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CN-122005896-A - Implant coating method based on sulfonated chitosan

CN122005896ACN 122005896 ACN122005896 ACN 122005896ACN-122005896-A

Abstract

The invention discloses an implant coating method based on sulfonated chitosan, which comprises the steps of mixing the sulfonated chitosan with hydrogel and coating the mixture on the surface of an implant, or coating the sulfonated chitosan on the implant after plasma surface treatment. According to the method, the sulfonated chitosan is firmly coated on the surfaces of the medical screw, the suture and the PET artificial ligament by using a plasma treatment or hydrogel wrapping method, so that the adhesive force and the stability of the sulfonated chitosan coating are obviously improved, the biological functionality of the sulfonated chitosan in tissue repair is fully exerted, and the compatibility and the repair effect of medical materials are improved. Specifically, the coating takes the bioactivity of sulfonated chitosan as a core, and realizes the functional enhancement of medical materials by promoting the polarization of M2 type macrophages, inhibiting inflammatory reaction, reducing scar formation and promoting angiogenesis and osteogenesis, and optimizes the compatibility and repair effect of the medical materials in biological environment.

Inventors

  • LIU CHANGSHENG
  • WANG SHUANG
  • WANG JING
  • CUI JINGYUAN

Assignees

  • 华东理工大学

Dates

Publication Date
20260512
Application Date
20241112

Claims (10)

  1. 1. The method for coating the implant based on the sulfonated chitosan is characterized by comprising the step of coating the sulfonated chitosan on the surface of the implant, wherein the sulfonated chitosan is mixed with hydrogel and then coated on the surface of the implant, and/or the implant is subjected to plasma surface treatment and then coated with the sulfonated chitosan.
  2. 2. The method of implant coating of claim 1, wherein the implant is a medical screw, a medical suture, or a medical artificial ligament.
  3. 3. The method of implant coating of claim 1, wherein the implant is made of polyethylene terephthalate, polyglycolic acid, polylactic acid, or polylactic acid-glycolic acid copolymer.
  4. 4. The implant coating method of claim 1, wherein the method comprises the steps of: (i) Uniformly mixing sulfonated chitosan and hydrogel prepolymer to prepare hydrogel composite material solution, wherein the hydrogel composite material solution is hydrogel prepolymer solution containing sulfonated chitosan; (ii) Immersing the implant in the hydrogel composite material solution prepared in the step i), so that the hydrogel composite material covers the surface of the implant to form a hydrogel layer; (iii) Solidifying the hydrogel layer to form a sulfonated chitosan coating on the surface of the implant.
  5. 5. The method of coating an implant according to claim 1, wherein in step i), the hydrogel composite solution is prepared by mixing methacryloylated gelatin, eight arm polyethylene glycol diacrylate, sulfonated chitosan, and phenyl-2, 4, 6-trimethylbenzopyridine phosphate in a phosphate buffered saline solution.
  6. 6. The implant coating method according to claim 1, wherein the plasma surface treatment is performed using a plasma treatment apparatus, and the surface of the implant is aminated with oxygen or nitrogen, thereby enhancing the reactivity of the surface of the implant.
  7. 7. The implant coating process of claim 6, wherein the plasma treatment device has a power of 70-100 watts and a treatment time of 5-10 minutes.
  8. 8. The method of coating an implant of claim 6, comprising immersing the plasma treated implant in a solution of sulfonated chitosan, saturation adsorption by the combination of amino groups and sulfonic acid groups, and coating the sulfonated chitosan.
  9. 9. A biomedical implant prepared by the implant coating method of any one of claims 1-8.
  10. 10. Use of a biomedical implant according to claim 9 for the preparation of medical sutures, catheters, screws, stents or ligament reconstruction materials.

Description

Implant coating method based on sulfonated chitosan Technical Field The invention relates to the technical field of surface modification of medical materials, in particular to an implant coating method based on sulfonated chitosan. Background The current artificial ligament and suture material has the problems of poor biocompatibility, strong inflammatory response, poor tissue integration effect and the like after operation, so that the patient has the adverse effects of slow recovery, obvious scars, poor bone tissue fixation effect and the like. Sulfonated Chitosan (SCS) as a material with multiple biological activities can promote polarization of M2 type macrophages, regulate inflammatory reaction, inhibit excessive scar tissue formation, effectively promote angiogenesis and osteogenesis, has excellent tissue repair ability, and how to uniformly and firmly attach a sulfonated chitosan coating to the surface of medical materials such as screws, sutures, PET artificial ligaments, etc., becomes a key to solve the above problems. However, the traditional physical or chemical coating method often causes uneven coating or insufficient adhesive force, which affects the long-term use effect. Thus, there is a need to further develop coating methods. Disclosure of Invention The invention aims to provide a coating method which enables SCS to be firmly coated on the surface of medical materials, thereby playing a core role in tissue repair and postoperative inflammation regulation. The method includes the following two coating techniques. In a first aspect of the invention, a sulfonated chitosan-based implant coating method is provided, the method comprises the step of coating sulfonated chitosan on the surface of an implant, wherein the sulfonated chitosan is mixed with hydrogel and then coated on the surface of the implant, and/or the implant is coated with sulfonated chitosan after plasma surface treatment. In the invention, the sulfonated chitosan material is uniformly mixed with the hydrogel, and the adhesion property of the hydrogel is utilized to be tightly combined with the surface of an implant material (such as PET material, PGA material and PLA/PLGA material). In the invention, the surface of an implant material (such as PET material, PGA material and PLA/PLGA material) is subjected to amino treatment by plasma equipment, so that the implant material is saturated and adsorbed in a sulfonated chitosan solution, and the adhesive force of a coating is improved by the combination of amino and sulfonic groups. The biological functionality of the SCS coating realizes the following repairing functions on the surface of the medical material, such as promoting angiogenesis, regulating and controlling inflammatory reaction, relieving scar formation and accelerating tissue repairing process, thereby remarkably improving the biocompatibility and repairing effect of the medical material. In another preferred embodiment, the implant is a medical screw, a medical suture or a medical artificial ligament. The sulfonated chitosan coating can provide excellent adhesive force and biocompatibility, and remarkably improve the tissue repair effect. In another preferred embodiment, the implant is made of polyethylene terephthalate (PET), polyglycolic acid (PGA), polylactic acid PLA, polylactic-co-glycolic acid (PLGA). In another preferred embodiment, the artificial ligament is made of polyethylene terephthalate (PET), the suture is made of polyglycolic acid (PGA), and the screw is made of polylactic acid or polylactic-co-glycolic acid (PLA or PLGA). In another preferred embodiment, the method comprises the steps of: (i) Uniformly mixing sulfonated chitosan and hydrogel prepolymer to prepare hydrogel composite material solution, wherein the hydrogel composite material solution is hydrogel prepolymer solution containing sulfonated chitosan; (ii) Immersing the implant in the hydrogel composite material solution prepared in the step i), so that the hydrogel composite material covers the surface of the implant to form a hydrogel layer; (iii) Solidifying the hydrogel layer to form a sulfonated chitosan coating on the surface of the implant. In another preferred embodiment, the concentration of sulfonated chitosan used in step i) is 0.1% (w/v) to 1.0% (w/v), preferably 0.2% (w/v) to 0.8% (w/v), more preferably 0.4% (w/v), 0.5% (w/v) or 0.6% (w/v). Wherein w/v is g/ml. In another preferred embodiment, the implant is immersed in the hydrogel composite material after a pretreatment, which is a cleaning and drying process of the surface. In another preferred embodiment, the hydrogel layer has a porous structure to provide excellent lubricity and nutrient transfer capability. In another preferred embodiment, the hydrogel composite is cured by heating or solvent treatment. In another preferred example, in the step i), the hydrogel composite solution is prepared by mixing methacryloylated gelatin, eight-arm polyethylene glycol diacrylate, sulf