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CN-122005915-A - Antibiotic bioactive glass and preparation method thereof

CN122005915ACN 122005915 ACN122005915 ACN 122005915ACN-122005915-A

Abstract

The invention discloses antibiotic bioactive glass and a preparation method thereof, which belong to the technical field of bioactive glass and comprise the following raw materials, by weight, 10-20 parts of bioactive glass powder, 5-15 parts of chitosan, 1-5 parts of lidocaine hydrochloride, 0.5-3 parts of vancomycin, 60-150 parts of 1% acetic acid aqueous solution, 3-8 parts of sodium tripolyphosphate solution and 0.5-2 parts of phosphosilane strontium, wherein the aims to solve the problems of weak interfacial binding force, difficult antibiotic release control and single function of the traditional material are achieved, the two-phase interfacial binding force is enhanced by introducing the phosphosilane strontium as an interfacial molecule, the structural integrity and mechanical property of the material are improved, the osteogenic differentiation is promoted, the osteoclast activity is inhibited, the material is loaded with the vancomycin and the lidocaine simultaneously, the antibacterial and analgesic functions are provided, and the material has synergistic anti-infection, analgesic and bone repair capabilities and is suitable for repairing bone defects.

Inventors

  • FANG HONGJUN
  • ZENG MEIJUN

Assignees

  • 山东秒亿通医学科技有限公司

Dates

Publication Date
20260512
Application Date
20260303

Claims (10)

  1. 1. The bioactive glass for antibiotics is characterized by comprising, by weight, 10-20 parts of bioactive glass powder, 5-15 parts of chitosan, 1-5 parts of lidocaine hydrochloride, 0.5-3 parts of vancomycin, 60-150 parts of 1% acetic acid aqueous solution, 3-8 parts of sodium tripolyphosphate solution and 0.5-2 parts of phosphosilane strontium.
  2. 2. The antibiotic bioactive glass of claim 1, wherein the preparation method of the phosphosilane strontium comprises the following steps: S1, under the protection of nitrogen, 3-bromopropyl trimethoxy silane and triethyl phosphite are reacted at a specific temperature, an intermediate I is generated through an Abuzoff reaction, and then byproducts and excessive reagents are removed through reduced pressure distillation; S2, dissolving the intermediate I obtained in the step S1 in methanol, adding hydrochloric acid solution with equal molar weight, stirring for 4 hours at room temperature to carry out hydrolytic acidification, adjusting pH to be neutral after the reaction is finished, and concentrating to obtain an active phosphosilane intermediate II; And S3, mixing the methanol solution of the intermediate II obtained in the step S2 with the methanol solution of strontium chloride, stirring and reacting for 2 hours at 40 ℃, filtering and collecting the generated white precipitate, washing with methanol and acetone, and then drying in vacuum at 40 ℃ to obtain the final product strontium phosphosilane.
  3. 3. An antibiotic bioactive glass as claimed in claim 2, wherein in said step S1, the molar ratio of (3-bromopropyl) trimethoxysilane to triethyl phosphite is 1 (1-2).
  4. 4. An antibiotic bioactive glass as claimed in claim 3, wherein in step S1, the specific reaction temperature is 120-130 ℃ and the reaction time is 6-8 hours.
  5. 5. The antibiotic bioactive glass of claim 4, wherein the concentration of the hydrochloric acid solution in step S2 is 0.5-1.5mol/L.
  6. 6. The antibiotic bioactive glass of claim 5, wherein in step S3, the molar ratio of the intermediate II to strontium chloride is 1 (0.5-1.5) in terms of phosphorus element to strontium element.
  7. 7. The antibiotic bioactive glass of claim 6, wherein in step S3, the acetone used for washing is pre-chilled to 0-4 ℃.
  8. 8. The method for preparing the bioactive glass of antibiotics according to any of claims 1 to 7, wherein the method for preparing the bioactive glass of antibiotics comprises the following steps: Dissolving strontium phosphosilane in 95% ethanol solution, stirring uniformly to form strontium phosphosilane solution, immersing bioactive glass powder in vancomycin solution to obtain antibiotic-carrying bioactive glass, immersing the antibiotic-carrying bioactive glass in the strontium phosphosilane solution for reaction to obtain surface-modified antibiotic-carrying bioactive glass, adding chitosan powder into 1% acetic acid aqueous solution, continuously stirring until chitosan is completely dissolved to obtain chitosan solution, adding lidocaine hydrochloride, continuously stirring until the solution is completely dissolved to obtain chitosan-lidocaine mixed solution, mixing the surface-modified antibiotic-carrying bioactive glass with the chitosan-lidocaine mixed solution to form uniform slurry, and then dripping the uniform slurry into 5% sodium tripolyphosphate solution for ionic crosslinking gelation to obtain the antibiotic bioactive glass.
  9. 9. The method for preparing antibiotic bioactive glass according to claim 8, wherein the mass ratio of the surface-modified antibiotic bioactive glass to the chitosan-lidocaine mixed solution is 1 (0.8-1.5).
  10. 10. The method for preparing antibiotic bioactive glass as claimed in claim 9, wherein the reaction condition of the strontium phosphosilane and the antibiotic bioactive glass powder is mild stirring reaction for 4-6 hours at 60-70 ℃.

Description

Antibiotic bioactive glass and preparation method thereof Technical Field The invention belongs to the technical field of bioactive glass, and particularly relates to antibiotic bioactive glass and a preparation method thereof. Background In recent years, bioactive glass is widely focused in the fields of bone repair and anti-infection as a biological material with great potential, and the inherent antibacterial performance of the bioactive glass can be remarkably enhanced by doping antibacterial metal elements such as silver, zinc, copper or strontium, so that the related infection risk of an implant is reduced and tissue integration is promoted, the current technical development tends to compound the bioactive glass with low-dose antibiotics or growth factors and the like, aims to synergistically promote the antibacterial effect, realize slow release of medicines and reduce the resistance of the antibiotics, and provides an important direction for developing new generation of implant materials with treatment and repair functions. The problems encountered by the current bioactive glass material are mainly that the interfacial bonding force between an inorganic phase and an organic polymer in the traditional material is weak, the structural integrity is easy to be insufficient, the release behavior after the antibiotics are loaded is difficult to control, the initial burst release or the later release is easy to occur, the long-acting antibacterial effect is influenced, meanwhile, the functions of most materials are single, the multi-functional synergy of bone activity promotion, local pain relieving and the like is difficult to realize synchronously on the basis of anti-infection, and the application potential of the materials in complex bone defect repair is limited. Disclosure of Invention Aiming at the situation, in order to overcome the defects of the prior art, the invention provides the antibiotic bioactive glass and the preparation method thereof, and the problems of insufficient integrity, short antibacterial effect duration and single function of the bioactive glass in the current market are effectively solved. The invention adopts the following technical scheme that the invention provides antibiotic bioactive glass and a preparation method thereof, wherein the antibiotic bioactive glass comprises the following raw materials, by weight, 10-20 parts of bioactive glass powder, 5-15 parts of chitosan, 1-5 parts of lidocaine hydrochloride, 0.5-3 parts of vancomycin, 60-150 parts of 1% acetic acid aqueous solution, 3-8 parts of sodium tripolyphosphate solution and 0.5-2 parts of phosphosilane strontium. Further, the preparation method of the phosphosilane strontium comprises the following steps: S1, under the protection of nitrogen, 3-bromopropyl trimethoxy silane and triethyl phosphite are reacted at a specific temperature, an intermediate I is generated through an Abuzoff reaction, and then byproducts and excessive reagents are removed through reduced pressure distillation; S2, dissolving the intermediate I obtained in the step S1 in methanol, adding hydrochloric acid solution with equal molar weight, stirring for 4 hours at room temperature to carry out hydrolytic acidification, adjusting pH to be neutral after the reaction is finished, and concentrating to obtain an active phosphosilane intermediate II; And S3, mixing the methanol solution of the intermediate II obtained in the step S2 with the methanol solution of strontium chloride, stirring and reacting for 2 hours at 40 ℃, filtering and collecting the generated white precipitate, washing with methanol and acetone, and then drying in vacuum at 40 ℃ to obtain the final product strontium phosphosilane. Further, in the step S1, the molar ratio of the (3-bromopropyl) trimethoxysilane to the triethyl phosphite is 1 (1-2). Further, in the step S1, the specific reaction temperature is 120-130 ℃ and the reaction time is 6-8 hours. Further, in the step S2, the concentration of the hydrochloric acid solution is 0.5-1.5mol/L. Further, in the step S3, the feeding molar ratio of the intermediate II to the strontium chloride is 1 (0.5-1.5) calculated by the phosphorus element and the strontium element. Further, in the step S3, the acetone used for washing is acetone precooled to 0-4 ℃. Further, the preparation method of the antibiotic bioactive glass comprises the following steps: Dissolving strontium phosphosilane in 95% ethanol solution, stirring uniformly to form strontium phosphosilane solution, immersing bioactive glass powder in vancomycin solution to obtain antibiotic-carrying bioactive glass, immersing the antibiotic-carrying bioactive glass in the strontium phosphosilane solution for reaction to obtain surface-modified antibiotic-carrying bioactive glass, adding chitosan powder into 1% acetic acid aqueous solution, continuously stirring until chitosan is completely dissolved to obtain chitosan solution, adding lidocaine hydrochloride, continuously st