CN-122005895-A - Biological dressing and preparation method and application thereof

Abstract

The invention provides a biological dressing, a preparation method and application thereof, wherein the preparation method comprises the steps of still water thawing, mao Tuozhi removing, cutting and dividing, finishing and punching, iodophor soaking, purified water soaking, protease and alkali metal hydroxide mixed solution soaking, purified water soaking, antibacterial brushing, packaging and irradiation. The product obtained by the invention can purify, clean and protect the wound surface, promote the growth of granulation tissues, cover the granulation wound surface, chronic ulcer and bedsore, shorten the healing time and improve the healing effect.

Inventors

• LI WENHONG
• WU ZILU
• GAO ZHEZHU
• LI JUN
• HU ZHIWEI
• Tie Xiaojun

Assignees

• 天津市托福医用原子能科技有限公司

Dates

Publication Date

20260512

Application Date

20260327

Claims (10)

1. 1. A method for preparing a biological dressing, the method comprising the steps of: (1) Soaking biological skin in water for the first time to obtain a semi-finished product A; (2) Scraping the semi-finished product A obtained in the step (1) to obtain a semi-finished product B; (3) Cutting the semi-finished product B obtained in the step (2) to obtain a semi-finished product C; (4) Punching the semi-finished product C obtained in the step (3) to obtain a semi-finished product D; (5) Soaking the semi-finished product D obtained in the step (4) by using an iodophor solution to obtain a semi-finished product E; (6) Soaking the semi-finished product E obtained in the step (5) with water for the second time to obtain a semi-finished product F; (7) Soaking the semi-finished product F obtained in the step (6) by using a solution of protease and alkali metal hydroxide to obtain a semi-finished product G; (8) Soaking the semi-finished product G in the step (7) with water for the third time to obtain a semi-finished product H; (9) Performing antibacterial treatment on the semi-finished product H obtained in the step (8) by using an antibacterial agent solution to obtain a semi-finished product I; (10) And (3) irradiating the semi-finished product I obtained in the step (9) by using Co 60 to obtain the biological dressing.
2. 2. The method of claim 1, wherein the biological skin in step (1) comprises pigskin or cow hide; preferably, the soaking time in the step (1) is 6-8 hours; Preferably, the soaking temperature in the step (1) is 10-15 ℃; preferably, in the step (1), the mass ratio of the biological skin to the water is 1 (5-10).
3. 3. The method of claim 1, wherein the concentration of the iodophor solution in step (5) is 50-200 mg/L.
4. 4. The preparation method of claim 1, wherein 1-3 ml of iodophor solution is used for soaking each square centimeter of semi-finished product D in the step (5); preferably, the soaking time is 5-15 min.
5. 5. The method according to claim 1, wherein 1 to 3ml of water is used per square centimeter of the semi-finished product E in the step (6); preferably, in the step (6), after soaking, shaking is performed, water is replaced, and shaking and water replacement are repeated until the pH value of the water is 5.5-6.5.
6. 6. The preparation method of claim 1, wherein the mass ratio of protease to alkali metal hydroxide in the step (7) is 5-15:1.
7. 7. The preparation method according to claim 1, wherein 1-3 ml of the solution is used for soaking each square centimeter of the semi-finished product F in the step (7); Preferably, the soaking time is 2-4 hours.
8. 8. The method of claim 1, wherein the antimicrobial agent in step (9) is a zinc sulfadiazine ointment; Preferably, the mass concentration of the antibacterial agent solution in the step (9) is 2.5-4 g/L.
9. 9. The biological dressing prepared by the preparation method according to any one of claims 1 to 8.
10. 10. Use of a biological dressing according to claim 9 for the preparation of a cover material for a wound bed.

Description

Biological dressing and preparation method and application thereof Technical Field The invention relates to the technical field of biological materials, in particular to a biological dressing, a preparation method and application thereof. Background The wound surface covering dressing applied clinically at present is mainly divided into three major categories of traditional dressing, synthetic dressing and natural biological dressing, wherein the biological skin-based biological dressing has unique advantages in wound repair due to the physiological characteristics similar to human skin, is classified as a III-type medical device and is mainly used for covering and repairing burns and scalds and deep wound surfaces. Natural biological dressing is the focus of research by virtue of excellent biocompatibility, wherein biological skin dressing (including autologous skin, allogenic skin, xenogenic skin, acellular dermal matrix and the like) is prominent in aspects of moisture permeability, adhesiveness, hemostasis, epithelialization and the like due to the simulation of barrier function and structural characteristics of human skin. Autologous skin grafting is an ideal scheme for wound repair, but for patients with large-area burn wounds, autologous skin sources are seriously insufficient, and clinical requirements are difficult to meet. The allogenic skin and xenogenic skin (such as irradiated pigskin) are used as alternative materials, can effectively prevent water and nutrition loss of the wound surface and resist bacterial invasion, but the clinical application is limited by multiple bottlenecks, namely, the allogenic skin and the xenogenic skin have strong antigenicity and are easy to cause immune rejection, usually dissolve and fall off within 2-3 weeks, the primary healing of the wound surface can not be realized, the existing treatment technology (such as freezing preservation and glycerol preservation) can reduce part of antigenicity, but has the problems of harsh preservation conditions, complex preparation flow, risk of pathogenic microorganism pollution and the like, and meanwhile, the biological skin dressing lacks the capability of dynamically regulating and controlling the healing of the wound surface, can not accurately release bioactive substances according to different stages of healing, and is difficult to realize the non-scar healing and skin function reconstruction. CN105079880B discloses a preparation method of a heterogeneous decellularized dermis matrix with good biocompatibility, which specifically comprises the following steps of (1) preparing a fault dermis sheet, (2) separating epidermis and dermis, (3) decellularizing treatment, (4) crosslinking, (5) virus inactivation, (6) refining and (7) Co-60 irradiation sterilization. The decellularized tissue engineering material obtained by the method has stable cytotoxicity and better cell compatibility. However, the materials (without the epidermis component) provided by this method have not achieved the effect of the dermis and epidermis structures. In summary, various drawbacks are common to current biological skin dressings. Therefore, the development of the biological skin dressing which has low antigenicity, excellent biocompatibility, stable mechanical property, can adapt to the requirements of different stages of wound healing, has simple preparation process and is easy for large-scale production becomes a technical problem to be solved in the field of wound repair. Disclosure of Invention The invention aims to solve the technical problems that the existing biological dressing has poor biocompatibility and mechanical effect. In order to solve the technical problems, in one aspect, the present invention provides a preparation method of a biological dressing, the preparation method comprising the following steps: (1) Soaking biological skin in water for the first time to obtain a semi-finished product A; (2) Scraping the semi-finished product A obtained in the step (1) to obtain a semi-finished product B; (3) Cutting the semi-finished product B obtained in the step (2) to obtain a semi-finished product C; (4) Punching the semi-finished product C obtained in the step (3) to obtain a semi-finished product D; (5) Soaking the semi-finished product D obtained in the step (4) by using an iodophor solution to obtain a semi-finished product E; (6) Soaking the semi-finished product E obtained in the step (5) with water for the second time to obtain a semi-finished product F; (7) Soaking the semi-finished product F obtained in the step (6) by using a solution of protease and alkali metal hydroxide to obtain a semi-finished product G; (8) Soaking the semi-finished product G in the step (7) with water for the third time to obtain a semi-finished product H; (9) Performing antibacterial treatment on the semi-finished product H obtained in the step (8) by using an antibacterial agent solution to obtain a semi-finished product I; (10) And (3) irradiati