitate in deionized water, repeating three centrifugation-resuspension cycles to remove residual impurities, filtering the suspension by a 1-mu m needle filter to remove potential aggregates, and freeze-drying to obtain the rheum officinale-red sage root self-assembled nano particles RSNPs. Preferably, in the step S2, the freeze-drying is carried out at-50 ℃ and 0.01MPa for 72 h. The invention also provides application of the rheum officinale-red sage root self-assembled nano-particles in preparing medicines for preventing acute kidney injury. The invention has the beneficial effects that: 1. The self-assembled nano particles in the ethanol extract of the rhubarb and red ginseng compound are separated by low-speed and low-temperature high-speed centrifugation, impurity removal and other modes. The average particle diameter of the nanoparticle is 500 nm, the Zeta potential is-27.59 +/-6.19 mV, and the structural integrity of the nanoparticle is maintained under the conditions of-80 ℃ and pH 2.2 and pH7.4, so that the nanoparticle has low-temperature stability and good acid-base stability. 2. The self-assembled nano-particle of rhubarb-red sage root in the invention is identified by UPLC-MS component analysis that RSNPs contains 101 different phytochemicals. The cisplatin-induced mouse acute kidney injury model shows that the rhubarb-red sage root nano self-assembled particles have good prevention effect on acute kidney injury, obviously reduce the content of urea nitrogen (BUN) and creatinine (Scr)