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CN-122005910-A - Piezoelectric-photothermal dual-mode composite bracket for articular cartilage repair and preparation method thereof

CN122005910ACN 122005910 ACN122005910 ACN 122005910ACN-122005910-A

Abstract

The invention belongs to the technical field of bone tissue engineering and regenerative medicine, and particularly discloses a piezoelectric-photothermal dual-mode composite bracket for articular cartilage repair and a preparation method thereof. The preparation method comprises three steps of MXene preparation, electrostatic spinning and paper folding structure construction, wherein poly-L-lactic acid and MXene nano-sheets are compounded to form a fibrous membrane, and then the fibrous membrane is subjected to hot pressing through a corrugated die to form the three-dimensional bracket with the periodic folding structure. The bracket has both piezoelectric property and photo-thermal property, promotes cartilage differentiation through piezoelectric effect, inhibits subchondral bone angiogenesis and maintains low-oxygen microenvironment through photo-thermal effect, realizes the integral repair of cartilage-subchondral bone functional units, can be used for treating cartilage defects, bone cartilage defects and osteoarthritis, and solves the problem that the cartilage homeostasis is damaged due to the promotion of bone vascularization by the traditional piezoelectric material.

Inventors

  • YAN ZUOQIN
  • WANG LIPENG
  • CAO LU
  • ZHU GUODONG
  • Zhuang Chenyang
  • ZHAO HONG
  • ZHANG QIANYI
  • QU MING

Assignees

  • 复旦大学附属中山医院

Dates

Publication Date
20260512
Application Date
20260202

Claims (10)

  1. 1. The preparation method of the piezoelectric-photothermal dual-mode composite bracket for articular cartilage repair is characterized by comprising the following steps of: S1, preparing an MXene nano-sheet, namely adding Ti 3 AlC 2 powder into hydrochloric acid solution containing LiF to stir, centrifuging, taking precipitate to wash, performing ultrasonic treatment, and performing centrifugal separation to obtain the MXene nano-sheet; S2, electrostatic spinning, namely dissolving PLLA powder in chloroform, adding the MXene nano-sheets prepared in the step 1, uniformly dispersing the MXene through magnetic stirring and ultrasonic treatment, then carrying out electrostatic spinning, and drying to obtain a fiber membrane; and S3, constructing a paper folding structure, namely placing the fiber membrane in a corrugated mold for compression and fixation to obtain a folding membrane, and performing heat welding after a plurality of layers of folding membrane are laminated to form the three-dimensional bracket.
  2. 2. The process according to claim 1, wherein in step S1, ti 3 AlC 2 powder is contained in a hydrochloric acid solution containing LiF in an amount of 0.04 to 0.08g/mL, the mixture is stirred at 40 to 45℃for 45 to 50 hours, and the precipitate is washed with deionized water.
  3. 3. The method according to claim 1, wherein in step S1, the ultrasonic treatment is carried out under the conditions of a power of 250-350W and a time of 0.5-1.5h under the protection of ice bath and inert gas.
  4. 4. The preparation method according to claim 1, wherein in the step S2, the content of PLLA in chloroform is 5-10wt%, and the mass percentage of the MXene nano-sheets relative to PLLA is 0.5-1.5%.
  5. 5. The method according to claim 1, wherein in step S2, the electrostatic spinning process parameters are voltage 12-18kV, solution flow rate 0.8-1.2 mL/h, receiving distance 14-16cm, temperature 25+ -2 ℃, humidity 30+ -5%, and spinning time 4-6h.
  6. 6. The method according to claim 1, wherein in the step S3, the compression fixing process is that the fiber film is placed between an upper corrugated die and a lower corrugated die which are preheated to 75-85 ℃, pressed for 5-15min under the pressure of 4-6 MPa by using a hydraulic press, then naturally cooled to room temperature, and the corrugated film is obtained after demoulding.
  7. 7. The method according to claim 1, wherein the conditions for the thermal fusion in step S3 are 75-85℃for 5-15S.
  8. 8. A piezoelectric-photothermal dual-mode composite stent manufactured by the manufacturing method of any one of claims 1 to 7.
  9. 9. The use of the piezoelectric-photothermal dual-mode composite scaffold of claim 8 in the preparation of a product for articular cartilage repair.
  10. 10. The use according to claim 9, wherein the articular cartilage repair comprises repair of cartilage defects, osteochondral defects, osteoarthritis.

Description

Piezoelectric-photothermal dual-mode composite bracket for articular cartilage repair and preparation method thereof Technical Field The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to a piezoelectric-photothermal dual-mode composite bracket for articular cartilage repair and a preparation method thereof. Background Cartilage defects are a significant clinical challenge in the field of regenerative medicine, affecting multiple sites such as joints, ear and nose. Defects often result in joint degeneration due to the avascular nature and limited regeneration capacity of cartilage. The existing treatment methods comprise microfracture, osteochondral transplantation and autologous chondrocyte transplantation, but have obvious limitations that microfracture is only applicable to small-area defects and fibrocartilage is produced, donor complications exist in osteochondral transplantation, and autologous chondrocyte transplantation needs multiple operations and is high in cost. The piezoelectric material can convert mechanical stimulus into electric signal to promote cartilage differentiation. However, the existing piezoelectric materials only focus on cartilage stimulation, neglecting the key role of cartilage-subchondral bone functional units. The piezoelectric effect promotes cartilage differentiation and bone vascularization, and damages the avascular hypoxia microenvironment of cartilage, resulting in local oxygen tension increase and damage to cartilage homeostasis. It is known that cartilage and subchondral bone maintain extremely low oxygen levels (subchondral bone about 7%, cartilage about 1-5%), and that excessive angiogenesis in osteoarthritis can raise local oxygen tension, compromising chondrocyte function. Therefore, it is important to develop a novel piezoelectric stent having an angiogenesis inhibiting function. Disclosure of Invention The invention provides a PLLA/MXene piezoelectric-photothermal dual-mode composite scaffold, which aims to (1) integrate a piezoelectric effect and a photothermal effect for the first time for cartilage repair, (2) promote cartilage differentiation through the piezoelectric effect, (3) inhibit subchondral bone angiogenesis through the photothermal effect, maintain a hypoxia microenvironment and (4) realize the integral repair of a cartilage-subchondral bone functional unit. In order to achieve the above purpose, the invention adopts the following specific technical scheme: In a first aspect, the invention provides a method for preparing a piezoelectric-photothermal dual-mode composite scaffold for repairing articular cartilage, which comprises the steps of preparing a fibrous membrane from poly L-lactic acid (PLLA) and MXene (containing Ti 3C2Tx) nanosheets through electrostatic spinning, and performing hot pressing through a corrugated die to form a paper folding structure three-dimensional scaffold, wherein the scaffold has piezoelectric performance and photothermal performance. The preparation method comprises the following steps: S1, preparing an MXene nano-sheet, namely adding Ti 3AlC2 powder into hydrochloric acid solution containing LiF to stir, centrifuging, taking precipitate to wash, performing ultrasonic treatment, and performing centrifugal separation to obtain the MXene nano-sheet; S2, electrostatic spinning, namely dissolving PLLA powder in chloroform, adding the MXene nano-sheets prepared in the step 1, uniformly dispersing the MXene through magnetic stirring and ultrasonic treatment, then carrying out electrostatic spinning, and drying to obtain a fiber membrane; and S3, constructing a paper folding structure, namely placing the fiber membrane in a corrugated mold for compression and fixation to obtain a folding membrane, and performing heat welding after a plurality of layers of folding membrane are laminated to form the three-dimensional bracket. Further, in the step S1, the content of Ti 3AlC2 powder in hydrochloric acid solution containing LiF is 0.04-0.08g/mL, stirring is carried out at 40-45 ℃ for 45-50h, and the precipitate is washed by deionized water. Further, in the step S1, the ultrasonic treatment is carried out under the conditions of ice bath and inert gas protection under the conditions of power of 250-350W and time of 0.5-1.5 h. Further, in the step S2, the content of the PLLA in chloroform is 5-10wt%, and the percentage of the MXene nano-sheets relative to the mass of the PLLA is 0.5-1.5%. Further, in the step S2, the electrostatic spinning process parameters are that the voltage is 12-18kV, the solution flow rate is 0.8-1.2 mL/h, the receiving distance (the distance from the needle head to the collecting plate) is 14-16cm, the temperature is 25+/-2 ℃, the humidity is 30+/-5%, and the spinning time is 4-6h. Further, in the step S3, the compression fixing process is as follows, the fiber membrane is placed between an upper corrugated die and a lower corrugated die which are preheate