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CN-122005758-A - Active factor spray for skin inflammatory and allergic itch

CN122005758ACN 122005758 ACN122005758 ACN 122005758ACN-122005758-A

Abstract

The application relates to the technical field of biological medicines, and particularly discloses an active factor spray for inflammatory and allergic itch of skin. Is prepared from hyaluronic acid, lycorine N-demethylated derivative, oldenlandia diffusa polysaccharide, purified water and exosome mixed peptide according to a specific proportion. The exosome mixed peptide is composed of plant exosome loaded blue copper peptide, palmitoyl pentapeptide-4 and FGF2 growth peptide, so that a high-efficiency targeting delivery system is formed. The application combines the multicomponent compounding and the delivery technology to cooperatively play the roles of anti-inflammatory, antipruritic and skin barrier repairing. Provides a safer and comprehensive new non-hormonal local treatment choice for skin inflammatory and allergic itch.

Inventors

  • CHEN DIJIANG

Assignees

  • 四川陈三六生物医药有限公司

Dates

Publication Date
20260512
Application Date
20260227

Claims (10)

  1. 1. An active factor spray for inflammatory and allergic itch of skin is characterized by being prepared from the following raw materials, by weight, 10-30 parts of hyaluronic acid, 10-20 parts of lycorine N-demethylated derivative, 5-15 parts of oldenlandia diffusa polysaccharide, 30-50 parts of purified water and 0.1-5 parts of exosome mixed peptide.
  2. 2. The active factor spray for inflammatory and allergic itch of skin according to claim 1, wherein said exosome mixed peptide is a mixture of mixed peptide and exosome, wherein said mixed peptide is blue copper peptide, palmitoyl pentapeptide-4 and FGF2 growth peptide.
  3. 3. An active factor spray for use in inflammatory and allergic itching of skin according to claim 2, wherein said exosomes are plant exosomes.
  4. 4. An active factor spray for inflammatory and allergic itch of skin according to claim 3, wherein said plant exosomes are acanthopanax exosomes.
  5. 5. The active factor spray for inflammatory and allergic itch of skin according to claim 1, wherein the preparation method of the lycorine N-demethylated derivative comprises the steps of reacting lycorine and boron tribromide in a methylene dichloride solvent with the weight being 8-10 times of that of lycorine according to the molar ratio of 1:3-1:4 at the temperature of-10-0 ℃ under the conditions of nitrogen protection and magnetic stirring speed of 250-350rpm for 8-12 hours, slowly adding ice water for quenching reaction after the reaction is finished, adjusting the pH value to 8-9 by using sodium hydroxide solution, separating liquid to obtain an organic phase, drying by anhydrous sodium sulfate, and purifying by column chromatography, wherein a silica gel column eluent is methylene dichloride: methanol=10:1, thus obtaining the lycorine N-demethylated derivative.
  6. 6. The active factor spray for treating skin inflammatory and allergic itch according to claim 1, wherein the exosome mixed peptide is prepared by respectively dissolving blue copper peptide, palmitoyl pentapeptide-4 and FGF2 growth peptide in PBS according to a mass ratio of 1:1:1, mixing, adding acanthopanax exosome suspension, and performing low-voltage electroporation combined with short-time sonic treatment.
  7. 7. The spray of active factors for inflammatory and allergic itch of skin according to claim 4, wherein said acanthopanax exosomes are prepared by selecting fresh acanthopanax Pi Genpi, removing impurities, cutting into pieces, soaking in PBS, incubating at low temperature, filtering, and centrifuging.
  8. 8. An active factor spray for use in inflammatory and allergic itching of skin according to claim 7 wherein said low temperature incubation is in particular 4 ℃ low temperature incubation for 12 hours.
  9. 9. An active factor spray for inflammatory and allergic itch of skin according to claim 1, wherein said active factor spray is prepared by the process of: Preparing a water phase base solution, namely adding purified water into a sterile reaction kettle, stirring at a low speed, slowly adding the sieved hyaluronic acid, stirring while adding to prevent agglomeration, and continuing stirring until the hyaluronic acid is completely dissolved to obtain a hyaluronic acid water phase solution; Dissolving spreading hedyotis herb polysaccharide, namely keeping the stirring rate of a reaction kettle, slowly adding the screened spreading hedyotis herb polysaccharide into the hyaluronic acid aqueous phase liquid in the step (1), and continuously stirring to obtain the spreading hedyotis herb hyaluronic acid aqueous phase liquid; Adding lycorine N-demethylated derivative, stirring until no obvious particle exists, adding exosome mixed peptide, and continuing stirring to fully fuse peptide components with the system to obtain composite stock solution; homogenizing and filtering, namely transferring the composite stock solution into a high-pressure homogenizer, homogenizing, filtering and sterilizing by a microporous filter membrane, and collecting the filtered clarified stock solution to obtain a spray semi-finished product; and (3) filling and sealing, namely filling the finished product liquid into a spray bottle in an aseptic filling environment, immediately screwing an atomization nozzle, and sealing to obtain a spray finished product.
  10. 10. An active factor spray for inflammatory and allergic itch of skin according to claim 9, wherein said microporous filter membrane of step (4) has a pore size of 0.45 μm and 0.22 μm.

Description

Active factor spray for skin inflammatory and allergic itch Technical Field The application belongs to the technical field of biological medicines, and particularly relates to an active factor spray for inflammatory and allergic itch of skin. Background Skin itch is one of the most common clinical symptoms of various inflammatory and allergic diseases, such as atopic dermatitis, contact dermatitis, eczema, urticaria and the like, and the occurrence mechanism is complex, and the release and nerve sensitization process of various mediators, such as histamine, neuropeptides, cytokines and the like are involved. The chronic or recurrent itching not only severely affects the quality of life of the patient, but also can further damage the skin barrier due to scratching, exacerbating the inflammatory response, and forming a "itching-scratching" vicious circle. The topical antipruritic agents commonly used in clinic at present mainly comprise glucocorticoids, calcineurin inhibitors, antihistamines, local anesthetics and the like. Although the medicines can relieve symptoms to a certain extent, the application of the medicines still has obvious limitations that the glucocorticoid can cause adverse reactions such as skin atrophy, telangiectasia and the like after long-term use, the calcineurin inhibitor can have potential long-term safety concerns, the oral antihistamine is often accompanied with central nervous system side effects such as somnolence, hypodynamia and the like, the traditional local antipruritic preparation is often remained on the surface, the regulation and control on deep inflammation and nerve sensitization are insufficient, and the effect of repairing the skin barrier function is limited. In recent years, there have been studies on the use of bioactive peptides (e.g., blue copper peptide, growth factor) or plant extracts for skin repair, but these components alone have problems such as low transdermal absorption rate, easy inactivation in skin environment, and difficulty in targeted delivery to a desired action level. The components are singly used for external application and have the problems of low transdermal absorption rate, easy inactivation in skin environment, difficult targeted delivery and the like. Although exosomes are paid attention to as novel delivery vehicles, the exosomes are used for cooperatively loading various functional peptides (such as blue copper peptide, palmitoyl pentapeptide-4 and FGF2 growth peptide) and are compounded with lycorine derivatives, oldenlandia diffusa polysaccharide and other components to construct a multi-target and multi-mechanism cooperative 'itching relieving-anti-inflammatory-repairing' integrated local spray preparation, which has not been reported at present. In addition, the lycorine N-demethylated derivative has specific anti-inflammatory and neuromodulation activities, is scientifically proportioned with oldenlandia diffusa polysaccharide with immunoregulation function and high-efficiency moisturizing hyaluronic acid, and is physically integrated with specific functional peptide through exosome technology (such as specific plant exosomes), so that a multi-target and multi-mechanism synergistic 'itching relieving-anti-inflammatory-repairing' integrated local spray preparation is constructed, which is still blank in the prior art. Disclosure of Invention Aiming at the problems of large side effect, limited repairing effect and low active ingredient delivery efficiency of the traditional medicines in the treatment of skin inflammatory and allergic itch, the application provides an active factor spray for skin inflammatory and allergic itch, which is prepared by compounding hyaluronic acid, lycorine N-demethylated derivative and oldenlandia diffusa polysaccharide, combining plant exosomes loaded with blue copper peptide, palmitoyl pentapeptide-4 and FGF2 growth peptide to form a compound delivery system, and preparing a spray by matching with an optimized preparation process, thereby realizing multi-mechanism synergy, high-efficiency transdermal and long-acting repairing, and providing a safer and more comprehensive novel local treatment scheme. The application provides an active factor spray for inflammatory and allergic itch of skin, which is prepared from the following raw materials, by weight, 10-30 parts of hyaluronic acid, 10-20 parts of lycorine N-demethylated derivative, 5-15 parts of oldenlandia diffusa polysaccharide, 30-50 parts of purified water and 0.1-5 parts of exosome mixed peptide. Preferably, the exosome mixed peptide is a mixture of mixed peptide and exosome, wherein the mixed peptide is blue copper peptide, palmitoyl pentapeptide-4 and FGF2 growth peptide. Preferably, the exosomes are plant exosomes. Preferably, the plant exosome is an acanthopanax exosome. Preferably, the preparation method of the lycorine N-demethylated derivative comprises the steps of reacting lycorine and boron tribromide in a molar ratio of 1:3-1:4 in a d