Search

CN-122005827-A - Application of CuOx copper material with antibacterial activity in preparation of antibacterial drugs

CN122005827ACN 122005827 ACN122005827 ACN 122005827ACN-122005827-A

Abstract

The invention belongs to the technical cross field of biomedicine and metal materials, and discloses an application of a CuOx copper material with antibacterial activity in preparation of antibacterial drugs, wherein the copper material is prepared from different carboxylic acids serving as modification ligands, bipyridine or phenanthroline and other dinitrogen ligands and a metal copper salt precursor under the hydrothermal condition, and has the size of 1-30 mu m and better biocompatibility. The copper material can realize high-efficiency inhibition of various bacteria under the condition of low concentration (5-10 mug/L). One of the copper materials has the best activity, has better inhibition capability on the attenuated strain Ra of the mycobacterium tuberculosis H37Rv, greatly increases the effect of the CuOx copper material on clinical practical application, and enriches the diversity of the CuOx copper material with antibacterial activity.

Inventors

  • WANG CHUN
  • HU YANGBO
  • JIANG XIAOLIN

Assignees

  • 湖北江夏实验室
  • 中国科学院武汉病毒研究所
  • 上海健康医学院

Dates

Publication Date
20260512
Application Date
20260306

Claims (10)

  1. 1. The application of the CuOx copper material with antibacterial activity in the preparation of antibacterial drugs is characterized in that the copper material is prepared from carboxylic acid ligands, bipyridine or phenanthroline and other dinitrogen ligands and copper precursors with different modifications, and sodium hydroxide is added at the same time under hydrothermal conditions, and the size of the copper material is 1-30 mu m.
  2. 2. The use according to claim 1, wherein the differently modified carboxylic acid ligands introduce substituents of a regulatable electronic nature selected from one or more of methyl, ethyl, hydroxyl, amino, fluoro, chloro, through regulating the ring body to be a benzene ring, pyridine or other heterocyclic ring and a group on the ring.
  3. 3. The use according to claim 1, wherein the copper precursor is a soluble copper salt selected from one or more of copper nitrate, copper chloride, copper sulphate, copper acetate and water of crystallization thereof.
  4. 4. The method according to claim 1, wherein the hydrothermal condition is a reaction temperature of 120-240 ℃ and a reaction time of 12-96 h.
  5. 5. The use according to claim 1, wherein the amount of sodium hydroxide is 0.1-5 times the amount of the dinitrogen ligand such as bipyridine or phenanthroline.
  6. 6. The use according to claim 1, characterized in that the morphology of the copper material is selected from the group consisting of blocks or blocks with pores, the dimensions of which vary from 1 μm to 30 μm.
  7. 7. The use according to claim 1, characterized in that the surface water-soluble, fat-soluble lipids of the copper material are regulated by the selection of different carboxylic acid ligands.
  8. 8. The use according to claim 1, wherein the antibacterial agent is for inhibiting gram positive bacteria, gram negative bacteria or mycobacterium tuberculosis.
  9. 9. The use according to claim 8, wherein said mycobacterium tuberculosis is mycobacterium tuberculosis H37Rv attenuated strain Ra.
  10. 10. The use according to claim 1, wherein the effective concentration of copper material in the antimicrobial medicament is 50 μg/ml or less.

Description

Application of CuOx copper material with antibacterial activity in preparation of antibacterial drugs Technical Field The invention belongs to the technical cross field of biomedicine and metal materials, and particularly relates to an application of a CuOx copper material with antibacterial activity in preparation of antibacterial drugs, which is particularly suitable for research and development of therapeutic drugs for various bacterial infections such as mycobacterium tuberculosis. Background In recent years, prevention and control of bacteria mainly depend on antibiotics, but abuse of antibiotics aggravates the generation of drug-resistant strains, so that the treatment effect of traditional antibiotics is gradually reduced, the problem of bacterial drug resistance is increasingly serious, and great challenges are brought to clinical infection treatment. Therefore, the development of novel, efficient and low-toxicity antibacterial drugs becomes a research hotspot in the current biomedical field. Copper-based materials are used as a metal material with enzyme catalytic activity, have good antibacterial performance, and the antibacterial mechanism of the copper-based materials can involve damage to microbial cell membranes, generation of Reactive Oxygen Species (ROS) by induced oxidative stress, inhibition of key enzyme activities, destruction of nucleic acid structures and the like. The unique physicochemical property and biological activity of the antibacterial agent are wide in source and low in cost, so that the antibacterial agent has good application prospect in the antibacterial field. However, the existing copper-based materials have the defects that firstly, the morphology and the size are difficult to accurately regulate and control in the synthesis process, so that the antibacterial activity is unstable, secondly, the properties such as surface hydrophilicity and hydrophobicity are single, biocompatibility and cell permeability are required to be improved, thirdly, the inhibition effect of most copper-based materials on intractable pathogenic bacteria such as mycobacterium tuberculosis is poor, and part of copper-based materials have the problems of high cytotoxicity and poor safety, so that the clinical application of the copper-based materials is limited. The carboxylic acid ligand has rich electronic structure, and the electronic property can be regulated and controlled by carrying out different modifications on the carboxylic acid ligand, so that the coordination capacity with metal ions is influenced. At present, there is no report on preparing copper-based materials with controllable morphology, size and surface properties by using different modified carboxylic acid ligands and bipyridine or phenanthroline and other dinitrogen ligands together and copper precursors through a hydrothermal method, and applying the copper-based materials to the preparation of antibacterial drugs. Therefore, a synthesis method for modifying bipyridine or phenanthroline and other dinitrogen ligand and metal salt precursor copper materials based on carboxylic acid ligand is developed, cuOx copper materials with high antibacterial activity and good biocompatibility are prepared, and the method has important significance for promoting innovation and development of antibacterial drugs. Disclosure of Invention The invention aims to provide an application of a CuOx copper material with antibacterial activity in preparation of antibacterial drugs, and the CuOx copper material has the advantages of controllable morphology and size, good biocompatibility, high antibacterial activity, low toxicity and the like through hydrothermal synthesis of carboxylic acid ligands modified differently and copper precursors, and particularly has a good inhibition effect on mycobacterium tuberculosis. In order to achieve the above object, the present invention can be achieved by the following technical scheme: The application of CuOx copper material with antibacterial activity in preparing antibacterial drugs is characterized in that the copper material is prepared by taking different carboxylic acids as modification ligands, bipyridine or phenanthroline and other dinitrogen ligands and copper precursors, and simultaneously adding sodium hydroxide under hydrothermal conditions, wherein the size of the copper material is 1-30 mu m. Further, the different carboxylic acids as the modifying ligand introduce substituents of controllable electronic properties selected from one or more of methyl, ethyl, hydroxy, amino, fluoro, chloro through the control ring body being a benzene ring, pyridine or other heterocyclic ring and a group on the ring. Further, the copper precursor is a soluble copper salt including, but not limited to, copper nitrate, copper chloride, copper sulfate, copper acetate, crystalline water forms thereof, and the like. Further, the hydrothermal conditions are that the reaction temperature is 120-240 o ℃ and the reaction t