CN-122005907-A - Enzyme polymer composite composition and application thereof

Abstract

The invention provides an enzyme polymer composite composition and application thereof, and relates to the technical field of medical materials. The enzyme polymer composite composition consists of hydrogen peroxide scavenging enzyme/SOD composite and dispersion liquid, the hydrogen peroxide scavenging enzyme/SOD composite is prepared through polymerization reaction, dialysis and drying of raw material components containing hydrogen peroxide scavenging enzyme, SOD, chitosan containing carbon-carbon double bond and its derivative and water-soluble or acid-soluble polymerizable monomer, and the dispersion liquid is selected from water or hydrogel. The enzyme-polymer composite composition of the invention can be used as a material for wound recovery and healing.

Inventors

• ZENG JIANGHUAI
• XU YUEHUA
• LAN ZHIYUAN

Assignees

• 厦门瑞聚医学科技有限公司

Dates

Publication Date

20260512

Application Date

20260408

Claims (11)

1. 1. An enzyme-polymer composite composition, which is characterized by comprising a hydrogen peroxide scavenging enzyme/SOD complex and a dispersion liquid; the hydrogen peroxide scavenging enzyme/SOD complex is obtained by carrying out polymerization reaction, dialysis and drying on raw material components containing hydrogen peroxide scavenging enzyme, SOD, chitosan containing carbon-carbon double bond and derivatives thereof and water-soluble or acid-soluble polymerizable monomers; The dispersion comprises water or a hydrogel; the weight ratio of the hydrogen peroxide scavenging enzyme/SOD complex to the dispersion is 1-20:100.
2. 2. The enzyme-polymer composite composition according to claim 1, wherein the chitosan containing a carbon-carbon double bond and its derivative are at least one of a maleamidated compound, an acrylated compound, and a maleated compound of chitosan and its derivative.
3. 3. The enzyme polymer composite composition according to claim 1, wherein the water-soluble or acid-soluble polymerizable monomer is composed of a monofunctional polymerizable monomer and a polyfunctional polymerizable monomer; The monofunctional polymerizable monomer comprises one or a combination of more than two of an amino group-containing or quaternary ammonium salt-containing polymerizable monomer, an acrylamide monomer, a hydroxyalkyl acrylate, (meth) acrylic acid and salts thereof, vinyl benzoic acid and salts thereof, and polyethylene glycol monoacrylate.
4. 4. The enzyme polymer composite composition according to claim 3, wherein the polyfunctional polymerizable monomer is one or a combination of two or more selected from the group consisting of a diacrylamide monomer and a polyethylene glycol diacrylate.
5. 5. The enzyme polymer composite composition according to claim 3, wherein the weight ratio of the monofunctional polymerizable monomer to the polyfunctional polymerizable monomer is 10 to 100:1.
6. 6. The enzyme-polymer complex composition according to claim 1, wherein the weight ratio of the hydrogen peroxide scavenging enzyme, the SOD, the chitosan containing carbon-carbon double bonds and derivatives thereof, and the polymerizable monomer is 10-0.5:1:0.1-5:10-120.
7. 7. The enzyme-polymer composite composition according to any one of claims 1 to 6, wherein the hydrogen peroxide scavenging enzyme is at least one selected from the group consisting of catalase, peroxidase, peroxide reductase, thioredoxin peroxidase, acetaldehyde dehydrogenase, superoxide dismutase, glutathione peroxidase, lipid peroxidase, cytoplasmin and collagenase.
8. 8. The enzyme-polymer composite composition according to claim 1, wherein the hydrogel is a temperature-sensitive hydrogel; the hydrogel has a phase transition temperature of 10-41 ℃.
9. 9. The enzyme-polymer composite composition according to claim 8, wherein the polymer material of the hydrogel is one or a combination of two or more selected from PEG-PPG、PEG-PPG-PEG、PCL-PEG-PCL、PCL-PEG、PEG-PLGA、PCL-PEG-PLGA、PLGA-PEG-PLGA、PNIPAAm-PEG、PNIPAAm-PEG-PPG、PNIPAAm、PNIPAAm-PVA、PDLLA-PEG-PDLLA and PNIPAAm-PAA.
10. 10. The enzyme-polymer complex composition according to claim 1, wherein the dispersion further comprises hyaluronic acid having a molecular weight of not more than 100 kDa; The weight percentage of the hyaluronic acid in the dispersion liquid is 0.1-1%.
11. 11. Use of the enzyme-polymer composite composition according to any one of claims 1 to 10 as a material for wound treatment.

Description

Enzyme polymer composite composition and application thereof Technical Field The invention belongs to the technical field of medical materials, and relates to an enzyme-polymer composite composition and application thereof. Background In the early stage of wound occurrence, immune cells such as macrophages engulf pathogens and clean necrotic tissues, but simultaneously generate a large amount of superoxide anion free radicals, which not only attack the invaded pathogens, but also seriously damage healthy cells and tissues around the wound, so that symptoms such as red swelling and pain appear around the wound, and the healing process of the wound is delayed. Superoxide dismutase SOD can effectively catalyze the conversion of superoxide anion free radicals into hydrogen peroxide, chinese patent CN116173283A discloses a chitosan-polyvinyl alcohol SOD enzyme hydrogel dressing, and the hydrogel layer consists of chitosan-polyvinyl alcohol hydrogel, SOD enzyme solution, gelatin and agar, and has the advantages of good biocompatibility, good antiallergic property and quick wound healing. The applicant believes that there is a need for further improvements and optimisation of the above techniques. Disclosure of Invention Superoxide anion radicals can be converted to hydrogen peroxide and oxygen under the action of SOD, but higher concentrations of hydrogen peroxide remain detrimental to wound healing. Hydrogen oxide can be effectively removed by hydrogen peroxide scavenging enzyme represented by catalase CAT, and the wound healing agent is beneficial to wound healing. Based on the above, the invention provides an enzyme polymer composite composition and application thereof. The technical scheme of the invention is as follows: An enzyme-polymer composite composition comprises hydrogen peroxide scavenging enzyme/SOD complex and dispersion; the hydrogen peroxide scavenging enzyme/SOD complex is obtained by carrying out polymerization reaction, dialysis and drying on raw material components containing hydrogen peroxide scavenging enzyme, SOD, chitosan containing carbon-carbon double bond and derivatives thereof and water-soluble or acid-soluble polymerizable monomers; The dispersion comprises water or a hydrogel; the weight ratio of the hydrogen peroxide scavenging enzyme/SOD complex to the dispersion is 1-20:100. Preferably, the chitosan containing carbon-carbon double bond and its derivative are at least one of maleated compound, acrylated compound and maleated compound of chitosan and its derivative. Preferably, the water-soluble or acid-soluble polymerizable monomer is composed of a monofunctional polymerizable monomer and a polyfunctional polymerizable monomer; The monofunctional polymerizable monomer comprises one or a combination of more than two of an amino group-containing or quaternary ammonium salt-containing polymerizable monomer, an acrylamide monomer, a hydroxyalkyl acrylate, (meth) acrylic acid and salts thereof, vinyl benzoic acid and salts thereof, and polyethylene glycol monoacrylate. More preferably, the polyfunctional polymerizable monomer is selected from one or a combination of two or more of a diacrylamide monomer and a polyethylene glycol diacrylate. More preferably, the weight ratio of the monofunctional polymerizable monomer to the polyfunctional polymerizable monomer is 10 to 100:1. Preferably, the weight ratio of the hydrogen peroxide scavenging enzyme, the SOD, the chitosan containing carbon-carbon double bonds and derivatives thereof and the polymerizable monomer is 10-0.5:1:0.1-5:10-120. Preferably, the hydrogen peroxide scavenging enzyme is at least one selected from the group consisting of catalase, peroxidase, peroxiredoxin, thioredoxin peroxidase, acetaldehyde dehydrogenase, superoxide dismutase, glutathione peroxidase, lipid peroxidase, cytoplasmin, and collagenase. Preferably, the hydrogel is a temperature-sensitive hydrogel, and preferably, the hydrogel has a phase transition temperature of 10-41 ℃. More preferably, the polymer material of the hydrogel is selected from one or a combination of two or more of PEG-PPG、PEG-PPG-PEG、PCL-PEG-PCL、PCL-PEG、PEG-PLGA、PCL-PEG-PLGA、PLGA-PEG-PLGA、PNIPAAm-PEG、PNIPAAm-PEG-PPG、PNIPAAm、PNIPAAm-PVA、PDLLA-PEG-PDLLA and PNIPAAm-PAA. Preferably, the dispersion further comprises hyaluronic acid having a molecular weight of not more than 100 kDa; The weight percentage of the hyaluronic acid in the dispersion liquid is 0.1-1%. Use of the enzyme-polymer composite composition according to any one of the embodiments described above as a material for wound treatment. The beneficial effects of the invention are as follows: (1) The enzyme-polymer composite composition comprises SOD and hydrogen peroxide scavenging enzyme, the SOD can convert superoxide anion free radicals into hydrogen peroxide and oxygen, the hydrogen peroxide scavenging enzyme converts and removes the hydrogen peroxide, so that not only is the superoxide anion free radicals removed, but also a