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CN-122005928-A - Nerve adhesion silk fibroin hydrogel and preparation method and application thereof

CN122005928ACN 122005928 ACN122005928 ACN 122005928ACN-122005928-A

Abstract

The invention belongs to the technical fields of biomedical materials and biomedical engineering, and discloses a neural adhesion silk fibroin hydrogel, and a preparation method and application thereof. The preparation method comprises the steps of oxidizing purified peach gum to obtain aldehyde peach gum, modifying boric acid on silk fibroin to obtain borated silk fibroin, mixing the aldehyde peach gum with the borated silk fibroin solution, and promoting the synergistic effect of boric acid ester bonds and Schiff base bonds to obtain the neural adhesion silk fibroin hydrogel through arginine alkaline solution. The hydrogel has excellent biocompatibility, nerve adhesion, self-repairing and other functions, and can be applied to repairing sciatic nerve injury.

Inventors

  • XUAN HONGYUN
  • LIU ZIHAO
  • CHEN SHI
  • LU KEYU
  • YUAN HUIHUA
  • YANG YUMIN

Assignees

  • 南通大学

Dates

Publication Date
20260512
Application Date
20260121

Claims (10)

  1. 1. A method for preparing a neural adhesion silk fibroin hydrogel, which is characterized by comprising the following steps: s1, adding sodium periodate into purified peach gum under a light-shielding condition for reaction, dialyzing after the reaction is finished, and freeze-drying to obtain aldehyde peach gum; S2, dissolving silk fibroin in distilled water, adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide for activation, then adding 3-aminobenzene borate for reaction, dialyzing after the reaction is finished, and freeze-drying to obtain borated silk fibroin; s3, dissolving the aldehyde peach gum and the borated silk fibroin in distilled water, mixing the solutions, stirring uniformly, adding an arginine solution, and standing for reaction to obtain the nerve adhesion silk fibroin hydrogel.
  2. 2. The preparation method of claim 1, wherein in the step S1, the mass ratio of the purified peach gum to the sodium periodate is 1000 (690-750).
  3. 3. The preparation method according to claim 1, wherein in the step S1, the reaction time is 6 to 8 hours.
  4. 4. The preparation method according to claim 1, wherein in the step S2, the mass ratio of the silk fibroin to the 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride to the N-hydroxysuccinimide to the 3-aminobenzene borate is 500 (900-950): 500-600): 250-300.
  5. 5. The method according to claim 1, wherein in the step S2, the activation time is 15 to 30 minutes, and the reaction time is 8 to 12 hours.
  6. 6. The preparation method according to claim 1, wherein in the step S3, the dosage ratio of the aldehyde peach gum, the boronated silk fibroin and the arginine solution is 80 mg (78.4-83.2) mg (150-200) muL, and the concentration of the arginine solution is 10 wt%.
  7. 7. The method according to claim 6, wherein the amount ratio of the aldehyde peach gum to distilled water in the step S3 is 80 mg/500. Mu.L.
  8. 8. A bioadhesive silk hydrogel prepared according to the method of any one of claims 1-7.
  9. 9. A neural adhesion material, characterized in that the neural adhesion material is obtained by dehydration and drying of the neural adhesion silk fibroin hydrogel based on a boric acid ester bond and a schiff base bond according to claim 8.
  10. 10. Use of a bioadhesive silk hydrogel according to claim 8 or a bioadhesive material according to claim 9 for the preparation of a material for bioadhesive or nerve injury repair.

Description

Nerve adhesion silk fibroin hydrogel and preparation method and application thereof Technical Field The invention belongs to the technical fields of biomedical materials and biomedical engineering, and relates to a neural adhesion silk fibroin hydrogel, and a preparation method and application thereof. Background Peripheral nerve injury is a common clinical disease, usually caused by mechanical factors (such as cutting, extrusion, traction, etc.), inflammation, immune response, etc., resulting in sensory, motor and autonomic nerve dysfunction, severely affecting the quality of life of the patient. The sciatic nerve is the longest peripheral nerve in an organism and can cause motor and sensory dysfunction of the lower limb after injury. Although the traditional treatment methods such as nerve suturing, nerve transplanting and the like can repair nerves to a certain extent, the traditional treatment methods have the problems of limited donor sources, unsatisfactory postoperative function recovery and the like. Clinically, surgical treatment can be adopted for the peripheral nerve of simple mechanical injury to promote nerve repair, but for inflammatory or immune-mediated diffuse nerve injury, although further nerve injury can be reduced or blocked by immunotherapy, no specific method is available for the already-occurring nerve injury. In addition, functional recovery after nerve regeneration also faces many challenges such as muscular atrophy, dysfunction of target organs, and the like. In recent years, researchers are actively exploring and developing novel nerve repair materials and techniques against the defects of the traditional nerve repair means. The biological material is matched with the wired suture to be a new means for realizing immediate stability in the peripheral nerve repair, the suture method with the least suture line can be selected individually according to the factors of nerve diameter, bundle arrangement, defect length and the like in the clinical operation, and auxiliary adhesion, a sleeve or the biological material is adopted actively to optimize the regeneration microenvironment and improve the function recovery rate. But the problems of inflammation, scar, blood supply interference and the like caused by the suture are still not negligible. Most of suture lines in clinical application at present are non-degradable or materials with degradation rate not matched with nerve regeneration period, the non-degradable suture lines are taken out by secondary operation, secondary injury and chronic inflammation are easy to be caused, and the suture lines with over-high degradation rate are difficult to maintain long-term stability of nerve broken ends. Also, suturing does not allow for a seamless connection to the tissue, which may lead to additional trauma and complications such as scarring, inflammatory reactions, wo Leshi degeneration, and the like. In addition, the establishment of an individual suturing scheme depends on experience of operators, and the stability and repeatability of the repairing effect are further affected by lack of standardized material selection and suturing parameter guidance aiming at nerves with different diameters and different defect lengths. Disclosure of Invention The invention aims to provide a neural adhesion silk fibroin hydrogel, a preparation method and application thereof, the hydrogel prepared by the preparation method has excellent neural adhesion and self-repairing performance, and has good biocompatibility, and can be applied to the treatment of peripheral nerve defects such as sciatic nerve and the like. In a first aspect of the present invention, there is provided a method for preparing a neural adhesion silk fibroin hydrogel, comprising the steps of: s1, adding sodium periodate into purified peach gum under a light-shielding condition for reaction, dialyzing after the reaction is finished, and freeze-drying to obtain aldehyde peach gum; S2, dissolving silk fibroin in distilled water, adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide for activation, then adding 3-aminobenzene borate for reaction, dialyzing after the reaction is finished, and freeze-drying to obtain borated silk fibroin; s3, dissolving the aldehyde peach gum and the borated silk fibroin in distilled water, mixing the solutions, stirring uniformly, adding an arginine solution, and standing for reaction to obtain the nerve adhesion silk fibroin hydrogel. In some embodiments of the invention, the preparation method of the purified peach gum comprises the steps of adding sodium hydroxide into natural peach gum after soaking treatment, stirring in a water bath at 60-70 ℃ for 2-4 hours, carrying out reduced pressure suction filtration, taking filtrate, adding concentrated hydrochloric acid, dialyzing for 3-5 days, and freeze-drying to obtain the purified peach gum. Concentrated hydrochloric acid was used to neutralize residual sodium hydroxide. I