CN-122005859-A - Application of FKBP12B in improving silkworm survival rate and silk quality

Abstract

The invention discloses application of FKBP12B in improving silkworm survival rate and silk quality. The invention discovers that after FKBP12B DNA is given to silkworms for the first time, the survival rate, cocoon shell weight, cocoon layer rate and silk quality of silkworms can be improved, and the FKBP12B has potential application value of improving the silk quality.

Inventors

• LI SI

Assignees

• 浙江理工大学

Dates

Publication Date

20260512

Application Date

20251222

Claims (10)

1. Use of the fkbp12b gene or a biomaterial related thereto in at least one of the following: a1 Improving the survival rate of silkworms; A2 Improving cocoon layer yield of silkworms; a3 Improving silk quality; A4 Preparing a product for improving the survival rate of silkworms; A5 Preparing a product for improving the cocoon layer yield of silkworms; A6 Preparing a product for improving the quality of silk.
2. 2. The use as claimed in claim 1, wherein the nucleotide sequence of FKBP12B gene comprises the sequence shown in SEQ ID No. 1.
3. 3. The use according to claim 2, wherein the silk quality comprises one or more of cocoon moisture regain, water content, cocoon gel content, silk weight, length and titer; and/or, the cocoon layer yield comprises cocoon shell weight and/or cocoon layer rate.
4. 4. The method of claim 1, wherein the biological material related to the biological material is selected from one or more of a protein, a recombinant expression vector or a bioengineered bacterium.
5. 5. The use according to claim 4, wherein the amino acid sequence of the protein comprises the sequence shown in SEQ ID NO. 1.
6. 6. The use according to claim 4, wherein the starting plasmid of the recombinant expression vector is selected from one of pIEx-1, pIEx-2, pIEx-3, pIEx-4, pTriEx-1.1, pTriEx-2 and pDal-IC.
7. 7. The use according to any one of claims 1 to 6, wherein the FKBP12B gene or biomaterial associated therewith is used alone or in combination with other drugs.
8. 8. The use according to claim 7, wherein the product further comprises an adjuvant.
9. 9. The use according to claim 7, wherein the product is in a dosage form selected from one or more of solutions, injections, sprays, nasal drops, aerosols, powder sprays, tablets, capsules and granules.
10. 10. A method for improving silk quality is characterized by comprising the step of treating silkworms with FKBP12B genes or biological materials related to the FKBP12B genes.

Description

Application of FKBP12B in improving silkworm survival rate and silk quality Technical Field The invention relates to the field of biotechnology, in particular to application of FKBP12B in improving silkworm survival rate and silk quality. Background Silk has found wide application in textile industry, biomedical science and tissue engineering due to its excellent mechanical properties, biocompatibility and biodegradability. Natural Color Silkworms (NCSs) are particularly popular because they do not require chemical staining. These silks are produced by transferring pigments in mulberry leaves into silk glands by specific genes, and have similar structure and performance to ordinary white silk. The golden autumn X early day is a new silkworm variety cultivated by Zhejiang university, and the approval of Zhejiang province crop variety (approval number: zhe 201700 1) is obtained in 1 month 2017. Cocoons of the variety are naturally yellow, chemical dyeing is not needed, but the cocoon layer rate is relatively low. The economic value of silkworm breeding is represented by the weight ratio of cocoon shells and the quality of the cocoon shells. FKBP12 (FK 506-binding protein 12) is a member of the FKBPs family, has a molecular weight of 12kD, is widely distributed in vivo, and is involved in various physiological activities such as immunosuppression, ion channel, cell cycle regulation and the like. There are studies showing that down-regulation of FKBP12 can inhibit body temperature decline and death caused by tnfα (tumor necrosis factor α) -treated mice, suggesting that FKBP12 is involved in the modulation of tnfα -induced inflammatory responses. FKBP12 inhibits expression of mTOR in mammals after binding to rapamycin to form a complex, thereby affecting the inhibition of T cell proliferation by interleukin 2 (IL-2). After FKBP12.6 is dissociated from RyR, the release of Ca 2+ is greatly increased, and the regulation of calcium regulation signals is unstable, so that cardiovascular diseases, neurodegenerative diseases and other diseases are caused. FKBP12 plays an important role in mammals and is involved in various physiological activities of lepidopteran insects. It has been found that FKBP12 gene is expressed in the period of cotton bollworm larvae, the expression level in the older larvae and in the pre-pupae is higher, and the expression in diapause pupae is higher than that in non-diapause pupae, and it is presumed that FKBP12 participates in the maintenance of diapause of cotton bollworms. Research shows that FKBP12A and FKBP12B genes exist in the bodies of 5-year-old tussah larvae, and the two genes are expressed in fat, midgut, silk gland and other organs, wherein the expression level of the FKBP12A gene is obviously higher than that of the FKBP12B gene. Up to now, no document has reported the use of FKBP12B in silkworms. Disclosure of Invention In view of the above-described drawbacks of the prior art, an object of the present invention is to provide the use of FKBP12B for improving silkworm survival and/or silk quality. To achieve the above and other related objects, the present invention is achieved by the following technical means. The first aspect of the present invention protects the use of the FKBP12B gene or a biological material related thereto in at least one of: a1 Improving the survival rate of silkworms; A2 Improving cocoon layer yield of silkworms; a3 Improving silk quality; A4 Preparing a product for improving the survival rate of silkworms; A5 Preparing a product for improving the cocoon layer yield of silkworms; A6 Preparing a product for improving the quality of silk. In another aspect, the invention provides a method for improving silk quality, comprising treating silkworms with FKBP12B gene or biological materials related thereto. Compared with the prior art, the invention has the following beneficial effects: 1) The invention discovers that after FKBP12B is given to silkworms for the first time, the survival rate, the cocoon shell weight, the cocoon layer rate and the silk quality of the silkworms are improved. After the silkworm FKBP12B is given, the weight of cocoon shells is 21.96% higher than that of a control group, the cocoon layer rate is 2.5% higher than that of the control group, and the weight of silk fibers is 33.60% higher than that of the control group. These results indicate that FKBP12B has potential application value in improving silk quality. 2) The invention discovers for the first time that after FKBP12B is given to silkworms, the death rate of silkworms can be reduced, and the death rate is 36.89% lower than that of a control group. Drawings FIG. 1 shows the relative expression analysis of FKBP12B in silkworms after feeding a recombinant expression vector in example 2 of the present invention. Data are expressed as mean ± SD, n=4. WP: wild plasmid (empty plasmid), RP: recombinant expression vector. FIG. 2 shows mortality of silkworm larvae of the control group an