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CN-122005790-A - Photosensitizer/ZIF-8 nano-composite, preparation method and application thereof, and pharmaceutical composition for treating periodontitis and/or peri-implant inflammation

CN122005790ACN 122005790 ACN122005790 ACN 122005790ACN-122005790-A

Abstract

The application provides a photosensitizer/ZIF-8 nano-composite, a preparation method and application thereof, and a pharmaceutical composition for treating periodontitis and/or peri-implant inflammation. The photosensitizer/ZIF-8 nano-composite is prepared by the preparation method, and the preparation method comprises the steps of dissolving zinc salt in a solvent to form solution A, dissolving imidazole ligand in the solvent, adding the photosensitizer under the condition of light shielding to form solution B, quickly adding the solution A into the solution B under the condition of light shielding, stirring the solution A at room temperature for reaction, centrifuging the solution A after the reaction is finished to obtain a solid product, washing the solid product, and drying the solid product. The photosensitizer/ZIF-8 nanocomposite is applied to preparation of photodynamic antibacterial drugs. The pharmaceutical composition for treating periodontitis and/or peri-implant inflammation comprises the photosensitizer/ZIF-8 nanocomposite and auxiliary materials. The preparation method is simple, the raw materials are cheap and easy to obtain, and the obtained compound has obvious antibacterial effect and low toxicity when being used as a photodynamic antibacterial drug.

Inventors

  • WANG DONGQING
  • TANG WENJUN

Assignees

  • 首都医科大学附属北京口腔医院

Dates

Publication Date
20260512
Application Date
20260214

Claims (10)

  1. 1. A method for preparing a photosensitizer/ZIF-8 nanocomposite, comprising the steps of: S1, zinc salt is dissolved in a solvent and uniformly dispersed to form solution A; s2, dissolving imidazole ligand in a solvent, adding a photosensitizer under the light-shielding condition, and uniformly dispersing to form solution B; S3, under the light-shielding condition, rapidly adding the solution A into the solution B, and stirring and reacting for a certain time at room temperature; and S4, carrying out centrifugal separation after the reaction is finished in S3 to obtain a solid product, and washing and drying the solid product to obtain the photosensitizer/ZIF-8 nanocomposite.
  2. 2. The method of preparing a photosensitizer/ZIF-8 nanocomposite according to claim 1, wherein the zinc salt in step S1 comprises zinc nitrate hexahydrate, zinc acetate dihydrate; And/or the number of the groups of groups, The imidazole ligand in the step S2 comprises 2-methylimidazole; And/or the number of the groups of groups, The solvents in the step S1 and the step S2 are methanol; And/or the number of the groups of groups, The photosensitizer is rose bengal.
  3. 3. The method for preparing the photosensitizer/ZIF-8 nanocomposite according to claim 2, wherein the mass ratio of the zinc nitrate hexahydrate to the 2-methylimidazole to the rose bengal is 50-200:110-440:5-40.
  4. 4. The preparation method of the photosensitizer/ZIF-8 nanocomposite according to claim 1, wherein the uniform dispersion in the step S1 and the step S2 comprises ultrasonic dispersion for 5-10 min; And/or the number of the groups of groups, The stirring reaction time in the step S3 is 5-25 min; And/or the number of the groups of groups, In the step S4, the washing is performed using the solvent; And/or the number of the groups of groups, And the drying is carried out at 50-60 ℃ for 8-24 hours.
  5. 5. The method for preparing photosensitizer/ZIF-8 nanocomposite according to claim 4, wherein the washing further comprises adding a proper amount of methanol to the solid product obtained by centrifugal separation, shaking up, performing centrifugal washing, continuing adding a proper amount of methanol to the precipitate obtained by centrifugal washing, shaking up again, performing centrifugal washing again, and repeating the centrifugal washing for 2-3 times; And/or the number of the groups of groups, The drying further comprises the step of preserving the temperature of the washing product for 2-3 hours at 35-40 ℃ under the vacuum condition before the vacuum drying at 50-60 ℃.
  6. 6. A photosensitizer/ZIF-8 nanocomposite prepared by the preparation method according to any one of claims 1 to 5, characterized in that the said composition is a rose bengal/ZIF-8 nanocomposite.
  7. 7. The photosensitizer/ZIF-8 nanocomposite according to claim 6, wherein the loading rate of the photosensitizer is 10-30 wt% and the encapsulation rate is 55-70%; And/or the number of the groups of groups, The particle size of the nano composite is 160-320 nm.
  8. 8. Use of the photosensitizer/ZIF-8 nanocomposite of claim 6 or 7, wherein the rose bengal/ZIF-8 nanocomposite is used for the preparation of photodynamic antibacterial drugs or oral care products; The photodynamic antibacterial drug or the oral care product can act on any one or more of porphyromonas gingivalis, actinobacillus conglomerates, fusobacterium nucleatum and praecox intermedia.
  9. 9. A pharmaceutical composition for treating periodontitis and/or peri-implantitis, characterized in that it comprises a photosensitizer/ZIF-8 nanocomposite according to claim 6 or 7, and an adjuvant.
  10. 10. The pharmaceutical composition of claim 9, wherein the pharmaceutical composition comprises any one or more of a gel for intra-periodontal pocket injection, a slow release fiber, a microsphere, or a degradable patch; And/or the number of the groups of groups, The gel takes carbomer, hyaluronic acid or poloxamer as a matrix; And/or the number of the groups of groups, The concentration of the rose bengal/ZIF-8 nano-composite in the pharmaceutical composition is 0.1-5.0 mg/mL.

Description

Photosensitizer/ZIF-8 nano-composite, preparation method and application thereof, and pharmaceutical composition for treating periodontitis and/or peri-implant inflammation Technical Field The application belongs to the technical field of photosensitive composite materials, and particularly relates to a photosensitizer/ZIF-8 nano-composite, a preparation method and application thereof, and a pharmaceutical composition for treating periodontitis and/or peri-implant inflammation. Background Periodontitis is a common infectious disease of the oral cavity, and its major pathogenic bacteria include Acidovorax faciens, fusobacterium nucleatum, proteus intermedia, and Porphyromonas gingivalis. The traditional mechanical treatment commonly used in clinic has poor treatment effect on the parts such as complicated anatomy, teeth Zhou Daishen and the like, and meanwhile, the use of antibiotics is easy to cause drug resistance. Photodynamic therapy (Photodynamic Therapy, PDT) has received considerable attention in recent years as a non-invasive, non-induced drug resistance, targeted antimicrobial strategy. The core of photodynamic therapy is a photosensitizer, which is utilized to generate Reactive Oxygen Species (ROS) under specific wavelength illumination, so as to kill microorganisms, and is a novel strategy for periodontal disease non-antibiotics. Rose Bengal (RB) is a typical high-efficiency photosensitizer with high singlet oxygen quantum yield and photostability, but its poor dispersibility in water, poor targeting to bacteria, easy aggregation quenching, high toxicity to host cells, limiting its application. The metal organic framework material ZIF-8 is used for drug delivery due to its high specific surface area and pH response degradation characteristics. There has been recent study of RB loading on ZIF-8 for E.coli or skin infection, but its application to oral pathogenic multi-bacterial biofilm model has not been seen. More importantly, the oral clinic lacks special green light equipment, a main peak of 450-470 nm blue light curing lamp is mainly used, and the maximum absorption of RB is located at 540-560 nm, so that the excitation efficiency is challenging. Therefore, the RB/ZIF-8 compound which can effectively remove oral multi-bacterial biomembrane under the common narrow-spectrum blue light source and has good tissue compatibility is developed, and the compound has important practical value. In view of this, the present invention has been made. Disclosure of Invention The application aims to provide a photosensitizer/ZIF-8 nano-composite, a preparation method and application thereof and a pharmaceutical composition for treating periodontitis and/or peri-implant inflammation, so as to solve the problems. In order to achieve the purposes of the application, the technical scheme adopted by the application is as follows: in a first aspect, the present application provides a method for preparing a photosensitizer/ZIF-8 nanocomposite, the method comprising the steps of: s1, dissolving zinc salt in a solvent, and uniformly dispersing to form solution A, wherein the zinc salt preferably comprises zinc nitrate hexahydrate and zinc acetate dihydrate, and the solvent is methanol; S2, dissolving imidazole ligand in a solvent, adding a photosensitizer under a light-shielding condition, and uniformly dispersing to form solution B, wherein the imidazole ligand preferably comprises 2-methylimidazole, and the solvent is preferably methanol; Preferably, the photosensitizer is rose bengal; Further preferably, the mass ratio of the zinc nitrate hexahydrate to the 2-methylimidazole to the rose bengal is 50-200:110-440:5-40; optionally, the mass ratio of the zinc nitrate hexahydrate to the 2-methylimidazole to the rose bengal is 150:330:5-40; Further, the mass ratio of zinc nitrate hexahydrate, the 2-methylimidazole, the rose bengal may be any value between 150:330:5, 150:330:10, 150:330:15, 150:330:20, 150:330:25, 150:330:30, 150:330:35, 150:330:40. Preferably, the uniform dispersion in the step S1 and the step S2 comprises ultrasonic dispersion for 5-10 min; S3, under the condition of avoiding light, the solution A is rapidly added into the solution B, and stirring reaction is carried out for a certain time at room temperature, preferably, the stirring reaction time is 5-25 min; and S4, carrying out centrifugal separation after the reaction is finished in S3 to obtain a solid product, and washing and drying the solid product to obtain the photosensitizer/ZIF-8 nanocomposite. In the step S4, the washing is performed by using the solvent, preferably, the washing further comprises adding a proper amount of solvent into the solid product obtained by centrifugal separation, shaking uniformly, performing centrifugal washing, continuously adding a proper amount of solvent into the precipitate obtained by centrifugal washing, shaking uniformly again, performing centrifugal washing again, and repeating the centrifugal washing for