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CN-122005911-A - Hemostatic sponge with antibacterial and osteogenic properties, and preparation method and application thereof

CN122005911ACN 122005911 ACN122005911 ACN 122005911ACN-122005911-A

Abstract

The invention relates to a hemostatic sponge with antibacterial and osteogenic properties, and a preparation method and application thereof. The method comprises the steps of uniformly mixing metal compound powder and bioactive ceramic powder, tabletting, calcining, grinding and sieving to obtain modified bioactive ceramic powder, uniformly mixing the modified bioactive ceramic powder with a biocompatible aqueous polymer solution, adding a cross-linking agent for cross-linking to obtain mixed gel, and freezing and freeze-drying to obtain the hemostatic sponge. The hemostatic sponge prepared by the invention has a porous structure, a shape memory function, excellent antibacterial property, high-efficiency coagulability and capability of promoting bone repair, has degradability and good biocompatibility, can synchronously solve the problems of bleeding, infection and alveolar bone absorption after tooth extraction, can reduce blood loss, shorten hemostatic time and promote alveolar bone regeneration when being used as a tooth extraction wound repair material, and is a biomedical material with great clinical value.

Inventors

  • NING CONGQIN
  • ZHANG KEXIN

Assignees

  • 上海师范大学

Dates

Publication Date
20260512
Application Date
20260302

Claims (10)

  1. 1. A method for preparing a hemostatic sponge with antibacterial and osteogenic properties, the method comprising the steps of: (1) Uniformly mixing metal compound powder and bioactive ceramic powder, tabletting, calcining, grinding and sieving to obtain modified bioactive ceramic powder; (2) Uniformly mixing the modified bioactive ceramic powder with a water-based polymer solution with biocompatibility, and then adding a cross-linking agent for cross-linking to obtain mixed gel; (3) Freezing and freeze-drying the mixed gel to obtain the hemostatic sponge with antibacterial and osteogenic properties.
  2. 2. The method of claim 1, wherein in step (1): The metal compound powder is copper oxide powder and/or zinc oxide powder, and the bioactive ceramic powder is calcium silicate phosphate powder; the mass ratio of the metal compound powder to the bioactive ceramic powder is (1-5): 95-99% The tabletting is carried out by a tablet press.
  3. 3. The method of claim 1, wherein in step (1): The calcination is calcination at 1000-1300 ℃ for 1-3 hours, and/or And sieving the mixture to obtain a sieve with 100-200 meshes.
  4. 4. The method of claim 1, wherein in step (2): The aqueous polymer solution with biocompatibility is gelatin solution; The concentration of the aqueous polymer solution with biocompatibility is 6-12 w/w%, and/or The dosage ratio of the modified bioactive ceramic powder to the biocompatible aqueous polymer solution is (0.1-2) g (2-3) mL, preferably (0.1-2) g:2.5mL, more preferably (0.25-0.75) g:2.5mL.
  5. 5. The method of claim 1, wherein in step (2): Uniformly mixing the modified bioactive ceramic powder and the aqueous polymer solution with biocompatibility in an oscillating manner for 2-20 min.
  6. 6. The method of claim 1, wherein in step (2): The cross-linking agent is glutaraldehyde solution, and the concentration of the glutaraldehyde solution is 0.1-2 v/v%; The dosage ratio of the modified bioactive ceramic powder to the glutaraldehyde solution is (0.1-2) g (2-3) mL, preferably (0.1-2) g:2.5mL, more preferably (0.25-0.75) g:2.5mL.
  7. 7. The method of claim 1, wherein in step (2): The water-based polymer solution with biocompatibility is also added with ergothioneine and magnesium glycinate.
  8. 8. The method of claim 1, wherein in step (3): The freezing temperature is-10 to-30 ℃, the freezing time is 8-16 h, and/or The temperature of freeze drying is-50 to-70 ℃, and the time of freeze drying is 20-30 hours.
  9. 9. A hemostatic sponge having antibacterial osteogenic properties produced by the production process of any one of claims 1 to 8.
  10. 10. Use of a hemostatic sponge having antibacterial osteogenic properties, obtainable by a process according to any one of claims 1 to 8, as a dental extraction wound repair material.

Description

Hemostatic sponge with antibacterial and osteogenic properties, and preparation method and application thereof Technical Field The invention belongs to the technical field of biomedical materials, and particularly relates to a hemostatic sponge with antibacterial and osteogenic properties, and a preparation method and application thereof. Background Tooth extraction is a common dental clinic operation and is used for treating diseases such as decayed tooth, periodontitis, wisdom tooth retardation and the like. However, three core complications easily occur after tooth extraction, (1) primary bleeding within 24 hours after operation and secondary bleeding after 24 hours, infection diffusion may be caused by improper treatment, (2) infection and dry slot diseases, namely premature dissolution of blood clots or exposure of alveolar bones caused by infection, severe inflammation is caused, the occurrence rate is as high as 10-30%, and (3) alveolar bone absorption, namely the horizontal bone loss rate is 29-63% and the vertical loss rate is 11-22% within 6 months after tooth extraction, seriously affecting the function and aesthetic effect of subsequent dental implant restoration. In order to solve the above problems, various biological materials have been developed in the prior art, such as nanofiber hydrogel which promotes bone regeneration by regulating macrophage polarization, but lacks instant hemostasis and long-acting antibacterial ability, injectable Tetra-PEG hydrogel which can stabilize blood clot but has low osteogenesis efficiency and cannot cope with complicated oral infection, polyphosphate crosslinked collagen scaffold which can stop bleeding efficiently but has weak antibacterial property and degradation rate which is asynchronous with bone regeneration. The existing material has the defect of single function, and can not synchronously meet the triple requirements of tooth extraction wound on instant hemostasis, long-acting antibacterial and bone regeneration promotion, so that the repair quality is poor and the complication occurrence rate is high. Therefore, development of a tooth extraction wound repair material with a multifunctional synergistic effect becomes a clinical urgent need. In summary, it is highly desirable to provide a hemostatic sponge having antibacterial osteogenic properties, and a method of making and using the same. Disclosure of Invention In order to solve one or more technical problems in the prior art, the invention provides a hemostatic sponge with antibacterial and osteogenic properties, and a preparation method and application thereof. The hemostatic sponge with antibacterial and osteogenic properties can synchronously realize hemostasis, antibacterial and osteogenic functions, has a porous structure, a shape memory function, excellent antibacterial property, high-efficiency coagulability and bone repair promoting capability, and has degradability and good biocompatibility. The hemostatic sponge can synchronously solve the problems of bleeding, infection and alveolar bone absorption after tooth extraction, can reduce blood loss, shorten hemostatic time and promote alveolar bone regeneration when being applied as a dental extraction wound repair material, and is a biomedical material with great clinical value. The present invention provides in a first aspect a method of preparing a hemostatic sponge having antibacterial osteogenic properties, the method comprising the steps of: (1) Uniformly mixing metal compound powder and bioactive ceramic powder, tabletting, calcining, grinding and sieving to obtain modified bioactive ceramic powder; (2) Uniformly mixing the modified bioactive ceramic powder with a water-based polymer solution with biocompatibility, and then adding a cross-linking agent for cross-linking to obtain mixed gel; (3) Freezing and freeze-drying the mixed gel to obtain the hemostatic sponge with antibacterial and osteogenic properties. Preferably, in the step (1), the metal compound powder is copper oxide powder and/or zinc oxide powder, the bioactive ceramic powder is calcium silicate phosphate powder, the mass ratio of the metal compound powder to the bioactive ceramic powder is (1-5): 95-99, and/or the tabletting is carried out by adopting a tablet press. Preferably, in the step (1), the calcination is performed at 1000-1300 ℃ for 1-3 hours, and/or the sieving is performed through a 100-200 mesh sieve. Preferably, in the step (2), the aqueous polymer solution with biocompatibility is gelatin solution, the concentration of the aqueous polymer solution with biocompatibility is 6-12 w/w%, and/or the dosage ratio of the modified bioactive ceramic powder to the aqueous polymer solution with biocompatibility is (0.1-2) g (2-3) mL, preferably (0.1-2) g:2.5mL, more preferably (0.25-0.75) g:2.5mL. Preferably, in the step (2), the modified bioactive ceramic powder and the aqueous polymer solution with biocompatibility are uniformly mixed in an oscillating manner,