CN-122005749-A - Method for regulating and controlling conception of non-pregnant and non-oestrus dairy cows

Abstract

The invention provides a method for regulating and inducing conception of non-pregnant, non-oestrus dairy cows, which is characterized by comprising the following steps of 1) detecting LH and FSH levels and carrying out stage by stage, 2) regulating and targeted induction on dairy cows with different LH and FSH stage levels, and 3) artificial insemination. Aiming at the high-difficulty propagation cases of 37 sky, no oestrus, lasting corpus luteum and uterus residue, the targeting treatment and accurate regulation are realized. By adopting a prostaglandin PGF2 a+oxytocin+gonadotrophin releasing hormone GnRH combined intramuscular injection scheme, the sustained corpus luteum is thoroughly dissolved, residual embryo tissues in the body are removed, the uterus is purified, the quality of the subsequent conception environment is remarkably improved, frequent ultrasonic monitoring is not needed, individuation and refined hormone regulation are realized, the conception rate is remarkably improved, the one-embryo double-calf rate is stably improved, and the economic benefit of cultivation is greatly improved.

Inventors

• JIN YIDA
• YIN BO

Assignees

• 昆明天沃生物科技有限公司

Dates

Publication Date

20260512

Application Date

20260228

Claims (1)

1. 1. The method for regulating and controlling the conception of the non-pregnant oestrus dairy cows is characterized by comprising the following steps of: 1) Detecting LH and FSH levels and staging, determining luteinizing hormone LH and follicle stimulating hormone FSH levels in the peripheral blood or milk sample of dairy cows which have no oestrus behavior and are confirmed to be nonpregnant by ultrasonic examination after 37 days of hybridization, wherein the levels are divided into the following four stages according to the development degree of follicles: 11 FSH >2, LH <2, a dominant follicular recruitment phase; 12 When LH >2, FSH <2, dominant follicular dominant phase stage; 13 When LH >2 and FSH >2, is the preovulatory phase of follicular maturation; 14 When LH <2 and FSH <2, are embryonic residues, dominant luteal function, follicular resting phase; 2) Regulating and targeting induction of cows with different LH and FSH stage levels: 21 For all cows with LH <2, FSH <2, and embryo residue, dominant luteal function and follicular resting stage, intramuscular injection of prostaglandin PGF2 a + oxytocin + gonadotrophin releasing hormone GnRH is implemented, complete dissolution of the corpus luteum is realized, progesterone sources are cut off, and residual embryos which depend on progesterone to maintain weak activity lose hormone support and are thoroughly inactivated, so that pathological factors which interfere with follicular development are eliminated, specifically as follows: 211 On day 0, the levels of LH and FSH were determined, and PGF2 a was intramuscular injected to each cow in an amount of 0.01 ml/kg body weight, while oxytocin was intramuscular injected to each cow in an amount of 0.09IU/kg body weight to dissolve the corpus luteum, promote uterine smooth muscle contraction, discharge dead embryos and residual tissues, and purify the intrauterine environment; 212 48 hours after the step 211) of intramuscular injection of the oxytocin, injecting GnRH into each cow according to the dosage of 0.035 mug/kg body weight so as to promote the development of new follicular waves, carrying out targeted induction of double follicular formation and carrying out the regulation of one embryo and double calves; 22 For all cows in the dominant follicular recruitment phase of FSH >2, LH <2, determining LH and FSH levels, intramuscular injecting GnRH to each cow at a dose of 0.035 μg/kg body weight, intramuscular injecting PGF 2a to each cow at a dose of 0.01 ml/kg body weight on days 4-7, intramuscular injecting GnRH to each cow at a dose of 0.035 μg/kg body weight 48 hours after PGF 2a injection, promoting new follicular wave development, targeted inducing double follicular formation, and performing regulation of one calf; 23 Determining LH and FSH levels of cows in dominant follicular domination stages of LH >2 and FSH <2, intramuscular injecting GnRH into each cow according to the amount of 0.035 mug/kg body weight, intramuscular injecting PGF 2a into each cow according to the amount of 0.01 ml/kg body weight in days 3-5, intramuscular injecting GnRH into each cow according to the second time of 0.035 mug/kg body weight 48 hours after PGF 2a injection, promoting new follicular wave development, targeted inducing double follicular formation, and regulating and controlling one calf and two calves; 24 For cows in the preovulatory period of follicular maturation of all LH >2 and FSH >2, measuring the level of LH and FSH on day 0, intramuscular injecting GnRH into each cow according to the weight of 0.035 mug/kg, retaining trace progesterone secreted by the degenerated corpus luteum, providing necessary endocrine environment for the final maturation and normal ovulation of oocytes, promoting new follicular development, targeting induction of double follicular formation, and implementing regulation of one-embryo double calves; 3) Artificial insemination Steps 212), 22), 23), 24) 16-18 hours after the last GnRH injection, regular timed insemination is performed to match insemination time exactly with peak ovulation.

Description

Method for regulating and controlling conception of non-pregnant and non-oestrus dairy cows Technical Field The invention belongs to the technical field of cow breeding, and particularly relates to a method for judging, lasting corpus luteum targeted dissolution, uterine environment purification, follicular development regulation and control and targeted induction of cow conception based on endogenous hormone level aiming at 37 days of non-pregnant cows without oestrus behaviors after hybridization. Background The milk cows are usually initially judged to be pregnant in clinic after 37 days of estrus expression after mating, but the milk cows confirmed to be non-pregnant through ultrasonic detection mostly continuously stimulate ovaries by residual tissues after early embryo death to form persistent corpus luteum, the persistent corpus luteum continuously secretes progesterone and strongly inhibits follicular development and estrus expression, therefore, the pasture is mostly treated by adopting a conventional 0789 synchronous estrus program, namely, gonadotropin releasing hormone GnRH is injected at a high dose of 100-200 mu g/head for a fixed time to force the follicular development of the milk cows, rapidly remove inhibition and restore estrus ovulation, and then the timing insemination is carried out to reduce the number of non-pregnant days. But 0789 has poor treatment effect on the synchronous oestrus, low conception rate, usually 20% -35%, and low reproduction efficiency. Moreover, the prior art 0789 is used for treating the synchronous estrus, so that the problems of ovulation disorder, ovarian cyst and the like caused by ovarian hyperstimulation are easily caused. That is, the prior art does not implement precise regulation and control and induction according to the development condition of individual follicles, and is difficult to safely and stably realize efficient conception, and is more difficult to realize the goal of one-embryo double calves. In the prior art, prostaglandin PGF 2a is only adopted to carry out luteal dissolving treatment, and the residual embryonic tissues in the uterus are not effectively cleared, so that the residual embryonic tissues continuously stimulate the ovaries to form persistent luteum, continuously secrete progesterone, inhibit follicular development and oestrus, and further limit the conception effect. At present, aiming at the dairy cows which are empty and have no oestrus behavior and are accompanied with persistent corpus luteum and uterus residues after being bred, a set of standardized and normalized breeding technical schemes integrating accurate judgment, targeted luteal dissolving, uterus purification, follicular accurate regulation and control, targeted twin-embryo promotion and efficient timed insemination are not formed. Disclosure of Invention The invention aims to overcome the defects of the prior art and provides a method for regulating and controlling and targeted inducing nonpregnant non-oestrus cows, which integrates the functions of accurate judgment, targeted dissolving of corpus luteum, uterine purification, follicular regulation and control, efficient timed insemination and targeted promotion of twin fetuses. The method has the advantages of high conception rate, high double-calf rate, simple operation, high safety and suitability for large-scale pasture application. The invention is suitable for dairy cows which have no oestrus behavior in 37 days after hybridization and are confirmed to be nonpregnant through ultrasonic examination, and the dairy cows have typical pathological states of early embryo death, lasting corpus luteum, residual embryo tissues in uterus and the like. The invention is realized by the following technical scheme that the method for regulating and controlling the induction of conception of the nonpregnant non-oestrus dairy cows is characterized by comprising the following steps: 1) Detecting LH and FSH levels and staging, determining luteinizing hormone LH and follicle stimulating hormone FSH levels in the peripheral blood or milk sample of dairy cows which have no oestrus behavior and are confirmed to be nonpregnant by ultrasonic examination after 37 days of hybridization, wherein the levels are divided into the following four stages according to the development degree of follicles: 11 FSH >2, LH <2, a dominant follicular recruitment phase; 12 When LH >2, FSH <2, dominant follicular dominant phase stage; 13 When LH >2 and FSH >2, is the preovulatory phase of follicular maturation; 14 When LH <2 and FSH <2, are embryonic residues, dominant luteal function, follicular resting phase; 2) Regulating and targeting induction of cows with different LH and FSH stage levels: 21 For all cows with LH <2, FSH <2, and embryo residue, dominant luteal function and follicular resting stage, intramuscular injection of prostaglandin PGF2 a + oxytocin + gonadotrophin releasing hormone GnRH is implemented, complete dissolution of the corpu