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CN-122005906-A - Hydrogel dressing for promoting healing of diabetic wound surface as well as preparation method and application thereof

CN122005906ACN 122005906 ACN122005906 ACN 122005906ACN-122005906-A

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a hydrogel dressing for promoting healing of diabetic wounds, and a preparation method and application thereof. The hydrogel dressing for promoting the healing of the diabetic wound surface is prepared by taking glycyrrhizic acid and S-allyl-L-cysteine as raw materials and buffer solution as a dispersion medium, and by controlling the mass ratio of the raw materials, the reaction temperature, the reaction time and the pH, the glycyrrhizic acid and the S-allyl-L-cysteine are subjected to compound self-assembly. The hydrogel provided by the invention has the viscoelastic characteristics of similar tissues, and can be adaptively deformed along with the movement of a wound, so that continuous protection is provided for a chronic wound under a dynamic physiological condition, and the hydrogel has the flexibility of adapting to dynamic requirements of different healing stages.

Inventors

  • ZHU WENTING
  • YAN PENGKE
  • WANG YUSHAN
  • YUAN ZHONGWEN
  • Kuang Ningjie

Assignees

  • 广州医科大学附属第三医院（广州重症孕产妇救治中心、广州柔济医院）

Dates

Publication Date
20260512
Application Date
20260330

Claims (10)

  1. 1. The hydrogel dressing for promoting the healing of the diabetic wound is characterized by being prepared by taking glycyrrhizic acid and S-allyl-L-cysteine as raw materials and buffer solution as a dispersion medium, and compounding and self-assembling the glycyrrhizic acid and the S-allyl-L-cysteine by controlling the mass ratio of the raw materials, the reaction temperature, the reaction time and the pH.
  2. 2. The hydrogel dressing for promoting healing of a diabetic wound according to claim 1, wherein the buffer solution is a phosphate buffer solution.
  3. 3. The hydrogel dressing for promoting the healing of a diabetic wound according to claim 2, wherein the buffer solution is a 0.1 x phosphate buffer solution with a pH of 7-8.
  4. 4. The hydrogel dressing for promoting the healing of diabetic wounds according to claim 1, wherein the mass ratio of glycyrrhizic acid to S-allyl-L-cysteine is 10:2-8.
  5. 5. The method for preparing a hydrogel dressing for promoting the healing of a diabetic wound according to any one of claims 1 to 4, comprising the steps of: s1, glycyrrhizic acid is taken, buffer solution is added, vortex ultrasonic treatment is carried out until complete dissolution is carried out, and a clear and transparent glycyrrhizic acid solution is obtained; s2, taking S-allyl-L-cysteine, adding buffer solution, and performing vortex ultrasonic treatment until the S-allyl-L-cysteine is completely dissolved to obtain clear and transparent S-allyl-L-cysteine solution; And S3, placing the glycyrrhizic acid solution obtained in the step S1 in a water bath environment, continuously stirring, adding the S-allyl-L-cysteine solution obtained in the step S2, continuously stirring until the pH value of the system is stabilized at 7-8, and transferring the system to a refrigerator to form the hydrogel dressing.
  6. 6. The method for preparing a hydrogel dressing for promoting healing of a diabetic wound according to claim 5, wherein the concentration of the glycyrrhizic acid solution in the step S1 is 10-20 mg/mL.
  7. 7. The method for preparing a hydrogel dressing for promoting healing of a diabetic wound according to claim 5, wherein the concentration of the S-allyl-L-cysteine solution in the step S2 is 2-8 mg/mL.
  8. 8. The method for preparing the hydrogel dressing for promoting the healing of the diabetic wound surface according to claim 5, wherein the ultrasonic power in the step S1 is 200-300W, the ultrasonic time is 1-10 minutes, the ultrasonic power in the step S2 is 100-300W, and the ultrasonic time is 1-10 minutes.
  9. 9. The method for preparing the hydrogel dressing for promoting the healing of the diabetic wound surface according to claim 5, wherein the temperature of the water bath environment in the step S3 is 60-80 ℃, the continuous stirring time is 10-60 minutes, and the continuous stirring time is 10-60 minutes.
  10. 10. Use of a hydrogel dressing for promoting healing of a diabetic wound according to any one of claims 1 to 4 or a hydrogel dressing prepared by a method for preparing a hydrogel dressing for promoting healing of a diabetic wound according to any one of claims 5 to 9 in the preparation of a medicament for promoting healing of a diabetic wound.

Description

Hydrogel dressing for promoting healing of diabetic wound surface as well as preparation method and application thereof Technical Field The invention belongs to the technical field of medicines, and particularly relates to a hydrogel dressing for promoting healing of diabetic wounds, and a preparation method and application thereof. Background Chronic wounds (such as foot ulcers) of diabetes are often in an inflammatory phase arrest state and extremely difficult to heal due to high-sugar microenvironment, persistent inflammation and severe hypoxia. Although modern wound dressings (such as hydrogels, hydrocolloids and polymer films) can provide physical protection and moisture retention, for example, chinese patent application CN106267302a discloses an intelligent moisture management hydrogel composite dressing, which comprises a substrate layer and a hydrogel layer covering the surface of the substrate layer, wherein the hydrogel layer undergoes sol-gel transition, the whole hydrogel layer contains 40-80% of water, the surface becomes a hydrophobic interface, and the surface is obtained after being combined with a water-absorbent non-woven fabric substrate, but the adaptability of the dressing to dynamic wound microenvironment is limited, which often results in poor healing effect, especially in full-thickness skin injury. Studies have shown that by dual strategies of reducing ROS levels and directing polarization of M2 macrophages, it is expected to inhibit inflammation, stimulate angiogenesis, and restore efficient wound regeneration. In recent years, self-assembled hydrogel systems constructed based on natural drug small molecules have become the leading edge of research in the biomedical field. Compared with the traditional hydrogel relying on inert polymers, the system has the core advantage of realizing the design concept of carrier-drug integration, namely, natural drug molecules are not only used as structural frameworks for constructing a gel three-dimensional network, but also retain and exert the endogenous pharmacological activity of the natural drug molecules, so that the introduction of a large amount of exogenous auxiliary materials is avoided, and the biological safety and the treatment efficiency are remarkably improved. Hydrogen sulfide (H 2 S) as a key gas signaling molecule has shown great potential in regulating inflammation, oxidative stress, and extracellular matrix remodeling, a process critical to effective wound healing. Cystathionine-gamma-lyase (CSE) is a core enzyme for its synthesis. However, conventional H 2 S delivery systems lack the flexibility to accommodate dynamic demands of different healing stages, thereby limiting their therapeutic effectiveness. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide a hydrogel dressing for promoting the healing of diabetic wounds, and a preparation method and application thereof. According to the invention, through intermolecular interaction of glycyrrhizic acid and S-allyl-L-cysteine, a novel pharmaceutically active molecular hydrogel is constructed, physical encapsulation and chemical stability of SAC are realized by utilizing a three-dimensional network structure of GA, and an intelligent bioactive platform capable of responding to wound surface requirements is constructed through synergistic effect of the two. The hydrogel provided by the invention has the viscoelastic characteristics of similar tissues, and can be adaptively deformed along with the movement of a wound, so that continuous protection is provided for a chronic wound under a dynamic physiological condition, and the hydrogel has the flexibility of adapting to dynamic requirements of different healing stages. The technical scheme of the invention is as follows: The hydrogel dressing is prepared by taking glycyrrhizic acid and S-allyl-L-cysteine as raw materials and buffer solution as a dispersion medium, and by controlling the mass ratio of the raw materials, the reaction temperature, the reaction time and the pH, the glycyrrhizic acid and the S-allyl-L-cysteine are subjected to compound self-assembly. Further, the buffer solution is phosphate buffer solution. Further, the buffer solution is a 0.1 Xphosphate buffer solution with a pH of 7-8. Further, the buffer is a 0.1 x phosphate buffer solution with ph=7.4, which not only provides a mild, stable, near physiological pH dissolution environment, but also neutralizes the acidic pathological environment of the diabetic wound. Further, the mass ratio of glycyrrhizic acid to S-allyl-L-cysteine is 10:2-8. Further, the mass ratio of glycyrrhizic acid to S-allyl-L-cysteine is 10:2. The invention also provides a preparation method of the hydrogel dressing for promoting the healing of the diabetic wound, which comprises the following steps: s1, glycyrrhizic acid is taken, buffer solution is added, vortex ultrasonic treatment is carried out until complete dissolution is carried out, and a