CN-122005663-A - Application of aconite in treating kidney-yang deficiency type osteoporosis

Abstract

The invention discloses an application of aconite in treating kidney-yang deficiency type osteoporosis, relates to the technical field of traditional Chinese medicines, and relates to a aconite composition for treating kidney-yang deficiency type osteoporosis, which comprises aconite extract and a pharmaceutically acceptable carrier, wherein the aconite extract is prepared by water extraction or alcohol extraction, the aconite alkaloid content accounts for 1% -10% of the total weight of the extract, and the composition is prepared into an oral preparation or injection. The invention effectively solves the problems of single target point and limited curative effect of the traditional medicine through the synergistic effect of the anti-aging and osteogenesis promoting double mechanisms, the aconite can improve the kidney-yang deficiency state of mice caused by hydrocortisone injection, and the cAMP/cGMP ratio is normal, so that the pathological internal environment of the kidney-yang deficiency is twisted as a whole.

Inventors

• GAN YINGYING
• ZHOU YING
• ZHANG LU

Assignees

• 重庆医科大学

Dates

Publication Date

20260512

Application Date

20260130

Claims (10)

1. 1. The application of aconite in treating the kidney-yang deficiency type osteoporosis is characterized in that the aconite extract is extracted and applied to the preparation of medicaments for treating the kidney-yang deficiency type osteoporosis.
2. 2. A aconite composition for treating kidney-yang deficiency type osteoporosis, comprising aconite extract and a pharmaceutically acceptable carrier, wherein the aconite extract is prepared by water extraction or alcohol extraction, the aconite alkaloid content accounts for 1% -10% of the total weight of the extract, and the composition is configured into oral preparation or injection.
3. 3. The aconite composition according to claim 2, further comprising a dried ginger extract in a weight ratio of 1:0.5 to 1:2, wherein the dried ginger extract is used for reducing toxicity of the aconite and enhancing osteogenic effect by synergistic effect, and wherein the dried ginger extract is obtained by reflux extraction with ethanol, wherein gingerol content is not less than 3%, and the compatibility ratio is 1:1.
4. 4. The aconite composition of claim 3, further comprising an osteogenic promoter, said osteogenic promoter being one or more of calcium, vitamin D, or alendronate sodium, wherein the weight ratio of aconite extract to osteogenic promoter is 1:0.1 to 1:5.
5. 5. A method for preparing a aconite composition according to any one of claims 1-4, which comprises the steps of crushing aconite raw materials, extracting with water, adding water 6-10 times of the weight of the raw materials, mixing the extracting solutions, and concentrating to obtain fluid extract with a relative density of 1.10-1.15.
6. 6. The preparation method of claim 5, wherein the water extraction method further comprises an alcohol precipitation step, wherein ethanol is added into the concentrated fluid extract to enable the alcohol content to reach 60% -70%, the fluid extract is obtained after standing for 24 hours, and the aconite extract is obtained after recovering the ethanol.
7. 7. The use of aconite root according to claim 5 for treating osteoporosis due to kidney-yang deficiency, wherein the extraction temperature of the water extraction method is 80-100 ℃, the extraction time is 1-2 hours, and the extraction is 2-3 times.
8. 8. A quality control method of aconite composition according to any one of claims 1-4, wherein the quality control method comprises the following steps of determining aconite alkaloid content by HPLC method, determining aconitine, neoaconitine and aconitine content not less than 1.5%, checking microorganism limit, and determining heavy metal content not more than 10ppm.
9. 9. A pharmaceutical package comprising a aconite composition according to any one of claims 1-4 together with instructions describing the composition, efficacy, dosage and care of the drug.
10. 10. A system for diagnosing and selecting a medicament for treating osteoporosis due to kidney-yang deficiency, comprising a traditional Chinese medicine syndrome scoring module and a medicament proportion recommending module, wherein the optimal compatibility proportion and dosage of the aconite composition according to any one of claims 1-4 are automatically recommended according to the syndrome score of a patient.

Description

Application of aconite in treating kidney-yang deficiency type osteoporosis Technical Field The invention relates to the technical field of traditional Chinese medicines, in particular to an application of aconite in treating kidney-yang deficiency type osteoporosis. Background Osteoporosis (osteoporosis, OP) is a systemic degenerative bone disease characterized by reduced bone mass, damaged bone microstructure, increased bone fragility, and increased risk of fracture. Epidemiological investigation of osteoporosis of residents in China in 2018 shows that the incidence rate of osteoporosis of people over 65 years old reaches 32.0%, wherein the incidence rate of osteoporosis of men is 10.7%, the incidence rate of osteoporosis of women is 51.6%, the fracture incidence rate of osteoporosis increases with age, and the fracture rate of people over 80 years old can reach 37%. At present, the Western medicine for treating osteoporosis mainly uses hormone medicines and hormone replacement therapy, and has certain curative effect, but adverse reaction and high price. The traditional Chinese medicine is used as an anti-osteoporosis treatment means, and has the unique advantage of multi-component and multi-target cooperative treatment. In recent years, traditional Chinese medicine shows unique advantages in preventing and treating osteoporosis, and is attracting attention of more and more researchers. Radix Aconiti lateralis is as one of yang-warming herbs in traditional Chinese medicine, has effects of restoring yang, rescuing collapse, nourishing fire and supporting yang, and is commonly used for treating diseases related to yang deficiency and cold. Modern pharmacological researches have found that aconite contains various active ingredients such as aconite alkaloid, polysaccharide, volatile oil, etc., and has multiple biological activities of anti-inflammatory, antioxidant, immunoregulatory and bone metabolism promoting. In particular, in the research of osteoporosis, aconite root has good anti-osteoporosis potential, and the action mechanism of aconite root may be involved in the aspects of promoting osteoblast differentiation and bone formation, inhibiting osteoclast activity, improving bone marrow microenvironment and the like. According to the main symptoms and clinical manifestations of OP, the pathogenesis of the traditional Chinese medicine is mainly spleen-kidney deficiency of patients, bone marrow is biochemically inactive, bone is malnourished and weak, spleen-kidney yang deficiency syndrome is common, wherein kidney-yang deficiency type osteoporosis is large, and the traditional Chinese medicine for preventing and treating kidney-yang deficiency type osteoporosis is clinically commonly used. In the common classic formula for tonifying kidney yang, aconite root is taken as a monarch drug, or dried Jiang Gancao is added for detoxification, or yin nourishing drug is added, so that the effect of treating kidney yang deficiency OP is exerted. The aconite has no ginger and no heat, and the dried ginger and the aconite have synergistic effect and can effectively reduce the toxicity of the aconite. Furthermore, aging is considered to be one of the important risk factors for the development and progression of osteoporosis. With age, the level of oxidative stress of the body increases, cell aging accelerates, and bone marrow mesenchymal stem cells (BMSCs) have reduced differentiation ability in the osteogenic direction, resulting in reduced bone formation ability. Research shows that aconite can improve the state of the aged organism by means of scavenging free radicals, enhancing the activity of antioxidant enzymes, delaying cell aging and the like, thereby improving the osteogenic function. Some animal experiments and cell experiments also prove that the aconite or the extract thereof can obviously promote bone density and improve trabecular bone structure in an aging-related model, and indicate that the aconite or the extract thereof has an anti-aging mechanism for resisting osteoporosis. In conclusion, the aconite root, serving as a traditional Chinese medicine with the effects of warming yang, tonifying kidney, resisting aging and promoting bone metabolism, has good application prospect in preventing and treating osteoporosis. The specific mechanism of the aconite for treating the osteoporosis through the anti-aging and osteogenesis is studied in depth, so that scientific basis is hopeful for clinical application of the aconite, and novel theoretical support and treatment thought are provided for the traditional Chinese medicine intervention of the osteoporosis. Disclosure of Invention The invention aims to solve the defects existing in the prior art, and provides an application of aconite in treating kidney-yang deficiency type osteoporosis. In order to achieve the above purpose, the present invention adopts the following technical scheme: The application of aconite in treating the kidney-yang deficiency typ