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CN-122005831-A - Exosome co-delivery composition and preparation method and application thereof

CN122005831ACN 122005831 ACN122005831 ACN 122005831ACN-122005831-A

Abstract

The invention belongs to the technical field of biology, and particularly relates to an exosome co-delivery composition, a preparation method and application thereof. The invention provides an exosome co-delivery composition, wherein a system A utilizes mannitol-chitosan oligosaccharide to embed active ingredients exosome and probiotics to construct a chitosan oligosaccharide-mannitol nano-encapsulation system, so that the survival rate and structural stability of the active ingredients can be improved, chitosan oligosaccharide in the system B forms a three-dimensional nano-gel network through ion crosslinking, butyrate and glutathione are embedded to form a stable nano-gel matrix, and a chitosan oligosaccharide-glutathione-butyrate nano-gel system is constructed, so that the multipath protection, targeted release and synergistic interaction of the probiotics, exosome, butyrate and glutathione are realized cooperatively, and a remarkable comprehensive promotion effect is generated in aspects of intestinal barrier repair, flora reconstruction, anti-inflammatory and antioxidation and energy supply.

Inventors

  • ZHANG LIANGTAO

Assignees

  • 长沙泌润生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260310

Claims (10)

  1. 1. An exosome co-delivery composition, wherein the exosome co-delivery composition comprises a system a and a system B; the system A comprises, by mass, 1-30 parts of exosome preparation, 1-60 parts of chitosan oligosaccharide-mannitol and 1-30 parts of probiotics, wherein the chitosan oligosaccharide-mannitol is used for embedding exosome and probiotics; The system B comprises, by mass, 1-60 parts of chitosan oligosaccharide, 1-20 parts of glutathione and 1-20 parts of butyrate, wherein the chitosan oligosaccharide is used for embedding the glutathione and the butyrate.
  2. 2. The exosome co-delivery composition according to claim 1, wherein the mass ratio of system a to system B is 1:1 to 10:1.
  3. 3. The exosome co-delivery composition according to claim 1, wherein the mass ratio of chitosan oligosaccharide to mannitol in the chitosan oligosaccharide-mannitol is 10:1-20:1.
  4. 4. A method of preparing an exosome co-delivery composition according to any one of claims 1 to 3, comprising the steps of: Mixing the exosome preparation, probiotics and water to obtain a mixed solution, and dropwise adding the mixed solution into a chitosan oligosaccharide-mannitol solution to perform self-assembly to obtain a system A; mixing said system a and said system B to obtain said exosome co-delivery composition.
  5. 5. The preparation method of claim 4, wherein the concentration of chitosan in the chitosan oligosaccharide-mannitol solution is 10-20 mg/mL and the concentration of mannitol is 1-2 mg/mL.
  6. 6. The preparation method of claim 5, wherein the step of adding the mixture into the chitosan oligosaccharide-mannitol solution drop by drop for self-assembly further comprises the step of adjusting the concentration of the chitosan oligosaccharide to 1-5 mg/mL.
  7. 7. The preparation method of claim 4, wherein the concentration of chitosan oligosaccharide in the chitosan oligosaccharide solution is 10-20 mg/mL.
  8. 8. The preparation method of claim 7, wherein the self-assembly is carried out by adding butyrate solution and glutathione solution into chitosan oligosaccharide solution drop by drop, and the preparation method further comprises the step of adjusting the concentration of chitosan oligosaccharide to 1-5 mg/mL.
  9. 9. The preparation method according to claim 4, wherein the self-assembly method comprises stirring, wherein the rotation speed of stirring is 250-1000 rpm, and the stirring time is 2-4 h.
  10. 10. Use of an exosome co-delivery composition according to any one of claims 1 to 3 or an exosome co-delivery composition prepared by a preparation method according to any one of claims 4 to 9 in the preparation of a medicament for treating intestinal inflammation.

Description

Exosome co-delivery composition and preparation method and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to an exosome co-delivery composition, a preparation method and application thereof. Background Intestinal inflammation is a common digestive system disease, including chronic gastroenteritis, intestinal leakage and other various diseases, and the pathogenesis of the intestinal inflammation is closely related to intestinal flora imbalance, intestinal barrier damage, inflammatory reaction abnormality and the like. At present, bioactive substances such as probiotics, exosomes and the like show good application potential in intestinal inflammation treatment, but the active substances have the problem of poor stability, are easily damaged in the severe environment of gastrointestinal tracts such as gastric acid, pancreatin and the like, have low oral bioavailability, and are difficult to fully play a role in treatment. For example, chinese patents CN120078157a and CN118592600a are single delivery systems in which chitosan and probiotic, exosome bioactive substances are simply mixed, and their gastrointestinal repair effect is not good. Thus, there is a need for a composition that can improve the stability of the active substance and achieve targeted release. Disclosure of Invention The invention aims to provide an exosome co-delivery composition, a preparation method and application thereof, wherein the exosome co-delivery composition can realize targeted release and synergistic effect of multiple active ingredients, and provides a new solution for treating intestinal inflammation. The invention provides an exosome co-delivery composition, which comprises a system A and a system B; the system A comprises, by mass, 1-30 parts of exosome preparation, 1-60 parts of chitosan oligosaccharide-mannitol and 1-30 parts of probiotics, wherein the chitosan oligosaccharide-mannitol is used for embedding exosome and probiotics; The system B comprises, by mass, 1-60 parts of chitosan oligosaccharide, 1-20 parts of glutathione and 1-20 parts of butyrate, wherein the chitosan oligosaccharide is used for embedding the glutathione and the butyrate. As a preferable scheme, the mass ratio of the system A to the system B is 1:1-10:1. As a preferable scheme, the mass ratio of the chitosan oligosaccharide to the mannitol in the chitosan oligosaccharide-mannitol is 10:1-20:1. The invention also provides a preparation method of the exosome co-delivery composition, which comprises the following steps: Mixing the exosome preparation, probiotics and water to obtain a mixed solution, and dropwise adding the mixed solution into a chitosan oligosaccharide-mannitol solution to perform self-assembly to obtain a system A; mixing said system a and said system B to obtain said exosome co-delivery composition. As a preferable scheme, the concentration of the chitosan oligosaccharide in the chitosan oligosaccharide-mannitol solution is 10-20 mg/mL, and the concentration of the mannitol is 1-2 mg/mL. As a preferable scheme, the mixed solution is added into the chitosan oligosaccharide-mannitol solution drop by drop for self-assembly, and the method further comprises the step of adjusting the concentration of the chitosan oligosaccharide to 1-5 mg/mL. As a preferable scheme, the concentration of the chitosan oligosaccharide in the chitosan oligosaccharide solution is 10-20 mg/mL. As a preferable scheme, the method comprises the step of dropwise adding a butyrate solution and a glutathione solution into a chitosan oligosaccharide solution for self-assembly, and the step of adjusting the concentration of the chitosan oligosaccharide to 1-5 mg/mL. As a preferable scheme, the self-assembly method comprises stirring, wherein the rotating speed of stirring is 250-1000 rpm, and the stirring time is 2-4 hours. The invention also provides an application of the exosome co-delivery composition prepared by the scheme or the preparation method in preparing medicines for treating intestinal inflammation. The exosome co-delivery composition has the beneficial effects that the exosome co-delivery composition comprises a system A and a system B, the system A comprises, by mass, 1-30 parts of exosome preparation, 1-60 parts of chitosan-mannitol and 1-30 parts of probiotics, the chitosan-mannitol is used for embedding exosome and probiotics, and the system B comprises, by mass, 1-60 parts of chitosan, 1-20 parts of glutathione and 1-20 parts of butyrate, and the chitosan is used for embedding glutathione and butyrate. The system A utilizes mannitol-chitosan oligosaccharide to embed an active ingredient exosome and probiotics to construct a chitosan oligosaccharide-mannitol nano-package system, so that the survival rate and structural stability of the active ingredient can be improved, mannitol is used as a biological protective agent to inhibit membrane protein denaturation, reduce osmotic stres