CN-122005748-A - Composition containing agilawood extract and preparation method and application thereof

Abstract

The invention discloses a composition containing agilawood extract, a preparation method and application thereof, and belongs to the technical field of biology, wherein the composition comprises 5-20 parts of agilawood extract, 20-60 parts of functional peptide and 5-25 parts of seaweed functional component, wherein the seaweed functional component is used for forming an embedding, gel or slow-release structure, the functional peptide is preferably chlorella pyrenoidosa peptide, which is prepared by freeze thawing ultrasonic assisted enzymatic hydrolysis and cation exchange chromatography, the agilawood extract is preferably agilawood chromone enriched extract, during preparation, the seaweed functional component is dissolved in water, dispersed or emulsified by adding the agilawood extract, and then the functional peptide is added for uniform mixing, and the composition can effectively improve the stability and bioavailability of active components and has good application prospect in preparing products for assisting in protecting acute, alcoholic, medicinal or fatty liver injury.

Inventors

• XU LIANFU
• CHEN YUMEI
• LI JUN
• XU ZIHAO

Assignees

• 北京奕德堂科技有限公司

Dates

Publication Date

20260512

Application Date

20260408

Claims (10)

1. 1. The composition containing the agilawood extract is characterized by comprising the following components: 5-20 parts of agilawood extract; 20-60 parts of functional peptide; 5-25 parts of seaweed functional components; wherein the seaweed functional component is used for forming an embedding, gel or slow release structure.
2. 2. The composition containing agalloch eaglewood extract according to claim 1, wherein said functional peptide is derived from chlorella pyrenoidosa peptide which is an active peptide component obtained by pulverizing chlorella pyrenoidosa, mixing with water, freeze thawing, performing ultrasonic treatment to obtain homogenate, performing enzymolysis with alkaline protease and flavourzyme, adsorbing the enzymolysis product with activated carbon, and separating by SP Sephadex C25 cation exchange chromatography.
3. 3. The composition of claim 1, wherein the agalloch eaglewood extract is an agalloch eaglewood chromone enriched extract.
4. 4. The composition of claim 1, wherein the agilawood extract is obtained by pulverizing agilawood raw materials, performing reflux extraction or ultrasonic extraction with 50% -95% ethanol, concentrating the extract, performing adsorption separation with macroporous adsorption resin, eluting with ethanol solution, and collecting the eluted part.
5. 5. The composition of claim 1, wherein the seaweed functional component is selected from at least one of sodium alginate, seaweed polysaccharide and seaweed polyphenol.
6. 6. A method for preparing a composition containing an extract of agilawood according to any of claims 1-5, comprising the steps of: s1, dissolving seaweed functional components in water to form a seaweed phase; S2, dispersing or emulsifying the agilawood extract in the seaweed phase to form a first mixed system; S3, adding the functional peptide into the first mixed system and uniformly mixing; s4, freeze-drying, spray-drying and granulating the mixed system to obtain the composition containing the agilawood extract.
7. 7. The method for preparing a composition containing an agalloch eaglewood extract according to claim 6, wherein the particle size of the granulated particles is 50-500 μm.
8. 8. Use of a composition comprising an agalloch eaglewood extract for the preparation of a product for the assisted protection of acute liver injury, alcoholic liver injury, drug-induced liver injury or fatty liver injury.
9. 9. An article of manufacture for aiding in the protection of liver damage comprising a pharmacologically effective amount of the composition comprising agalloch eaglewood extract of any one of claims 1 to 5.
10. 10. The article of claim 9, wherein the article is one of a solid beverage, a tablet, a capsule, a granule, an oral liquid, a gel, or a functional food.

Description

Composition containing agilawood extract and preparation method and application thereof Technical Field The invention belongs to the technical field of biology, and relates to a composition containing agilawood extract, and a preparation method and application thereof. Background Liver injury, especially alcoholic liver injury, fatty liver disease and drug-induced liver injury, is usually accompanied by pathological processes such as increased oxidative stress, activation of inflammatory response, lipid metabolism disorder and damage to hepatic cell membrane structure. The existing protection thought is mainly focused on a single antioxidant component, a single peptide active substance or a single carrier system, and can improve liver injury indexes to a certain extent, but the problems of insufficient dispersion stability of the active component, obvious gastrointestinal degradation, difficult cooperative delivery of fat-soluble and water-soluble components, limited multi-target regulation capability and the like generally exist. The prior patent discloses a composition for preventing and treating alcoholic liver injury, which consists of seaweed polyphenol, sodium alginate, abalone peptide and spermidine, wherein the seaweed polyphenol composite powder is formed by the combined action of the sodium alginate and the spermidine, and then the seaweed polyphenol composite powder is compounded with the abalone peptide, so that the stability is improved and the liver protection effect is improved. The proposal shows that the seaweed functional component can be used as an active source and a structuring carrier for constructing a stable composite system, and can be suitable for developing compositions related to liver injury. Another patent discloses Cordyceps militaris peptide, a compound, a preparation method thereof and application thereof in treating alcoholic liver injury diseases. The scheme obtains the active peptide with the functions of antioxidation and immunoregulation through enzymolysis and purification, and further adopts galactosylated bacterial cellulose as a delivery carrier to encapsulate the peptide and astaxanthin together, thereby realizing protection, absorption extension and liver targeting delivery of enzymolysis damage in gastrointestinal environment. The technology shows that if the peptide active substance is compounded with a proper functional carrier and a fat-soluble active substance, the in vivo stability, tissue distribution and liver delivery efficiency can be obviously improved. After the chlorella pyrenoidosa is crushed, freeze-thawing and ultrasonic homogenizing, alkaline protease and flavourzyme double enzymolysis, activated carbon adsorption and SP Sephadex C25 cation exchange chromatography, specific active peptide components can be obtained, and the effect of obviously relieving alcoholic liver injury is verified by a HepG2 cell model. This suggests that the chlorella-derived peptides have a clear functional basis in the liver protection direction, but still belong to a single active module that is slightly water-soluble in nature, lacking a synergistic delivery and structure stabilization design for lipid-soluble actives. The agilawood and the extract thereof, especially the part rich in 2- (2-phenethyl) chromone compounds, have better modern pharmacological development value. In the prior art, the study of acute liver injury of lignum Aquilariae Resinatum adopts evidence chains combining network pharmacology, molecular docking and cell experiment, which suggests that the lignum Aquilariae Resinatum active substance can participate in the processes of oxidation resistance, inflammation resistance and liver cell protection in a multi-target mode. Thus, the agilawood extract is suitable for being used as a fat-soluble main active component in a compound system, but has the problems of insufficient dispersibility and stability in a conventional dosage form, and needs to be improved by a proper carrier system. In summary, although the prior art discloses "the combination of seaweed functional components and peptides for liver protection", "the carrier delivery system of peptides and fat-soluble active substances" and "the preparation and liver protection effects of the chlorella pyrenoidosa peptides", a compound scheme for organically integrating the fat-soluble multi-target activity of agilawood extracts, the water-soluble liver protection activity of the chlorella pyrenoidosa peptides and the embedding, gel and slow release effects of seaweed functional components has not been formed. Therefore, it is necessary to provide a new composition for assisting in protecting liver injury, so as to solve the problems of single activity, poor stability, low delivery efficiency and insufficient synergistic effect in the prior art. The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be t