CN-122005765-A - Application of B2UKX protein in preparation of anti-aging preparation

Abstract

The invention belongs to the field of biological medicine, and discloses an application of B2UKX protein in preparation of an anti-aging preparation, wherein the anti-aging preparation is a medicine for improving lumbar vertebra, muscle, kidney or intestinal tract aging. The invention discovers for the first time that the B2UKX5 protein can obviously delay disc degeneration, improve intestinal barrier function, improve muscle aging and alleviate kidney aging, is suitable for preventing and treating a plurality of aging-related systems such as lumbar vertebra, muscle, kidney, intestinal tract and the like, and has wide application prospect in preparing anti-aging preparations.

Inventors

• XIE HUI
• GUAN ZHE
• WANG ZHENXING
• CHEN CHUNYUAN
• LI XIAOXUE
• LIU JIANGHUA

Assignees

• 中南大学湘雅医院

Dates

Publication Date

20260512

Application Date

20251105

Claims (3)

1. 1. The application of the B2UKX protein in preparing an anti-aging preparation is characterized in that the protein sequence of the B2UKX protein is shown as SEQ ID NO. 1.
2. 2. The use according to claim 1, wherein the anti-aging agent is a drug for improving lumbar, muscle, kidney or intestinal aging.
3. 3. The use according to claim 1, wherein the B2UKX protein is expressed in competent cells BL21 DE3 from the B2UKX5 gene as shown in SEQ ID No. 2.

Description

Application of B2UKX protein in preparation of anti-aging preparation Technical Field The invention belongs to the technical field of biological medicines, and particularly relates to application of B2UKX protein in preparation of medicines for improving aging of lumbar vertebrae, muscles, kidneys or intestinal tracts and the like. Background Senescence is a complex process of progressive deterioration of multiple system functions involving mechanisms such as inflammation upregulation, mitochondrial dysfunction, extracellular matrix degeneration, and stem cell depletion. At present, a broad-spectrum, safe and efficient anti-aging intervention means is not available. The research shows that intestinal microecology plays a core role in aging regulation and control, and the intestinal microecology participates in the maintenance of the functions of the remote tissues through mechanisms such as an intestinal-lumbar axis, an intestinal-muscular axis, an intestinal-renal axis and the like. However, there is no powerful means for preventing and treating a plurality of aging-related systems such as lumbar vertebrae, muscles, kidneys and intestinal tracts. Disclosure of Invention The invention aims to overcome the defects and the shortcomings in the background art and provide the effect of B2UKX protein in delaying multi-system aging. In order to solve the technical problems, the technical scheme provided by the invention is as follows: The application of the B2UKX protein in preparing an anti-aging preparation is disclosed, wherein the protein sequence of the B2UKX protein is shown as SEQ ID NO. 1. The application further comprises that the anti-aging preparation is a medicine for improving lumbar vertebra, muscle, kidney or intestinal aging. Further, the B2UKX protein is obtained by expressing a B2UKX5 gene shown as SEQ ID NO.2 in competent cells BL21 DE 3. Compared with the prior art, the invention has the beneficial effects that: the invention discovers for the first time that the B2UKX protein can obviously delay disc degeneration, improve intestinal barrier function, improve muscle aging and alleviate kidney aging, is suitable for preventing and treating a plurality of aging-related systems such as lumbar vertebra, muscle, kidney, intestinal tract and the like, and has wide application prospect. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. FIG. 1 shows the effect of B2UKX on delaying intestinal aging in a natural aging model, wherein (A) proximal and distal H & E staining of the colon after treatment with B2UKX5 shows macroscopic morphology, (B) proximal and distal periodate snow staining of the colon after treatment with B2UKX5, (C) MUC2 immunohistochemical representative image, (D) Occludin immunohistochemical representative image; FIG. 2 is the effect of B2UKX on delaying renal aging in a natural aging model; FIG. 3 is a schematic illustration of the experimental procedure for B2UKX to delay lumbar aging in a model of natural aging, wherein (A) B2UKX5 treatment in mice with natural aging; A T2 weighted MRI image of the mouse lumbar (scale: 1 mm) after B2UKX treatment, (C) a statistical analysis of the average PFIRRMANN grade of the L1-S1 lumbar segments (n=9-14 per group), (D) a micro-CT three-dimensional reconstructed image of the L5-6 lumbar segments (scale: 1 mm), (E) a statistical analysis of the average Disc Height Index (DHI) of the L2-6 lumbar segments (n=8-10 per group), (F) H & E staining shows macroscopic morphology of the L5-6 lumbar segments Disc (Disc), nucleus Pulposus (NP), annulus Fibrosus (AF) and End Plates (EP) (scale: 200 μm (Disc) or 50 μm (NP, AF, EP)), (G) SafraninO-FASTGREEN staining shows macroscopic morphology of the L5-6 lumbar segments Disc (Disc), nucleus Pulposus (NP), annulus Fibrosus (AF) and End Plates (EP) (scale: 200 μm (Disc) or 50 μm (NP, AF, EP) each (4-4L 2-6 lumbar segments (4L 2-4) and (4-5-6L 2-4 m (4)) ACAN and representative immunofluorescence images of MMP13 expression (ruler: 200 μm), (J) quantitative analysis of COL2, ACAN and MMP13 expression (n=8-10 per group), data expressed as mean ± standard deviation, statistical significance assessed by student t test (B) or single factor anova combined with Bonferroni post hoc test (E, G, J, L); FIG. 4 shows the effect of B2UKX on delaying muscle aging in a model of natural aging, wherein (A) the tibialis anterior muscle H & E of each group is stained to show the morphology and area of the muscle fibers after treatment with B2UKX5, and (B) the statistical analysis of the cross-sectional area of t