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CN-122005659-A - Pharmaceutical composition for treating burns and scalds in intensive care and preparation method thereof

CN122005659ACN 122005659 ACN122005659 ACN 122005659ACN-122005659-A

Abstract

The invention provides a pharmaceutical composition for treating burns and scalds in intensive care and a preparation method thereof, and belongs to the technical field of medicines. Comprises the following raw materials, by weight, 1-1.5 parts of analgesic temperature response liposome, 0.5-1 part of co-carried exosome, 10-15 parts of lithospermum, 5-10 parts of astragalus root, 3-7 parts of rheum officinale, 3-5 parts of radix angelicae, 3-5 parts of angelica sinensis, 5-10 parts of pseudo-ginseng, 10-15 parts of chitosan and 20-25 parts of beta-sodium glycerophosphate. The invention greatly improves the transdermal absorption of the medicine, realizes intelligent administration, reduces side effects, has better effect, reduces the pain of patients, is suitable for healing various burn and scald wound surfaces and has wide application prospect.

Inventors

  • ZHANG WEIQIANG
  • WANG HUIJUAN
  • GAO YAWEI

Assignees

  • 养系列(山东)生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260328

Claims (10)

  1. 1. A pharmaceutical composition for treating burns and scalds in intensive care is characterized by comprising, by weight, 1-1.5 parts of analgesic temperature response liposome, 0.5-1 part of co-carried exosome, 10-15 parts of lithospermum, 5-10 parts of astragalus membranaceus, 3-7 parts of rheum officinale, 3-5 parts of radix angelicae, 3-5 parts of angelica sinensis, 5-10 parts of pseudo-ginseng, 10-15 parts of chitosan and 20-25 parts of beta-sodium glycerophosphate.
  2. 2. The pharmaceutical composition for treating burns and scalds in intensive care according to claim 1, wherein the preparation of the analgesic temperature responsive liposomes: S1, dissolving ketamine and sufentanil in hot ethanol, cooling to room temperature, volatilizing in an open way to obtain eutectic, filtering, washing, drying and crushing to obtain the ketamine and sufentanil eutectic; S2, adding dipalmitoyl phosphatidylcholine, cholesterol, ketamine and sufentanil eutectic into diethyl ether, dripping into a buffer solution, stirring and mixing, heating under reduced pressure to remove diethyl ether, performing ultrasonic treatment, and filtering to obtain the analgesic temperature response liposome.
  3. 3. The pharmaceutical composition for treating burns and scalds in intensive care according to claim 2, wherein the mass ratio of ketamine to sufentanil in step S1 is 40-50:1, the temperature of the hot ethanol is 60-70 ℃, the mass ratio of dipalmitoyl phosphatidylcholine, cholesterol, ketamine and sufentanil in step S2 is 5-8:1-3:0.8-1.2, the buffer is PBS buffer with ph=7.4, the time of the ultrasound is 15-30min, and the filtration is carried out through organic membranes of 0.45 μm and 0.22 μm in sequence.
  4. 4. The pharmaceutical composition for treating burns and scalds in intensive care according to claim 1, wherein the preparation method of the co-carried exosomes is as follows: T1, centrifugally removing exosomes in fetal calf serum, preparing a culture medium for culturing deer antler stem cells, collecting supernatant after the cells grow fully, and collecting deer antler stem cell exosomes by gradient centrifugation; T2, dissolving salvianolic acid B in a buffer solution, adding the pilose antler stem cell exosomes, incubating, and dialyzing to obtain a first medicine-carrying exosomes; And T3, dissolving tanshinone IIA in DMSO, mixing with the first medicine-carrying exosome aqueous solution, placing under an electroporation instrument, performing electric shock, taking out, centrifuging, and freeze-drying to obtain the co-carrying exosome.
  5. 5. The pharmaceutical composition for treating burns and scalds according to claim 4, wherein the mass ratio of salvianolic acid B, tanshinone IIA and pilose antler stem cell exosomes is 1-2:0.5-1:15-20, the culture medium in step T1 is a DMEM complete culture medium containing 8-12% fetal bovine serum, 0.5-1.5% penicillin and 0.5-1.5% streptomycin by volume fraction, the algebra of pilose antler stem cells is 5-7 generations, the incubation temperature in step T2 is 36-38 ℃ for 1-3h, the buffer is PBS buffer with pH=7.4, the protein content of the aqueous solution of the first drug-carrying exosomes is 0.3-0.4mg/mL, the number of times of electric shock in step T3 is 3-7, and the voltage is 0.5-1V.
  6. 6. A method for preparing a pharmaceutical composition for treating burns and scalds in intensive care according to any one of claims 1 to 5, comprising the steps of: (1) Mixing radix Arnebiae, radix astragali, radix et rhizoma Rhei, radix Angelicae Dahuricae, radix Angelicae sinensis, and Notoginseng radix, pulverizing, sieving to obtain Chinese medicinal powder, adding the Chinese medicinal powder into ethyl acetate, heating and reflux extracting, filtering, adding emulsifier F68 and 1, 2-propylene glycol into the filtrate to form emulsion, dripping into sodium alginate solution, emulsifying, adding into petroleum ether to form multiple emulsion, dripping into calcium chloride solution, crosslinking, centrifuging, washing, and drying to obtain Chinese medicinal microsphere; (2) Dissolving chitosan in acid liquor to obtain chitosan solution, and obtaining solution A; (3) Dissolving beta-sodium glycerophosphate in sodium bicarbonate solution to obtain solution B; (4) And (3) dropwise adding the solution B into the solution A, adding the traditional Chinese medicine microspheres, the analgesic temperature response liposome and the co-carried exosomes, and uniformly stirring and mixing to prepare the pharmaceutical composition for treating burns and scalds in intensive care.
  7. 7. The method according to claim 6, wherein the heating reflux extraction time in the step (1) is 4-8 hours, the solid-to-liquid ratio of the Chinese medicine powder to ethyl acetate is 1:5-10g/mL, the concentration of the sodium alginate solution is 2-4wt%, the concentration of the calcium chloride solution is 1-3wt%, and the crosslinking time is 4-6 hours.
  8. 8. The method according to claim 6, wherein the acid solution in the step (2) is 1-3wt% hydrochloric acid or acetic acid solution, and the chitosan solution has a concentration of 3-5wt%.
  9. 9. The method according to claim 6, wherein the concentration of sodium beta-glycerophosphate in the solution B in the step (3) is 50-60wt%, and the concentration of sodium bicarbonate solution is 0.3-0.5mol/L.
  10. 10. The method according to claim 6, wherein the pharmaceutical composition for treating burns and scalds in the intensive care of step (4) is in a liquid state at room temperature and forms a gel state at 37 ℃ after being applied to the skin.

Description

Pharmaceutical composition for treating burns and scalds in intensive care and preparation method thereof Technical Field The invention relates to the technical field of medicines, in particular to a pharmaceutical composition for treating burns and scalds in intensive care and a preparation method thereof. Background The reasons for the burn and scald are different, and the principle of treating the burn and scald is consistent, and the main problems of wound pain, progressive necrosis, easy infection, scar healing and the like need to be solved. Along with the development of society, the theoretical level and clinical experience of traditional Chinese medicine for treating burns and scalds are improved to a certain extent, and certain progress is made in the field of the traditional Chinese medicine. In ancient Chinese medicine literature, burn and scald treatments are described, for example, the works of "fifty two diseases prescription", medical entrance, field fire sore ", great university of ulcer" and "ghost's prescription". In Qing dynasty Chen Shiduo, the "Dongtian Aoming" states that "treating fire-burning symptoms, both internal and external treatment are necessary, and fire toxin is easy to resolve. The principle of treating both internal and external burns is proposed. According to the experience of treating burns and scalds in ancient and modern medicine, the modern traditional Chinese medicine summarizes five treatment principles of clearing heat and detoxicating, nourishing yin and promoting fluid production, tonifying qi and regulating spleen, activating blood and removing blood stasis, and expelling pus in the middle-jiao. The treatment of burns and scalds is mainly divided into 2 kinds of dry therapy and wet therapy. The Western medicine mainly adopts dry therapy for treating burn, which mainly depends on interference of exogenous factors, antibiotics and operation modes, and firstly dries and crusts the wound surface, and then cuts the scab and skin grafts through operation. The wet therapy is widely applied to the process of treating burns and scalds by traditional Chinese medicine, the external application of the traditional Chinese medicine and the preparation thereof creates a pharmacological environment with proper humidity, and from the perspective of physiological regeneration and repair, necrotic tissues are liquefied, and recoverable tissues are preserved, so that the wound surface can be healed autonomously. However, the traditional Western medicine or traditional Chinese medicine treatment for burns and scalds has severe pain, slow curative effect and poor effect, and is easy to leave scars. Chinese patent (CN 101884709A) discloses an ointment for curing burn and scald, its raw materials are made up by using frankincense, myrrh, catechu, dragon's blood, carthamus flower, dragon's bone, beeswax, elephant skin, coptis root, rhubarb and sesame oil. The invention can promote blood circulation, remove blood stasis, astringe and relieve pain, thereby achieving the purpose of treating burns and scalds. Chinese patent (CN 107714781A) discloses a pharmaceutical composition for treating burns and scalds, which consists of two functional components a and b, wherein the component a has the effects of clearing heat, detoxicating, diminishing inflammation and relieving pain, the raw materials comprise 10-40 parts of giant knotweed rhizome, 5-35 parts of dandelion, 5-35 parts of lithospermum, 1-20 parts of borneol, the component b has the effects of promoting blood circulation, removing blood stasis, removing necrotic tissue and promoting granulation, the raw materials comprise 10-40 parts of rheum officinale, 10-40 parts of garden burnet root, 1-20 parts of radix codonopsis pilosulae, 1-20 parts of astragalus mongholicus and 50-200 parts of egg white. The traditional Chinese medicine composition can be used separately according to different effects of traditional Chinese medicines, can fully exert the synergistic effect of the traditional Chinese medicine components, has more remarkable and stable curative effect compared with single administration, is mainly used for treating the symptoms caused by early and middle stage sore surface redness, swelling, heat and pain and obvious exudation of burns and scalds, reduces wound infection, accelerates wound healing and effectively reduces the formation of ulcer phenomena at the positions of burns and scalds. However, the existing pure traditional Chinese medicine burn and scald medicine still has the problems of insufficient curative effect, long time, poor effect, easy scar leaving and the like. Disclosure of Invention The invention aims to provide a pharmaceutical composition for treating burns and scalds in intensive care and a preparation method thereof, which have the advantages of better promotion of wound healing, better reduction of scar generation, cooperative repair, great improvement of transdermal absorption of medicines, intelligent