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CN-122005729-A - Centipede toxin-vanquishing drink-treating psoriasis nano micelle transdermal preparation and preparation method thereof

CN122005729ACN 122005729 ACN122005729 ACN 122005729ACN-122005729-A

Abstract

The invention belongs to the technical field of modern traditional Chinese medicine preparations, and particularly discloses a nano micelle transdermal preparation for treating psoriasis by using centipede toxin-vanquishing drinks and a preparation method thereof, wherein the preparation comprises, by weight, 5-15 parts of an effective component extract of the centipede toxin-vanquishing drinks, 20-40 parts of a nano micelle carrier material, 2-8 parts of a transdermal absorption accelerator and 37-73 parts of a medicinal auxiliary material, and the centipede toxin-vanquishing drinks comprise 3g of centipede, 30g of lithospermum, 30g of glabrous greenbrier rhizome, 30g of winged euonymus twig, 20g of zaocys dhumnade and 10g of liquorice. The preparation is based on a classical centipede toxin-vanquishing drink prescription, combines a nano micelle technology, improves the transdermal penetration efficiency of the medicine and the targeting of a lesion part, enhances the treatment effect, reduces adverse reactions, has a stable and controllable preparation process, and is suitable for industrial production.

Inventors

  • An Yuepeng
  • YANG SUQING
  • REN YUKUN
  • ZHANG QING

Assignees

  • 黑龙江中医药大学

Dates

Publication Date
20260512
Application Date
20260331

Claims (10)

  1. 1. The nano micelle transdermal preparation for treating psoriasis by using the centipede toxin-vanquishing drink is characterized by comprising the following components, by weight, 5-15 parts of an effective component extract of the centipede toxin-vanquishing drink, 20-40 parts of a nano micelle carrier material, 2-8 parts of a transdermal absorption promoter and 37-73 parts of a pharmaceutic adjuvant; wherein the composition of the centipede toxin-vanquishing drink comprises 3g of centipede, 30g of lithospermum, 30g of glabrous greenbrier rhizome, 30g of winged euonymus twig, 20g of zaocys dhumnade and 10g of liquorice.
  2. 2. The centipede toxin-vanquishing drink for treating psoriasis, as claimed in claim 1, wherein the nano micelle carrier material is one or more of polyethylene glycol-polylactic acid-glycolic acid copolymer, hyaluronic acid-dioleoyl phosphatidyl ethanolamine and polyethylene glycol-polycaprolactone, the particle size of the nano micelle is 100-200 nm, zeta potential is-25 to-15 mV, and the encapsulation rate is more than or equal to 85%.
  3. 3. The nano-micelle transdermal preparation for treating psoriasis by using the centipede toxin-vanquishing beverage according to claim 1, wherein the transdermal absorption promoter is one or a combination of more of menthol, azone and limonene; The pharmaceutical auxiliary materials comprise a cosolvent, an antioxidant and a preservative, wherein the cosolvent is glycerin, the addition amount of the cosolvent is 5-10% of the total amount of the pharmaceutical auxiliary materials, the antioxidant is vitamin E, the addition amount of the antioxidant is 0.1-0.5% of the total amount of the pharmaceutical auxiliary materials, and the preservative is benzalkonium bromide, and the addition amount of the preservative is 0.01-0.05% of the total amount of the pharmaceutical auxiliary materials.
  4. 4. A method for preparing a nano-micelle transdermal preparation for treating psoriasis by using a centipede toxin-vanquishing drink according to any one of claims 1 to 3, which comprises the following steps: s1, preparing an extract of an active ingredient of the centipede toxin-vanquishing drink; S2, preparing a nano micelle carrier material; and S3, preparing the centipede toxin-vanquishing drink-treating psoriasis nano micelle transdermal preparation.
  5. 5. The method for preparing the nano micelle transdermal preparation for treating psoriasis by using the centipede toxin-vanquishing drink according to claim 4, wherein the step S1 is specifically as follows: Step S11, weighing the medicinal materials according to the formula of the centipede toxin-vanquishing drink, crushing centipede and black-tip, sieving with a 30-40 mesh sieve, and carrying out reflux extraction with 70-80% ethanol to obtain an extract A; step S12, decocting radix arnebiae seu lithospermi, rhizoma smilacis glabrae, ramulus euonymi and liquorice with water to obtain an extracting solution B; And S13, concentrating the mixed extract under reduced pressure until no alcohol smell exists, obtaining a concentrated solution, diluting the concentrated solution with distilled water until the concentration is 0.5g/mL, sequentially extracting with petroleum ether and ethyl acetate with equal volumes, standing for layering, collecting an ethyl acetate extract phase, concentrating under reduced pressure and drying under the conditions of 40-50 ℃ and 0.06-0.08 MPa, and obtaining the centipede toxin-vanquishing drink active ingredient extract.
  6. 6. The preparation method of the centipede toxin-vanquishing drink for treating psoriasis nano micelle transdermal preparation according to claim 5, which is characterized in that in the step S11, the ratio of feed liquid in reflux extraction is 1 (10-20), the temperature of reflux extraction is 60-70 ℃, the time of reflux extraction is 2-3 h, and the reflux extraction is 2-3 times; In the step S12, the ratio of the materials to the liquids is 1 (8-15), the temperature of the decoction and the extraction is 90-100 ℃, the time of the decoction and the extraction is 1.5-2.5 h, and the decoction and the extraction are carried out for 2-3 times.
  7. 7. The method for preparing the nano micelle transdermal preparation for treating psoriasis by using the centipede toxin-vanquishing drink according to claim 4, wherein the step S2 is specifically as follows: S21, dissolving a nano micelle carrier material in an organic solvent, and stirring and dissolving to form an organic phase; The dosage ratio of the nano micelle carrier material to the organic solvent is 1g to 2mL, wherein the organic solvent is one or a combination of more of dichloromethane, acetone and ethanol; s22, dripping the organic phase into the water phase according to the volume ratio of the organic phase to the water phase of 1 (5-15), and stirring at the temperature of 30-40 ℃ until the organic solvent is completely volatilized; And S23, performing ultrasonic treatment and filtration by a microporous filter membrane to obtain the nano micelle carrier material.
  8. 8. The method for preparing the nano-micelle transdermal preparation for treating psoriasis by using the centipede toxin-vanquishing beverage according to claim 7, wherein in the step S23, the power of ultrasonic treatment is 100-300W, the time of ultrasonic treatment is 5-15 min, the temperature of ultrasonic treatment is 20-30 ℃, and the pore diameter of a microporous filter membrane is 0.22 μm.
  9. 9. The method for preparing the nano micelle transdermal preparation for treating psoriasis by using the centipede toxin-vanquishing drink according to claim 4, wherein the step S3 is specifically as follows: S31, weighing the components of the centipede toxin-vanquishing drink for treating psoriasis nano micelle transdermal preparation according to the weight parts, adding a cosolvent into the extract of the effective components of the centipede toxin-vanquishing drink, and stirring and dissolving; step S32, sequentially adding the nano micelle carrier material, the transdermal absorption accelerator, the antioxidant and the preservative, and uniformly stirring; And step S33, emulsifying, homogenizing and sterilizing to obtain the centipede toxin-vanquishing drink-treating psoriasis nano micelle transdermal preparation.
  10. 10. The preparation method of the centipede toxin-vanquishing drink for treating psoriasis nano micelle transdermal preparation according to claim 9, which is characterized in that in the step S33, the emulsifying speed is 10000-15000 r/min, the emulsifying time is 10-20 min, the homogenizing pressure is 20-40 MPa, and the homogenizing times are 2-3 times; The sterilization mode is moist heat sterilization, the sterilization temperature is 121 ℃, and the sterilization time is 15-20 min.

Description

Centipede toxin-vanquishing drink-treating psoriasis nano micelle transdermal preparation and preparation method thereof Technical Field The invention belongs to the technical field of traditional Chinese medicine modern preparations, and in particular relates to a centipede toxin-vanquishing drink-treating psoriasis nano micelle transdermal preparation and a preparation method thereof. Background Psoriasis is a common chronic inflammatory skin disease, has the characteristics of long disease course, easy recurrence and difficult cure, has complex pathogenesis and is currently considered to be related to various factors such as heredity, immunity, environment and the like. Clinically, psoriasis mainly presents with symptoms of erythema, scales, pruritus and the like, and seriously affects physical and mental health and life quality of patients. At present, the psoriasis treatment medicine mainly comprises western medicines such as external glucocorticoid, vitamin D 3 derivative, immunosuppressant and the like, and traditional Chinese medicine decoction, pill, ointment and the like. However, the existing therapeutic drugs have the defects that the external western medicines have quicker effect, but the adverse reactions such as skin atrophy, telangiectasis and drug resistance are easy to generate after long-term use, and the western medicines used for systemic administration possibly damage liver and kidney functions. The centipede toxin-vanquishing decoction is a classical prescription for clinically treating psoriasis, has the effects of clearing heat and detoxicating, promoting blood circulation by removing blood stasis, dispelling wind and relieving itching, and has the effects of eliminating toxin and resolving masses, activating collaterals and relieving pain for centipede, cooling blood and promoting blood circulation, detoxifying and promoting eruption for lithospermum, detoxifying and dehumidifying rhizoma smilacis glabrae, and the euonymus alatus for blood breaking and channel dredging, detoxifying and detumescence, the Zaocys for dispelling wind and activating collaterals, and the liquorice for harmonizing the medicines, and the medicines are combined to achieve the curative effect. However, the traditional centipede toxin-vanquishing decoction is mainly orally administered in a decoction form, and has the problems that firstly, after oral administration, the medicament needs to be absorbed through gastrointestinal tracts, the bioavailability is low, and the gastrointestinal tracts are possibly stimulated, secondly, the medicament is widely distributed in the body, is difficult to concentrate in a targeting manner at a skin lesion part, the treatment effect is limited, thirdly, the decoction is complicated to prepare, the carrying and the use are inconvenient, and the dosage is difficult to accurately control. In order to overcome the defects of oral preparations, researchers try to prepare traditional Chinese medicines into transdermal preparations, so that the medicines directly act on skin lesion sites, the local medicine concentration is improved, and the systemic adverse reaction is reduced. However, the traditional Chinese medicine transdermal preparation (such as ointment and cream) has the problem of low transdermal drug permeation efficiency, and most of the effective components of the traditional Chinese medicine are difficult to penetrate the skin to enter the dermis layer to play a role due to the barrier effect of the skin stratum corneum, so that the traditional Chinese medicine has slow effect and long treatment period. The nano micelle is a novel drug carrier, is formed by self-assembly of amphiphilic polymer materials in aqueous solution, and has the characteristics of small particle size, good water solubility, capability of wrapping lipophilic or hydrophilic drugs and the like. The drug is prepared into a nano micelle preparation, so that the solubility and stability of the drug can be obviously improved, and meanwhile, the nano micelle can pass through the hair follicle, sweat gland and other channels of the skin or through the permeation effect of the stratum corneum, so that the transdermal efficiency of the drug is improved, and the targeting of the drug at a lesion part is enhanced. In the prior art, although some traditional Chinese medicine nanometer transdermal preparations are studied, the problems of improper selection of carrier materials, low medicine encapsulation efficiency, poor transdermal effect, complex preparation process and the like exist, and the optimal design is not carried out on the specific prescription of the centipede toxin-vanquishing drink. Therefore, the centipede toxin-vanquishing drink for treating psoriasis nano micelle transdermal preparation and the preparation method thereof are to be developed in the field, and the problems can be effectively solved. Disclosure of Invention The invention aims to provide a centipede toxin-vanquishing drink-t