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CN-122005687-A - Extraction and purification method and application of Russian tea polyphenol

CN122005687ACN 122005687 ACN122005687 ACN 122005687ACN-122005687-A

Abstract

The invention discloses an extraction and purification method and application of Russian tea polyphenol, and belongs to the field of separation and application of plant natural products. The invention comprises the steps of mixing Russian dry tea powder with ethanol according to a set feed liquid ratio, carrying out ultrasonic treatment on the mixed liquid to obtain a Russian tea polyphenol crude extract, adding the Russian tea polyphenol crude extract into a chromatographic column filled with macroporous resin, eluting and adsorbing by using an ethanol solution after adsorption balance is achieved, recovering the eluting liquid, and freeze-drying to obtain the purified Russian tea polyphenol extract. The Russian tea polyphenol extract obtained by the invention has strong free radical scavenging capability, can effectively inhibit active oxygen of HepG2 cells, and can be used for research and development of antioxidant functional foods and medicines.

Inventors

  • WEI RAN
  • WANG YUHANG
  • SU ZHUCHENG
  • WANG YANFEI
  • Zhai Shengfan
  • SONG YIWEI

Assignees

  • 浙江农林大学

Dates

Publication Date
20260512
Application Date
20260204

Claims (10)

  1. 1. The method for extracting and purifying Russian tea polyphenol is characterized by comprising the following steps of: 1) Pulverizing dried Russian tea, sieving to obtain Russian dry tea powder, mixing Russian dry tea powder with ethanol according to a set feed liquid ratio, performing ultrasonic treatment on the mixed liquid, and filtering after ultrasonic treatment to obtain Russian tea polyphenol crude extract; 2) Adding the crude extract of Russian tea polyphenol obtained in the step 1) into a chromatographic column filled with macroporous resin, eluting with ethanol solution after reaching adsorption equilibrium, recovering the eluting liquid, and freeze-drying to obtain the purified Russian tea polyphenol extract.
  2. 2. The method according to claim 1, wherein in the step 1), the volume percentage concentration of the ethanol in the step 1) is 40-80%, and the ratio of the Russian dry tea powder to the ethanol is 1:20-1:100 g/mL.
  3. 3. The method according to claim 1, wherein in step 1), the ultrasound temperature is 20-60 ℃ and the ultrasound time is 10-30 min.
  4. 4. The method according to claim 1, wherein in the step 1), the volume fraction of ethanol is 40-60%, the feed liquid ratio is 1:20-1:60 g/mL, the ultrasonic temperature is 30-50 ℃, and the ultrasonic time is 10-20 min.
  5. 5. The method according to claim 1, wherein in the step 1), the volume fraction of ethanol is 50%, the feed liquid ratio is 1:43.5 g/mL, the ultrasonic temperature is 43 ℃, and the ultrasonic time is 14 min.
  6. 6. The method of claim 1, wherein in step 2), the macroporous resin is a D101 resin.
  7. 7. The method according to claim 1, wherein in step 2), the volume fraction of the ethanol solution used for elution adsorption is 70%.
  8. 8. An extract of russian tea polyphenols prepared by the process according to any one of claims 1-7.
  9. 9. Use of the russian tea polyphenol extract of claim 8 in a product for inhibiting HepG2 cell ROS levels in vitro.
  10. 10. Use of the russian tea polyphenol extract of claim 8 for preparing an antioxidant functional food or medicament.

Description

Extraction and purification method and application of Russian tea polyphenol Technical Field The invention belongs to the field of separation and application of natural plant products, and particularly relates to an extraction and purification method and application of Russian tea polyphenol. Background Russian tea (Malus toringoides (red.) Hughes) belongs to Malus genus of Rosaceae, is a perennial shrub or small arbor, and has ecological value and medicinal potential. In recent years, along with the rising research of natural products, russian tea is gradually becoming a research hotspot in the fields of food science, pharmacy and functional food development because leaves and fruits of Russian tea are rich in bioactive components such as polyphenol, amino acid, trace elements and the like. Plant Polyphenols (Polyphenols) are an important class of secondary metabolites, the chemical structure of which is characterized by a benzene ring as a backbone and a plurality of hydroxyl groups. Such compounds exhibit a variety of nature, and over 8000 structurally different polyphenols have been identified. The plant polyphenol is widely distributed in natural diet foods such as vegetables, berry fruits, fermented tea drinks, grape brewed products, woody plant seeds and the like, has various biological activities and health benefits, and can be mainly divided into phenolic acids (such as chlorogenic acid and gallic acid), flavonoids (including flavonols, flavanones, isoflavone and the like), tannins (hydrolyzed tannins and condensed tannins) and the like. Wherein the flavonoid compound accounts for 60% of the total plant polyphenol. The existing research on Russian tea is very limited, and most of the research is focused on the aspect of functional activity research of crude extracts, and the lack of basic component identification, functional substance extraction and purification, process optimization and functional activity mechanism exploration restrict the conversion of the Russian tea from basic research to industrial application. There is no report about deep research on the aspects of extraction and purification process optimization and biological activity of Russian tea polyphenol. Disclosure of Invention The invention aims to overcome the defects of the prior art and provides an extraction and purification method and application of Russian tea polyphenol. In order to achieve the above object, the present invention provides a method for extracting and purifying russian tea polyphenol, comprising the steps of: 1) Pulverizing dried Russian tea, sieving to obtain Russian dry tea powder, mixing Russian dry tea powder with ethanol according to a set feed liquid ratio, performing ultrasonic treatment on the mixed liquid, and filtering after ultrasonic treatment to obtain Russian tea polyphenol crude extract; 2) Adding the crude extract of Russian tea polyphenol obtained in the step 1) into a chromatographic column filled with macroporous resin, eluting with ethanol solution after reaching adsorption equilibrium, recovering the eluting liquid, and freeze-drying to obtain the purified Russian tea polyphenol extract. According to a preferred embodiment of the invention, the concentration of ethanol in the step 1) is 40-80% by volume, and the ratio of Russian dry tea powder to ethanol is 1:20-1:100 g/mL. The ultrasonic temperature is 20-60 ℃ and the ultrasonic time is 10-30 min. Further, in the step 1), the volume fraction of the ethanol is 40-60%, the feed liquid ratio is 1:20-1:60 g/mL, the ultrasonic temperature is 30-50 ℃, and the ultrasonic time is 10-20 min. More preferably, the volume fraction of the ethanol is 50%, the feed liquid ratio is 1:43.5 g/mL, the ultrasonic temperature is 43 ℃, and the ultrasonic time is 14 min. According to a preferred embodiment of the invention, in step 2), the macroporous resin is a D101 resin. In step 2), the volume fraction of the ethanol solution used for eluting adsorption was 70%. The invention also provides the russian tea polyphenol extract prepared by the method. The invention relates to application of Russian tea polyphenol extract in preparing a product for inhibiting in-vitro HepG2 cell ROS level. Can be further used for preparing antioxidant functional foods or medicines, such as anti-aging products for improving the antioxidant capacity of tissues, organs and/or cells. Compared with the prior art, the invention has the following effects and advantages: 1) The invention analyzes main components and monomer components in dried Russian tea, and develops a Russian tea polyphenol extraction and purification method based on ultrasonic auxiliary extraction and macroporous resin purification, wherein ultrasonic extraction is applied to the extraction of Russian tea polyphenol, and an optimal extraction process is obtained. 2) The evaluation of the H 2O2 induced HepG2 oxidative stress model shows that the free radical scavenging ability of the purified polyphenol is obviously