CN-122005795-A - Curcumin-based carbon-point nano enzyme cascade catalytic system and application thereof in targeted anticariogenic bacteria

Abstract

The invention discloses a curcumin-based carbon dot nano enzyme cascade catalytic system and application thereof in targeting anticariogenic bacteria, belonging to the technical field of biological medicine, wherein the catalytic system is prepared by mixing curcumin-based carbon dot nano enzymes Cur@Fe, mn/CDs and glucose oxidase GOx, performing ultrasound and incubating; the curcumin-based carbon point nano enzyme cascade catalytic system shows near infrared enhanced peroxidase-like activity and excellent photo-thermal performance, hydrogen peroxide generated by GOx oxidation of glucose is used as a substrate of peroxide mimic enzymes Cur@Fe, mn/CDs, high-toxicity hydroxyl free radicals are generated in an acidic environment, the positions of biological membranes are precisely positioned by positive charges on the surfaces of nano enzymes and GOx, and the nano enzymes can target and inhibit cariogenic bacteria of S.mutans and S.sanguinis, and hardly influence the normal oral cavity S.oralis.

Inventors

• LIU JIAXIN
• LI JITAO
• LI ZIZHUO
• Gao Churan
• LI SHANGLIN
• YANG YALING

Assignees

• 昆明理工大学

Dates

Publication Date

20260512

Application Date

20260416

Claims (5)

1. 1. A curcumin-based carbon point nano enzyme cascade catalytic system is characterized in that curcumin-based carbon point nano enzymes Cur@Fe, mn/CDs, glucose oxidase GOx and the like are mixed by mass, subjected to ultrasonic treatment for 60-90min, and then incubated for 8-10h at 4 ℃ to prepare the curcumin-based carbon point nano enzyme cascade catalytic system; Wherein, the curcumin-based carbon point nano enzyme Cur@Fe and Mn/CDs are prepared by dissolving 0.5-0.8g curcumin in 1-2mL absolute ethyl alcohol, then adding 0.3-0.5g citric acid, 0.35-0.50g FeCl 3 ·6H 2 O、0.35-0.50g MnCl 2 ·4H 2 O and 30-50mL acetic acid aqueous solution with the weight-volume ratio g of 1-2%, carrying out ultrasonic treatment on the mixture for 20-30min, reacting for 100-150min at the microwave power of 1.0-1.2kW and the temperature of 170-190 ℃, cooling the reaction product, centrifuging, taking supernatant, filtering with a filter membrane with the thickness of 0.22mm, and carrying out vacuum drying.
2. 2. The application of the curcumin-based carbon dot nano-enzyme cascade catalytic system in preparing near infrared light enhanced targeted anticariogenic bacteria preparation.
3. 3. The application of the method according to claim 2, wherein the curcumin-based carbon nano enzyme cascade catalytic system is uniformly mixed with glucose, and the target anticariogenic bacteria is realized by irradiation for 10-15min under 808nm near infrared light when the method is used.
4. 4. The method according to claim 2, wherein the cariogenic bacteria are Streptococcus mutans (Streptococcus mutans) and Streptococcus sanguis (Streptococcus sanguinis).
5. 5. The method according to claim 3, wherein the power of the near infrared light at 808nm is 0.50-1.0W/cm 2 .

Description

Curcumin-based carbon-point nano enzyme cascade catalytic system and application thereof in targeted anticariogenic bacteria Technical Field The invention belongs to the technical field of biological medicine, and particularly relates to a curcumin-based carbon dot nano enzyme cascade catalytic system and application thereof in near infrared light enhanced targeting anticariogenic bacteria. Background Caries (DENTAL CARIES) or caries (DENTAL DECAY) is a disease that is stably present in enamel or hard tissue of the tooth, one of the most common chronic infectious diseases worldwide, with a prevalence of 35% in all age groups. Caries formation in human dental hard tissue is directly related to plaque biofilm in the oral environment. Notably, cariogenic bacteria such as Streptococcus mutans (Streptococcus mutans) are capable of producing acids and thus caries, and biofilms are embedded in Extracellular Polymeric Substances (EPS) matrices by well-structured microorganisms, which have structural stability and mechanically rigid protective barriers that can cause persistent human infections, and anoxic plaque biofilms also contain high concentrations of H 2O2. The primary goal of caries prevention is to clear recalcitrant plaque biofilm and reduce tooth demineralization. However, clinical mechanical stain removal, while effective in eliminating plaque biofilm, is inadequate to clean inaccessible areas and often causes damage to the tooth surface. Many synthetic agents, such as chlorhexidine, quaternary ammonium salts, fluoride, and high concentrations of H 2O2 (3%) have been widely used to clear bacteria in the oral cavity, disrupting the microflora balance. Thus, it is necessary to explore an integrated platform for specific capture, targeting and clearance of pathogens while minimizing damage to normal microbiota and surrounding tissues. Recently, research on peroxidase-like nano enzymes based on metal-doped Carbon Dots (CDs) in the antibacterial field has been increasing, and iron-doped carbon dots combine catalytic and optical properties of metals and size effects of quantum dots to effectively combat bacteria through synergistic effects with chemical kinetics, photothermal and photodynamic pathways, and at the same time, CDs have high photothermal conversion capability. When exposed to near infrared light (NIR), the surface electrons of CDs absorb light energy and perform a transition from the ground state to the excited state, and then back to the ground state again, the energy being released in the form of heat, creating a photo-thermal effect that kills bacteria by means of a rapidly increasing temperature, i.e. photo-thermal therapy (PTT). Thus, the class of peroxidases based on doped CDs provides the potential for a synergistic effect of photodynamic therapy (PDT) and PTT antibacterial mechanisms. The mechanism of action of PDT is that the photosensitizer is activated under laser irradiation to generate a large amount of cytotoxicity singlet oxygen (1O2) and superoxide radical (O 2·-) to remove the biofilm, while PTT utilizes the photothermal agent (PTAs) absorbed by near infrared light to generate local thermal therapy to remove the biofilm. Disclosure of Invention Based on the problems of the background technology, the invention provides a curcumin-based carbon-point nano enzyme cascade catalytic system and application thereof in preparing near infrared light enhanced targeted anticariogenic bacteria preparations, the invention prepares a multifunctional Fe, mn-doped curcumin-based carbon-point integrated glucose oxidase nano enzyme cascade catalytic system (Cur@Fe, mn/CDs@GOx), cur@Fe, mn/CDs@GOx shows near infrared enhanced peroxidase activity and excellent photo-thermal performance, hydrogen peroxide (H 2O2) generated by GOx oxidation glucose is used as a substrate of peroxide mimic enzyme Cur@Fe, mn/CDs, high toxic hydroxyl free radicals are generated in an acidic environment, and simultaneously, the position of a biological membrane is accurately positioned by utilizing positive charges and GOx on the surface of nano enzyme, the photo-promoted cascade catalytic system can remarkably improve the generation of active oxygen (ROS) and effectively eliminate the biological membrane, and the concentration of the biological membrane is proved by the invention, and the concentration of the biological membrane is improved to be 34% when the biological membrane is formed by the novel method, and the concentration of the biological membrane is 34% of the biological membrane, and the biological membrane is provided with the specific biological membrane has the toxicity of the biological membrane, and the toxicity of the biological membrane is 34% of the biological membrane. In order to achieve the above purpose, the invention is realized by the following technical scheme: 1. Dissolving 0.5-0.8g of curcumin in 1-2 mL absolute ethyl alcohol, sequentially adding 0.3-0.5g of citric acid, 0.35-0.50g of FeCl 3·6H