Search

CN-122005679-A - Oral ulcer patch containing banana starch Janus particles and preparation method of oral ulcer patch

CN122005679ACN 122005679 ACN122005679 ACN 122005679ACN-122005679-A

Abstract

The invention belongs to the field of oral patches, and particularly relates to an oral ulcer patch of banana starch Janus particles and a preparation method thereof. According to the invention, natural starch extracted from bananas is taken as a main raw material, the Pickering emulsion can be prepared by modifying and regulating the hydrophobicity of the natural starch, and extracts prepared from dandelion, dried orange peel and litchi peel and lotus leaf extracts are added, so that the finally prepared banana starch Janus particles can remarkably inhibit the growth of candida albicans and escherichia coli causing canker sores, and have remarkable treatment effects on canker sores. The banana starch has the advantages of reproducibility, biocompatibility and degradability, is suitable for green application, has low cost, has no side effect, does not generate anaphylactic reaction, is obviously improved in safety when being used for treating canker sore, and can be used as a stock medicine when canker sore repeatedly attacks.

Inventors

  • XIAO DAO
  • SHENG ZHANWU
  • YANG YANG
  • ZHENG LILI
  • AI BINLING
  • ZHENG XIAOYAN
  • LIU YONGXIA

Assignees

  • 中国热带农业科学院热带生物技术研究所

Dates

Publication Date
20260512
Application Date
20260224

Claims (10)

  1. 1. The oral ulcer composition of banana starch Janus granules is characterized by comprising, by weight, 10-20 parts of banana starch, 5-10 parts of lotus leaf extract, 15-25 parts of dandelion, 10-15 parts of dried orange peel, 13-17 parts of litchi peel, 1-4 parts of octenyl succinic anhydride and 30-100 parts of long-chain fatty acid.
  2. 2. The oral ulcer composition of banana starch Janus granules according to claim 1, wherein the oral ulcer composition comprises, by weight, 15 parts of banana starch, 7 parts of lotus leaf extract, 20 parts of dandelion, 12 parts of dried orange peel, 15 parts of litchi peel, 3 parts of octenyl succinic anhydride and 50 parts of long-chain fatty acid.
  3. 3. A banana starch Janus particle oral ulcer composition as claimed in claim 1 wherein the long chain fatty acid is lauric acid or myristic acid.
  4. 4. A banana starch Janus granule canker sore composition as claimed in claim 1, wherein the banana starch preparation method comprises the following steps: cleaning green bananas, slicing, immersing in a citric acid solution with the mass concentration of 0.3-0.5% for 3-5min, pulping with the citric acid solution, adding pectase and cellulase into the pulp for enzymolysis at 35-40 ℃ for 1-2h, filtering to remove precipitate, adjusting the pH value to 4-6, centrifuging, taking the lower layer liquid, adding water for centrifuging again, taking the lower layer liquid, and performing vacuum freeze drying to obtain banana starch; The preparation method of the lotus leaf extract comprises the following steps: Fresh lotus leaves are taken, water with the same weight as the lotus leaves is added for pulping after cleaning, the filtrate is filtered, water with the weight of 1/2 of the weight of the lotus leaves is continuously added into filter residues for filtering, the filtrate is combined, absolute ethyl alcohol with the same volume as the filtrate is added for fully stirring, standing is carried out for 24 hours at the temperature of 4 ℃, and the supernatant is taken for freeze drying, thus obtaining the lotus leaf extract.
  5. 5. A method for preparing a canker sore composition of banana starch Janus particles as claimed in claim 1 or 2, comprising the steps of: (1) Decocting herba Taraxaci and pericarpium Citri Tangerinae in water for 20-30min, filtering to obtain filtrate, and drying to obtain extract I, pulverizing litchi pericarp, soaking in 95% ethanol at 40-50deg.C, and removing ethanol to obtain extract II; (2) Stirring and mixing banana starch and deionized water to obtain starch emulsion, adding octenyl succinic anhydride into the starch emulsion, adding sodium hydroxide to adjust the pH to 7-9, stirring for reaction, filtering after the reaction is finished, taking precipitate, cleaning, drying, and crushing a dried product to obtain hydrophobically modified starch particles; (3) Adding the hydrophobic modified starch particles and the long-chain fatty acid into water, uniformly mixing the hydrophobic modified starch particles and the long-chain fatty acid, homogenizing the mixture to form emulsion, refrigerating the homogenized emulsion, taking out the emulsion, and standing the emulsion at room temperature to stabilize the emulsion to obtain Pickering emulsion; (4) Dissolving the first extract, the second extract and the lotus leaf extract in an acetic acid aqueous solution to form a mixed solution with the mass concentration of 1mg/mL, regulating the pH value to 4.5-5, continuously stirring for 3-5h, removing residues of insoluble particles by a filter membrane with the pore diameter of 0.45 mu m, preheating for 15min at 50 ℃, adding a sodium Tripolyphosphate (TPP) aqueous solution with the mass concentration of 0.5mg/mL under the stirring condition at the speed of 700rpm, and reacting for 20-30min to obtain a mixed dispersion; (5) Adding the mixed dispersion liquid into the Pickering emulsion obtained in the step (4) according to the volume ratio of 1:1 to react for 20min to obtain a mixed system of the Pickering emulsion and the Pickering emulsion, removing redundant chitosan nanoparticle dispersion liquid, adding deionized water to sequentially carry out ultrasonic treatment and centrifugal treatment, removing supernatant, and repeating centrifugation for 2-3 times to obtain the composition.
  6. 6. The preparation method of claim 5, wherein in the step (1), dandelion and dried orange peel are added with water 5-8 times of the total weight and decocted for 20-30min, and ethanol with the volume concentration of 95% is 5-6 times of the weight of litchi peel and soaked for 30-60min; In the step (2), adding deionized water into banana starch to obtain starch emulsion with the mass concentration of 20%, adding octenyl succinic anhydride with the mass concentration of 0.3% of banana starch into the starch emulsion, stirring for 0.5-1 hour, reacting for 2.5-3 hours in a water bath at 30-45 ℃, repeatedly washing the precipitate with deionized water for 3-4 times, and drying at 40-50 ℃; In the step (3), the homogenization condition is 12000rpm for 2-4min, cold storage at 1-4 ℃ for 30-50min, and room temperature control at 20-30 ℃; in the step (4), the volume ratio of the sodium Tripolyphosphate (TPP) aqueous solution to the mixed solution is 1:5-7; in the step (5), the ultrasonic treatment is carried out at 40 ℃ for 30min and the centrifugal treatment is carried out at 4000rpm for 5min.
  7. 7. Use of a composition according to any one of claims 1 to 4 for the preparation of a formulation for the treatment of canker sores, inhibition of candida albicans and/or inhibition of escherichia coli.
  8. 8. The use according to claim 7, wherein the formulation is an external formulation.
  9. 9. The use according to claim 8, wherein the external preparation is a patch, a paste, an ointment, a gel, a paste and/or a liniment.
  10. 10. A patch for treating canker sores, comprising the composition described above.

Description

Oral ulcer patch containing banana starch Janus particles and preparation method of oral ulcer patch Technical Field The invention belongs to the field of oral patches, and particularly relates to an oral ulcer patch of banana starch Janus particles and a preparation method thereof. Background Canker sores are a common disease of the oral mucosa and are manifested as circular or oval painful ulcerations in the oral lips, palate, tongue and cheeks, etc. The etiology of the dental ulcer is complex, the burning sensation is obvious during the attack, the daily diet and speaking of people are seriously influenced, and the dental ulcer is easy to recur, so that the development of the medicine for treating the dental ulcer has important significance for the daily life of people. The oral patch has simple application method, can continuously exert the drug effect, and has remarkable treatment effect on canker sore. However, the existing oral patches are often prepared from a single component, are extremely easy to produce side effects after long-term use, and have great limitation in the treatment of canker sores, in particular to the treatment of recurrent canker sores. Therefore, development of the canker sore patch which is green and safe, has obvious curative effect and low toxic and side effects and is suitable for long-term use is imperative. Disclosure of Invention The invention provides a banana starch Janus granule dental ulcer patch and a preparation method thereof, wherein banana starch is added into the dental ulcer patch, and the prepared Janus granule not only can remarkably improve the treatment effect on dental ulcer, but also can remarkably inhibit the recurrence of dental ulcer, and is suitable for long-term use. The technical scheme of the invention is realized as follows: The composition for treating canker sore of banana starch Janus granules comprises, by weight, 10-20 parts of banana starch, 5-10 parts of lotus leaf extract, 15-25 parts of dandelion, 10-15 parts of dried orange peel, 13-17 parts of litchi peel, 1-4 parts of octenyl succinic anhydride and 30-100 parts of long-chain fatty acid. Further, the novel banana oil-based food comprises, by weight, 15 parts of banana starch, 7 parts of lotus leaf extract, 20 parts of dandelion, 12 parts of dried orange peel, 15 parts of litchi peel, 3 parts of octenyl succinic anhydride and 50 parts of long-chain fatty acid. Further, the long-chain fatty acid is lauric acid or myristic acid; further, the preparation method of the banana starch comprises the following steps: Cleaning green banana (ripeness is 6-7 ripeness, skin is completely green, pulp hardness is high, softening is not started), slicing, immersing in citric acid solution with mass concentration of 0.3-0.5% for 3-5min, pulping with the citric acid solution, adding pectase and cellulase into the pulp for enzymolysis at 35-40 ℃ for 1-2h, filtering to remove precipitate, adjusting pH to 4-6, centrifuging, taking the supernatant, centrifuging again with water, taking the supernatant, and vacuum freeze-drying to obtain banana starch; Further, the preparation method of the lotus leaf extract comprises the following steps: Fresh lotus leaves are taken, water with the same weight as the lotus leaves is added for pulping after cleaning, the filtrate is filtered, water with the weight of 1/2 of the weight of the lotus leaves is continuously added into filter residues for filtering, the filtrate is combined, absolute ethyl alcohol with the same volume as the filtrate is added for fully stirring, standing is carried out for 24 hours at the temperature of 4 ℃, and the supernatant is taken for freeze drying, thus obtaining the lotus leaf extract. A composition of banana starch Janus particles for oral ulcer, the preparation method comprising the following steps: (1) Decocting herba Taraxaci and pericarpium Citri Tangerinae in water for 20-30min, filtering to obtain filtrate, and drying to obtain extract I, pulverizing litchi pericarp, soaking in 95% ethanol at 40-50deg.C, and removing ethanol to obtain extract II; (2) Stirring and mixing banana starch and deionized water to obtain starch emulsion, adding octenyl succinic anhydride into the starch emulsion, adding sodium hydroxide to adjust the pH to 7-9, stirring and reacting for 3-5h, filtering after the reaction is finished, taking and cleaning precipitate, drying, and crushing a dried product to obtain hydrophobically modified starch particles; (3) Adding the hydrophobic modified starch particles and the long-chain fatty acid into water, uniformly mixing the hydrophobic modified starch particles and the long-chain fatty acid, homogenizing the mixture to form emulsion, refrigerating the homogenized emulsion, taking out the emulsion, and standing the emulsion at room temperature to stabilize the emulsion to obtain Pickering emulsion; (4) Dissolving the first extract, the second extract and the lotus leaf extract in an acetic acid aqueous solution t