CN-122005900-A - Wet tissue adhesive and preparation method and application thereof

Abstract

The invention relates to the technical field of biomedical materials, in particular to a wet tissue adhesive, a preparation method and application thereof, which comprises hydrogel formed by cationic polysaccharide, anionic polysaccharide, calcium-based powder and polyphosphoric acid organic acid through double decomposition reaction, wherein the calcium-based powder reacts with the polyphosphoric acid organic acid to generate carbon dioxide bubbles which damage an interface hydration layer and nano calcium polyphosphate which is crosslinked in situ to enhance cohesion. The invention realizes synchronous enhancement of mechanical property and adhesive property by means of high-efficiency water-repellent strong adhesion and nano-crosslinking cohesion retention of carbon dioxide bubbles generated in situ, has good biocompatibility and degradability, and can effectively solve the problem of tissue adhesion in a humid environment.

Inventors

• FAN XIANMOU
• ZHANG BING
• LI CHUNMEI
• QIU XIAOFEN
• YANG TENG
• XU WEN

Assignees

• 广东医科大学附属医院

Dates

Publication Date

20260512

Application Date

20260415

Claims (10)

1. 1. A wet tissue adhesive is characterized in that the wet tissue adhesive is hydrogel and is formed by double decomposition reaction of cationic polysaccharide, anionic polysaccharide, calcium-based powder and polyphosphoric acid organic acid, wherein the calcium-based powder reacts with the polyphosphoric acid organic acid to generate in situ carbon dioxide bubbles and nano-crosslinked nano-calcium polyphosphate which damage an interface hydration layer.
2. 2. The wet tissue adhesive according to claim 1, wherein the solid-to-liquid ratio of the cationic polysaccharide, the anionic polysaccharide, the calcium-based powder and the polyphosphoric acid organic acid is 0.10-0.90g:0.10-0.20g:4.00mL, and the sum of the mass of the cationic polysaccharide and the mass of the anionic polysaccharide is 1.00g.
3. 3. The wet tissue adhesive of claim 1 wherein the cationic polysaccharide comprises one of quaternized chitosan, hydroxybutyl chitosan, and hydroxypropyl chitosan.
4. 4. The wet tissue adhesive according to claim 1, wherein the anionic polysaccharide comprises one of sodium alginate, sodium carboxymethylcellulose, and hyaluronic acid.
5. 5. The wet tissue adhesive according to claim 1, wherein the calcium-based powder comprises one of eggshell powder and calcium carbonate.
6. 6. The wet tissue adhesive of claim 1 wherein the polyphosphoric acid organic acid comprises one of phytic acid, pyrophosphoric acid, triphosphoric acid, and polyphosphoric acid.
7. 7. The wet tissue adhesive according to claim 6, wherein the polyphosphoric acid organic acid is an aqueous solution of polyphosphoric acid organic acid having a mass fraction of 5 to 40%.
8. 8. A method for preparing a wet tissue adhesive according to any one of claims 1 to 7, comprising the steps of: S1, preparing mixed powder, namely adding cationic polysaccharide, anionic polysaccharide and calcium-based powder into an agate mortar at room temperature, and grinding for 8-12min to obtain uniformly dispersed mixed powder; S2, in-situ gel forming reaction, namely transferring the mixed powder of the S1 into a beaker, adding an organic acid aqueous solution of polyphosphoric acid, stirring for 5-10min by using a glass rod, and forming uniform hydrogel after double decomposition reaction, thus obtaining the wet tissue adhesive.
9. 9. The method of claim 8, wherein in the step of S2 double decomposition, calcium salt in the calcium-based powder reacts with phosphate groups of the polyphosphoric acid organic acid to generate carbon dioxide bubbles and nano calcium polyphosphate in situ.
10. 10. The use of a wet tissue adhesive according to any one of claims 1 to 7 for the preparation of a medical dressing.

Description

Wet tissue adhesive and preparation method and application thereof Technical Field The invention relates to the technical field of biomedical materials, in particular to a wet tissue adhesive and a preparation method and application thereof. Background In recent years, tissue adhesive capable of adhering to moist tissues has become a breakthrough tool in the field of tissue regeneration, and has multiple functions of wound closure, physical protection, antibiosis, microenvironment regulation, targeted drug delivery and the like. However, wetting the interfacial aqueous layer that persists on the tissue surface can seriously affect the utility of the bioadhesive, for example, water molecules can form competitive barrier membranes with the adhesive or tissue functional groups, hinder the effective action between interfaces, affect the wetting and adhesion capabilities of the tissue adhesive glue, and ultimately lead to the problems of poor wound closure effect, insufficient physical protection, and the like. In order to solve the above problems, there have been studies on achieving adhesion by absorbing interfacial water and forming an adhesive hydrogel in situ using xerogel, powder and film-like materials. For example, yuk et al dehydrate hydrogels containing carboxylic acid and N-hydroxysuccinimide ester groups to form dry double sided adhesives, which absorb interfacial water and swell to bring it into intimate contact with tissue, and by this contact form an effective adhesion, resulting in strong adhesion (DOI: 10.1038/s 41586-019-1710-5). In addition, self-gelling polyethylenimine/polyacrylic acid powder can also form hydrogel in situ by absorbing interfacial water, and can firstly realize close contact with tissues, and then jointly achieve strong adhesion by virtue of effective adhesion effect and diffusion of polymer to a substrate network (DOI: 10.1126/Sciadv.abe8739). However, the effect of removing interfacial water by the method is limited, and excessive water absorption can damage the network structure and mechanical property of the adhesive hydrogel, which can lead to the reduction of the adhesive property. Disclosure of Invention The invention aims to provide a wet tissue adhesive, a preparation method and application thereof, and the wet tissue adhesive is used for realizing high-efficiency water-repellent strong adhesion and nano-crosslinking cohesion retention of an interface by means of carbon dioxide bubbles and nano calcium polyphosphate generated in situ, synchronously enhancing mechanical properties and adhesion properties, has good biocompatibility and degradability, and can effectively solve the problem of tissue adhesion in a wet environment. In order to achieve the above purpose, the present invention provides a wet tissue adhesive, which is a hydrogel, and is formed by a double decomposition reaction of a cationic polysaccharide, an anionic polysaccharide, a calcium-based powder and a polyphosphoric acid organic acid, wherein the calcium-based powder reacts with the polyphosphoric acid organic acid to generate in situ carbon dioxide bubbles and nano-crosslinked nano calcium polyphosphate, which damage an interface hydration layer. Preferably, the solid-to-liquid ratio of the cationic polysaccharide, the anionic polysaccharide, the calcium-based powder and the polyphosphoric acid organic acid is 0.10-0.90g:0.10-0.20g:4.00mL, and the sum of the mass of the cationic polysaccharide and the mass of the anionic polysaccharide is 1.00g. Preferably, the cationic polysaccharide comprises one of quaternized chitosan, hydroxybutyl chitosan, hydroxypropyl chitosan. Preferably, the anionic polysaccharide comprises one of sodium alginate, sodium carboxymethylcellulose and hyaluronic acid. Preferably, the calcium-based powder comprises one of eggshell powder and calcium carbonate. Preferably, the polyphosphoric acid organic acid includes one of phytic acid, pyrophosphoric acid, triphosphoric acid, and polyphosphoric acid. Preferably, the polyphosphoric acid organic acid is an aqueous solution of polyphosphoric acid organic acid having a mass fraction of 5 to 40%. The preparation method of the wet tissue adhesive comprises the following steps: S1, preparing mixed powder, namely adding cationic polysaccharide, anionic polysaccharide and calcium-based powder into an agate mortar at room temperature, and grinding for 8-12min to obtain uniformly dispersed mixed powder; S2, in-situ gel forming reaction, namely transferring the mixed powder of the S1 into a beaker, adding an organic acid aqueous solution of polyphosphoric acid, stirring by using a glass rod for 5-10 min, and forming uniform hydrogel after double decomposition reaction, thus obtaining the wet tissue adhesive. Preferably, in the double decomposition reaction process in S2, the calcium salt in the calcium-based powder reacts with the phosphate group of the polyphosphoric acid organic acid to generate carbon dioxide bubbles and nano calciu