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CN-122005908-A - Anti-inflammatory gel for diabetic foot wound surface and preparation method thereof

CN122005908ACN 122005908 ACN122005908 ACN 122005908ACN-122005908-A

Abstract

The invention belongs to the field of biomedical dressings, and in particular relates to an anti-inflammatory gel for diabetic foot wound surfaces and a preparation method thereof. The composition comprises the following raw materials of sodium hyaluronate, naIO 4 , carboxymethyl chitosan, tannic acid, boric acid compounds, dexamethasone, dopamine hydrochloride, sodium citrate, PBS buffer solution and Tris buffer solution. The anti-inflammatory gel for the diabetic foot wound surface is based on an in-situ gel-forming dynamic cross-linked network, maintains a wet healing microenvironment, reduces dressing change traction injury, and realizes stable wet adhesion by introducing polydopamine, and balances fixity and dressing change friendliness. Adopts mild ion competition to control bacteria and rupture membranes, drug-loaded particles target anti-inflammatory and cooperate with tannic acid, and solves the inherent conflicts such as irritation, drug change injury and the like of the traditional dressing through multifunctional coupling design.

Inventors

  • Tan Qingying
  • XU YAO
  • WANG JIAWEI
  • YAO JIAQI
  • WANG XIUJING

Assignees

  • 中国人民解放军联勤保障部队第九〇三医院

Dates

Publication Date
20260512
Application Date
20260413

Claims (10)

  1. 1. The anti-inflammatory gel for the diabetic foot wound surface is characterized by comprising the following raw materials of sodium hyaluronate, carboxymethyl chitosan, tannic acid, boric acid compounds, dexamethasone, dopamine hydrochloride, sodium citrate, sodium gluconate, PBS buffer solution and Tris buffer solution.
  2. 2. The diabetic foot wound anti-inflammatory gel according to claim 1, wherein the molecular weight of the sodium hyaluronate ranges from 200 kda to 500kda.
  3. 3. The diabetic foot wound anti-inflammatory gel according to claim 1, wherein the boric acid compound is any one of borax, phenylboronic acid and 4-carboxyphenylboronic acid.
  4. 4. A method for preparing an anti-inflammatory gel for diabetic foot wound according to any one of claims 1 to 3, comprising the steps of: S1, dissolving sodium hyaluronate in deionized water, stirring until the sodium hyaluronate is completely dissolved to prepare an aqueous solution, then adding NaIO 4 in batches under the condition of avoiding light, adding ethylene glycol to terminate the reaction after the reaction is completed, purifying and dialyzing, pre-freezing, and performing vacuum freeze-drying to obtain oxidized modified sodium hyaluronate; S2, dissolving carboxymethyl chitosan in PBS buffer solution, and stirring until the solution is clear and uniform to prepare chitosan solution; S3, dissolving tannic acid in deionized water, stirring until the tannic acid is completely dissolved to prepare a TA solution, adding dexamethasone into the TA solution, stirring and dispersing uniformly, then dripping boric acid compound solution, regulating the pH of the system by using Tris buffer solution, continuing stirring, standing for aging, centrifuging, and taking the supernatant to obtain a TA-boric acid dynamic network drug-loaded particle dispersion; S4, adding dopamine hydrochloride into the chitosan solution in the step S2, regulating the pH of the system by using Tris buffer solution, stirring at room temperature for reaction, then adding the TA-boric acid dynamic network drug-carrying particle dispersion liquid in the step S3, uniformly dispersing by ultrasonic, and regulating the pH of the system after the dispersion is completed to obtain liquid B; s5, dissolving the oxidized modified sodium hyaluronate in the step S1 in PBS buffer solution, uniformly stirring and dissolving to prepare solution, then adding sodium citrate and sodium gluconate, and stirring until the solution is completely dissolved to obtain solution A; And S6, mixing the solution B in the step S4 and the solution A in the step S5 in proportion through a static mixing head, coating on a PTFE mold, and placing the PTFE mold in a relative moist environment for in-situ gel formation to obtain the anti-inflammatory gel.
  5. 5. The method for preparing the diabetic foot wound anti-inflammatory gel according to claim 4, wherein in the step S1, the mass usage ratio of NaIO 4 to sodium hyaluronate is 0.15-0.25:1, and the usage ratio of ethylene glycol to NaIO 4 is 0.4-0.8 mL:1g.
  6. 6. The method for preparing the anti-inflammatory gel for diabetic foot wound according to claim 4, wherein in the step S1, water is changed every 2-4 hours 3 times before purification dialysis, and then water is changed every 6-8 hours, and the total dialysis is performed for 48-72 hours.
  7. 7. The method for preparing the anti-inflammatory gel for diabetic foot wound according to claim 4, wherein in the step S2, the pH of the chitosan solution is 7.2-7.4, and the pH is adjusted by using PBS buffer solution.
  8. 8. A preparation method of the diabetic foot wound anti-inflammatory gel according to claim 4 is characterized in that in the step S3, the mass dosage ratio of dexamethasone to tannic acid is 0.1-0.15:1, the boric acid compound solution is prepared by dissolving boric acid compound in deionized water and adding Tris buffer solution for auxiliary dissolution, and the volume dosage ratio of the boric acid compound solution to TA solution is 0.1-0.3:1.
  9. 9. The method for preparing the anti-inflammatory gel for diabetic foot wound surface according to claim 4, wherein in the step S4, the adding amount of the TA-boric acid dynamic network drug-carrying particle dispersion liquid is 3-5% of the volume of the chitosan solution.
  10. 10. The method for preparing the diabetic foot wound anti-inflammatory gel according to claim 4, wherein in the step S5, the molar ratio of the sodium citrate to the sodium gluconate is 1-2:1.

Description

Anti-inflammatory gel for diabetic foot wound surface and preparation method thereof Technical Field The invention belongs to the field of biomedical dressings, and in particular relates to an anti-inflammatory gel for diabetic foot wound surfaces and a preparation method thereof. Background Diabetic foot ulcers are one of the common and serious chronic complications of diabetes, and their occurrence and development are usually associated with peripheral neuropathy, peripheral vascular disease, and immune and metabolic abnormalities. The foot of the patient is not easy to be perceived due to hypoesthesia, abnormal pressure distribution and tiny trauma, local tissues are in a state of low oxygen and insufficient nutrition supply for a long time due to insufficient blood supply and microcirculation disturbance, and meanwhile, the high sugar environment leads to chemotaxis of immune cells, reduced phagocytic function and reduced anti-infection capability, so that once a wound surface is formed, the wound surface is easy to repeatedly infect and is difficult to enter a normal repair process. Clinically, the diabetic foot wound surface is often characterized by more exudation, impregnation of wound edges and surrounding skin, slow or even stagnation of granulation growth, easy accompanying of peculiar smell, pain or numbness, atypical local redness, swelling and pain and repeated attack of infection symptoms, further possibility of deep tissue involvement and increased risk of osteomyelitis during the transition of the course, serious patients even need amputation treatment, and obvious influence on the life quality and medical burden of patients. Compared with the general acute wound surface, the diabetic foot has more prominent pathological characteristics that the wound surface microenvironment is in a chronic inflammation maintenance state for a long time. Infection and necrosis tissue residues can continuously activate inflammatory pathways, the proinflammatory factor level is higher, the macrophage phenotype conversion is blocked, the transition of a wound surface from an inflammatory phase to a proliferation phase is difficult, and protease (such as matrix metalloproteinase and the like) is often in excessive expression, so that extracellular matrixes and growth factors are easily degraded, the granulation tissue structure is unstable, and re-epithelialization and angiogenesis are further weakened. More particularly, bacterial biofilms are common on diabetic foot wounds. The biofilm forms a protective barrier through the extracellular polymeric matrix, so that bacteria are in a tolerant state and resist antibiotics and immune clearance, resulting in persistent and repeated recurrence of infection and poor treatment effect of conventional debridement and dressing. In addition, the diabetic foot wound surface is exudative and fragile, and the frequent dressing change process is easy to adhere to the new tissue to cause secondary injury and pain, even bleeding and inflammation rebound are caused, and the healing period is further prolonged. Aiming at diabetic foot wound surfaces, the existing dressing and local treatment materials mainly comprise gauze and cotton pad traditional covering materials, foam dressing and hydrocolloid dressing, alginate dressing, hydrogel dressing, silver-containing and iodine-containing antibacterial dressing, composite functional dressing and the like. The traditional covering material has low cost, limited moisture retention and barrier property, easy adhesion to a wound surface and frequent replacement, foam and alginate dressing have certain exudation absorption capability, but insufficient regulation and control on biological membranes and chronic inflammation microenvironment, hydrocolloid and partial hydrogel dressing can provide a wet environment, relieve pain and promote self-soluble debridement, but impregnation, insufficient fixity or curative effect fluctuation easily occur when high exudation or infection exists, antibacterial dressing can reduce surface bacterial load to a certain extent, has limited penetrating and destructive capability on a mature biological membrane, partial strong antibacterial system can introduce irritation or influence cell repair, and in addition, a plurality of dressings are easy to shift or need secondary fixation under a wet environment, and still can lead fragile granulation tissues to traction when uncovered, so that dressing change experience and stability are influenced. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide an anti-inflammatory gel for diabetic foot wound surface and a preparation method thereof. The technical effect of the invention is realized by the following technical scheme that the anti-inflammatory gel for diabetic foot wound surface comprises the following raw materials of sodium hyaluronate, carboxymethyl chitosan, tannic acid, boric acid compounds, dexamethasone, dopa