CN-122005871-A - Rare earth up-conversion nanoparticle carrier and preparation method and application thereof

Abstract

The invention relates to a rare earth up-conversion nano-particle carrier and a preparation method and application thereof, and belongs to the technical field of nano-drug carriers, the method is used for solving at least one of the problems that the existing nano-carrier cannot fully examine the dynamic distribution rule and biological distribution characteristic of the nano-carrier, and the existing carrier is poor in stability, poor in water solubility, low in drug loading capacity and the like. The rare earth up-conversion nanoparticle carrier is of a multi-layer core-shell structure, polymer chains on the surface of the rare earth up-conversion nanoparticle carrier obtained by the method can be fully stretched, on one hand, better steric hindrance is provided to improve the water dispersibility and stability of the rare earth up-conversion nanoparticle carrier, and on the other hand, mutual overlapping and trapping among the polymer chains adsorbed on the surface are reduced to provide more anchoring sites, so that high-efficiency loading of vaccines/medicines is realized.

Inventors

• WANG SHUFENG
• XU QIAN

Assignees

• 北京书峰科技有限责任公司

Dates

Publication Date

20260512

Application Date

20260119

Claims (10)

1. 1. The preparation method of the rare earth up-conversion nanoparticle carrier is characterized by comprising the following steps of: (1) Dispersing hydrophobic rare earth up-conversion nano particles in a nonpolar solvent to obtain a first mixture; (2) Dispersing an amphiphilic high molecular polymer in a polar solvent to obtain a second mixture; (3) And mixing the first mixture and the second mixture, and removing the nonpolar solvent to obtain the rare earth up-conversion nanoparticle carrier.
2. 2. The method of claim 1, wherein in the step (1), the hydrophobic rare earth up-conversion nanoparticle is a hydrophobic up-conversion nanoparticle capable of emitting light.
3. 3. The method of claim 1, wherein in step (1), the nonpolar solvent comprises cyclohexane and/or chloroform.
4. 4. The method for preparing a rare earth up-conversion nanoparticle carrier according to any one of claims 1 to 3, wherein in the step (1), the concentration of the hydrophobic rare earth up-conversion nanoparticle in the nonpolar solvent is 0.1mg/mL to 20mg/mL.
5. 5. The method for preparing rare earth up-conversion nanoparticle carrier according to any one of claims 1 to 3, wherein in the step (2), the amphiphilic polymer comprises one or more of DSPE-PEG 100~20000 , tween-80 and TPGS.
6. 6. The method of claim 1, wherein in step (2), the polar solvent comprises secondary water and physiological solution.
7. 7. The method for preparing a rare earth up-conversion nanoparticle carrier according to claim 1, wherein in the step (2), the concentration of the amphiphilic polymer in the polar solvent is 0.1mg/mL to 100mg/mL.
8. 8. The method for preparing a rare earth up-conversion nanoparticle carrier according to claim 1, wherein in the step (3), the volume ratio of the first mixture to the second mixture is 2:10-10:2; And/or, in the step (3), the nonpolar solvent is removed by rotary evaporation or reduced pressure suction.
9. 9. A rare earth upconverting nanoparticle support prepared by the method of any one of claims 1-8.
10. 10. Use of the rare earth up-conversion nanoparticle support of claim 9 in fluorescence imaging and/or drug loading.

Description

Rare earth up-conversion nanoparticle carrier and preparation method and application thereof Technical Field The invention relates to the technical field of nano drug carriers, in particular to a rare earth up-conversion nano particle carrier and a preparation method and application thereof. Background Malignant tumors have become a major factor affecting human health, with their morbidity and mortality rising year by year. Traditional treatments for malignant tumors include surgical excision, chemotherapy and radiation therapy. Among them, chemotherapy, alone or in combination with other therapies, has been used to treat various types of tumors. However, chemical drugs have poor water solubility, systemic distribution and lack of tumor targeting ability, and damage to normal tissues while killing tumor cells, resulting in poor therapeutic effects and serious toxic and side effects. The chemical drugs are loaded on the drug delivery carrier, so that the water solubility of the drug delivery carrier can be effectively improved, the bioavailability is improved, and the targeted transportation of cells and specific tissues is realized. Therefore, the anti-tumor efficiency of the chemical medicine can be obviously improved by utilizing the nano medicine carrier, and the nano medicine carrier has huge clinical application prospect in tumor treatment. In order to evaluate the protective ability of various vaccines and the anti-tumor ability of chemical drugs, it is necessary to develop an in vivo tracing and measuring technique, which is combined with nano-carriers in a certain way, so that the vaccines or chemical drugs can be observed on their biodistribution characteristics by using a certain instrument (such as a camera, a mobile phone, a microscope) after entering the body. The nanometer materials used for vaccine/chemical drug delivery in the prior art comprise phosphate nanometer materials, high molecular polymer nanometer materials, inorganic nanometer carriers and the like. Although fluorescent labeling techniques can be used to track the nanocarriers described above, the techniques are limited by the depth of tissue penetration. That is, the nanocarriers that penetrate deep into the tissue and organs of the body cannot be precisely positioned by the conventional fluorescent labeling technique. Disclosure of Invention In view of the above analysis, the present invention aims to provide a rare earth up-conversion nanoparticle carrier, a preparation method and an application thereof, so as to solve at least one of the problems of poor stability, poor water solubility, low drug loading capacity and the like of the existing nano carrier that the dynamic distribution rule and biological distribution characteristics of the nano carrier cannot be fully examined. In a first aspect, the present invention provides a method for preparing a rare earth up-conversion nanoparticle support, the method comprising the steps of: (1) Dispersing hydrophobic rare earth up-conversion nano particles in a nonpolar solvent to obtain a first mixture; (2) Dispersing an amphiphilic high molecular polymer in a polar solvent to obtain a second mixture; (3) And mixing the first mixture and the second mixture, and removing the nonpolar solvent to obtain the rare earth up-conversion nanoparticle carrier. Further, in the step (1), the hydrophobic rare earth up-conversion nanoparticle is a hydrophobic up-conversion nanoparticle capable of emitting light. Further, in step (1), the nonpolar solvent includes cyclohexane and/or chloroform. Further, in the step (1), the concentration of the hydrophobic rare earth up-conversion nano particles in the nonpolar solvent is 0.1 mg/mL-20 mg/mL. Further, in the step (2), the amphiphilic polymer comprises one or more of DSPE-PEG 100~20000, tween-80 and TPGS. Further, in the step (2), the polar solvent comprises secondary water and physiological solution. Further, in the step (2), the concentration of the amphiphilic polymer in the polar solvent is 0.1 mg/mL-100 mg/mL. Further, in the step (3), the volume ratio of the first mixture to the second mixture is 2:10-10:2; And/or, in the step (3), the nonpolar solvent is removed by rotary evaporation or reduced pressure suction. In a second aspect, the present invention provides a rare earth up-conversion nanoparticle support prepared by the above method. In a third aspect, the invention provides an application of the rare earth up-conversion nanoparticle carrier in fluorescence imaging and/or drug loading. Compared with the prior art, the invention has at least one of the following beneficial effects: (1) The rare earth up-conversion nanoparticle carrier is of a multi-layer core-shell structure, and macromolecule chains on the surface of the rare earth up-conversion nanoparticle carrier obtained by the method can be fully stretched, so that on one hand, better steric hindrance is provided to improve the water dispersibility and stability of the rare earth up-